LOCUS       sequence-345217-        9237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant GST-SLFN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, 
            Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, 
            Beutler B
  TITLE     SLFN2 protection of tRNAs from stress-induced cleavage is essential 
            for T cell-mediated immunity.
  JOURNAL   Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 
            10.1126/science.aba4220.
  PUBMED    33986151
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-05-14"; volume: "372(6543). pii: 372/6543/eaba4220. 
            doi"; pages: " 10.1126/science.aba4220"
COMMENT     Sequence Label: pEZT-GST-SLFN2
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..199
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     439..460
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(527..636)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     enhancer        821..1200
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1201..1404
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1355..1375
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1401..1425
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1540..1672
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1655..1674
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1717..1735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1761..2414
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2372..2394
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2421..2444
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    3612..4200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3665..3685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4083..4094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4342..4476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4379..4398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4433..4452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4505..4670
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4854..5308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4941..4960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5150..5171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5334..5438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5439..5507,5508..6299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5657..5676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6470..7058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6959..6978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7212..7229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7363..7587
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7654..8187)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8357..8362,8363..8379,8380..8385))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    8893..8941
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8966..8985
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(9026..9237,1..499,500..502))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9068..9089)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
       61 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      121 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      181 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      241 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      301 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      361 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      421 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      481 acagctcctc gcccttgