LOCUS       sequence-345216-       10215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on 
            pRK2-KmR plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, 
            Carothers JM, Zalatan JG
  TITLE     Portable bacterial CRISPR transcriptional activation enables 
            metabolic engineering in Pseudomonas putida.
  JOURNAL   Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002.
  PUBMED    33930546
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002."
COMMENT     Sequence Label: pPPC011
FEATURES             Location/Qualifiers
     source          1..10215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1118..1129
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1139..1173)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     terminator      complement(1226..1253)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1269..1340)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(1364..2950,2951..2953,2954..5440,5441..5443,
                     5444..5470))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        5766..5844
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     protein_bind    6120..6141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6156..6161,6162..6179,6180..6186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6194..6210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6199..6221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6218..6234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6218..6234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6230..6595
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV
                     LERPPPRWSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWKL"
     primer_bind     6253..6273
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6255..6273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6286..6393
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     6302..6318
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6303..6319
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6353..6369)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6353..6369)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6401..6420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6402..6420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6430..6446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6439..6461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6938..7732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6992..7011)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7602..7621
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7998..8092
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      8204..8735
                     /label=oriV
                     /note="incP origin of replication"
     CDS             join(9128..10215,1..61)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta
       61 gggccttgtg gggtcagttc cggctggggg ttcagcagcc acctgcatcg ccctaggtcc
      121 cgtaagtgcg ctgttccaga ctatcggctg tagcctaggt tgagaagaga aaagaaaacc
      181 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
      241 ttggctgaag aattcgcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
      301 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
      361 aacagttttt agcgaaaaaa ccccgccgaa gcggggtttt ttgcgtggcg gccgcttaca
      421 ggcggtggcg ataatccgcg ggagtgcgat caaactgccg cgcgaaaacg cgggagaagg
      481 tctgctgcga gacataaccc aggtccattg cgatatcaaa aatcggacgc tcggtggtgc
      541 gcaactcaac ggcggccagt aacaggcggc gttggcgaat gtaatcgcca agcgtctgat
      601 gcgtcaccgt gcggaacatt cgttgcaagt accactttga atagcctgat ttttttgcga
      661 ctacatcaat gttaagcggc tggtcaatat gctcgtcaat ccatgcgata agatcctgaa
      721 taattttctg atgggacatg ctacctccgc caccgtagat gccggagttt gctgcgattg
      781 ctgagggaat cgggtttcca tcttttagga gaccttgcat tgccttaaca ataagctcgc
      841 agtcggaatt cgtggcgaaa attggaatgg ttagttccat atttaagtac gaacgccagg
      901 cgcctttagg cacctcgact ttgatggtgt atttgcgatt ctgcgcagag ctctgacgaa
      961 cgctacaggt tactttgtaa gcctgtgaac gcgagttaga gctgatccat tcagcgatcc
     1021 cgttagcgaa gttgcttggg gcgacagtca cgtcgccagt tccgccattg tcgacgagaa
     1081 cgaactgagt aaagttagaa gcgggcccca tggtaccttt ctcctcttta atgaattcgc
     1141 tagcataata cctagggctg agctagccgt aaactgcaga ttcaccaccc tgaattgact
     1201 ctcttccggg cgctctaggt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
     1261 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     1321 tgagcctttc gttttatttg atgcctggag atccttactc gagttagtca cctcctagct
     1381 gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata agagtggcat
     1441 ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca aaatatttaa
     1501 aagcagcggg agctccaaga ttcgtcaacg taaataaatg aataatattt tctgcttgtt
     1561 cacgtattgg tttgtctcta tgtttgttat atgcactaag aactttatct aaattggcat
     1621 ctgctaaaat aacacgctta gaaaattcac tgatttgctc aataatctca tctaaataat
     1681 gcttatgctg ctccacaaac aattgttttt gttcgttatc ttctggacta cccttcaact
     1741 tttcataatg actagctaaa tataaaaaat tcacatattt gcttggcaga gccagctcat
     1801 ttcctttttg taattctccg gcactagcca gcatccgttt acgaccgttt tctaactcaa
     1861 aaagactata tttaggtagt ttaatgatta agtctttttt aacttcctta tatcctttag
     1921 cttctaaaaa gtcaatcgga tttttttcaa aggaacttct ttccataatt gtgatcccta
     1981 gtaactcttt aacggatttt aacttcttcg atttcccttt ttccacctta gcaaccacta
     2041 ggactgaata agctaccgtt ggactatcaa aaccaccata tttttttgga tcccagtctt
     2101 ttttacgagc aataagcttg tccgaatttc tttttggtaa aattgactcc ttggagaatc
     2161 cgcctgtctg tacttctgtt ttcttgacaa tattgacttg gggcatggac aatactttgc
     2221 gcactgtggc aaaatctcgc cctttatccc agacaatttc tccagtttcc ccattagttt
     2281 cgattagagg gcgtttgcga atctctccat ttgcaagtgt aatttctgtt ttgaagaagt
     2341 tcatgatatt agagtaaaag aaatattttg cggttgcttt gcctatttct tgctcagact
     2401 tagcaatcat tttacgaaca tcataaactt tataatcacc atagacaaac tccgattcaa
     2461 gttttggata tttcttaatc aaagcagttc caacgacggc atttagatac gcatcatggg
     2521 catgatggta attgttaatc tcacgtactt tatagaattg gaaatctttt cggaagtcag
     2581 aaactaattt agattttaag gtaatcactt taacctctcg aataagttta tcattttcat
     2641 cgtatttagt attcatgcga ctatccaaaa tttgtgccac atgcttagtg atttggcgag
     2701 tttcaaccaa ttggcgtttg ataaaaccag ctttatcaag ttcactcaaa cctccacgtt
     2761 cagctttcgt taaattatca aacttacgtt gagtgattaa cttggcgttt agaagttgtc
     2821 tccaatagtt tttcatcttt ttgactactt cttcacttgg aacgttatcc gatttaccac
     2881 gatttttatc agaacgcgtt aagaccttat tgtctattga atcgtcttta aggaaacttt
     2941 gtggaacaat ggcatcgaca tcataatcac ttaaacgatt aatatctaat tcttggtcca
     3001 catacatgtc tcttccattt tggagataat agagatagag cttttcattt tgcaattgag
     3061 tattttcaac aggatgctct ttaagaatct gacttcctaa ttctttgata ccttcttcga
     3121 ttcgtttcat acgctctcgc gaatttttct ggcccttttg agttgtctga ttttcacgtg
     3181 ccatttcaat aacgatattt tctggcttat gccgccccat tactttgacc aattcatcaa
     3241 caacttttac agtctgtaaa ataccttttt taatagcagg gctaccagct aaatttgcaa
     3301 tatgttcatg taaactatcg ccttgtccag acacttgtgc tttttgaatg tcttctttaa
     3361 atgtcaaact atcatcatgg atcagctgca taaaattgcg attggcaaaa ccatctgatt
     3421 tcaaaaaatc taatattgtt ttgccagatt gcttatccct aataccatta atcaattttc
     3481 gagacaaacg tccccaacca gtataacggc gacgtttaag ctgtttcatc accttatcat
     3541 caaagaggtg agcatatgtt ttaagtcttt cctcaatcat ctccctatct tcaaataagg
     3601 tcaatgttaa aacaatatcc tctaagatat cttcattttc ttcattatcc aaaaaatctt
     3661 tatctttaat aatttttagc aaatcatggt aggtacctaa tgaagcatta aatctatctt
     3721 caactcctga aatttcaaca ctatcaaaac attctatttt tttgaaataa tcttctttta
     3781 attgcttaac ggttactttt cgatttgttt tgaagagtaa atcaacaatg gctttcttct
     3841 gttcacctga aagaaatgct ggttttcgca ttccttcagt aacatatttg acctttgtca
     3901 attcgttata aaccgtaaaa tactcataaa gcaaactatg ttttggtagt actttttcat
     3961 ttggaagatt tttatcaaag tttgtcatgc gttcaataaa tgattgagct gaagcacctt
     4021 tatcgacaac ttcttcaaaa ttccatgggg taattgtttc ttcagacttc cgagtcatcc
     4081 atgcaaaacg actattgcca cgcgccaatg gaccaacata ataaggaatt cgaaaagtca
     4141 agattttttc aatcttctca cgattgtctt ttaaaaatgg ataaaagtct tcttgtcttc
     4201 tcaaaatagc atgcagctca cccaagtgaa tttgatgggg aatagagccg ttgtcaaagg
     4261 tccgttgctt gcgcagcaaa tcttcacgat ttagtttcac caataattcc tcagtaccat
     4321 ccattttttc taaaattggt ttgataaatt tataaaattc ttcttggcta gctcccccat
     4381 caatataacc tgcatatccg ttttttgatt gatcaaaaaa gatttcttta tacttttctg
     4441 gaagttgttg tcgaactaaa gcttttaaaa gagtcaagtc ttgatgatgt tcatcgtagc
     4501 gtttaatcat tgaagctgat aggggagcct tagttatttc agtatttact cttaggatat
     4561 ctgaaagtaa aatagcatct gataaattct tagctgccaa aaacaaatca gcatattgat
     4621 ctccaatttg cgccaataaa ttatctaaat catcatcgta agtatctttt gaaagctgta
     4681 atttagcatc ttctgccaaa tcaaaatttg atttaaaatt aggggtcaaa cccaatgaca
     4741 aagcaatgag attcccaaat aagccatttt tcttctcacc ggggagctga gcaatgagat
     4801 tttctaatcg tcttgattta ctcaatcgtg cagaaagaat cgctttagca tctactccac
     4861 ttgcgttaat agggttttct tcaaataatt gattgtaggt ttgtaccaac tggataaata
     4921 gtttgtccac atcactatta tcaggattta aatctccctc aatcaaaaaa tgaccacgaa
     4981 acttaatcat atgcgctaag gccaaataga ttaagcgcaa atccgcttta tcagtagaat
     5041 ctaccaattt ttttcgcaga tgatagatag ttggatattt ctcatgataa gcaacttcat
     5101 ctactatatt tccaaaaata ggatgacgtt catgcttctt gtcttcttcc accaaaaaag
     5161 actcttcaag tcgatgaaag aaactatcat ctactttcgc catctcattt gaaaaaatct
     5221 cctgtagata acaaatacga ttcttccgac gtgtatacct tctacgagct gtccgtttga
     5281 gacgagtcgc ttccgctgtc tctccactgt caaataaaag agcccctata agattttttt
     5341 tgatactgtg gcggtctgta tttcccagaa ccttgaactt tttagacgga accttatatt
     5401 catcagtgat caccgcccat ccgacgctat ttgtgccgat agctaagcct attgagtatt
     5461 tcttatccat ttttgcctcc taaaataaaa agtttaaatt aaatccataa tgagtttgat
     5521 gatttcaata atagttttaa tgacctccga aattagttta atatgcttta atttttcttt
     5581 ttcaaaatat ctcttcaaaa aatattaccc aatacttaat aataaataga ttataacaca
     5641 aaattctttt aaaaagtagt ttattttgtt atcattctat agtattaagt attgttttat
     5701 ggctgataaa tttctttgaa tttctccttg attatttgtt ataaaagtta taaaataatc
     5761 ttgttggaac cattcaaaac agcatagcaa gttaaaataa ggctagtccg ttatcaactt
     5821 gaaaaagtgg caccgagtcg gtgctttttt tgatacttct attctactct gactgcaaac
     5881 caaaaaaaca agcgctttca aaacgcttgt tttatcattt ttagggaaat taatctctta
     5941 atccttttat cattctacat ttaggcgctg ccatcttgct aaacctacta agctccacag
     6001 gatgatttcg taatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6061 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6241 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccccc
     6301 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
     6361 agttctagag cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta
     6421 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6481 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6541 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt
     6601 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6661 gccgtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     6721 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     6781 gaaagcaaat tcgacgacgt cggaattgcc agctggggcg ccctctggta aggttgggaa
     6841 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
     6901 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6961 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7021 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7081 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7141 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7201 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7261 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7321 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7381 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7441 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7501 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7561 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7621 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7681 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     7741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     7801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7861 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     7921 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     7981 tggcttccat gcgcttggac tcctgttgat agatccagta atgacctcag aactccatct
     8041 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcac
     8101 tagtgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcggg ggatcaggac
     8161 cgctgccgga gcgcaaccca ctcactacag cagagccatg tagggccgcc ggcgttgtgg
     8221 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     8281 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     8341 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     8401 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     8461 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     8521 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     8581 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     8641 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     8701 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     8761 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagccacg tagaaagcca
     8821 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     8881 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     8941 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     9001 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     9061 gcaggggatc aagatcgacg gatcgatccg gggaattaat tccggggcaa tcccgcaagg
     9121 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
     9181 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
     9241 aaccttccag tccgtcggct cgatagtcca gcaagctacg gccaagatcg agcgcgacag
     9301 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
     9361 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
     9421 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaagccaa
     9481 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
     9541 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
     9601 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
     9661 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
     9721 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
     9781 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
     9841 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
     9901 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
     9961 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
    10021 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
    10081 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
    10141 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
    10201 ctgcgaagag ttgcg
//
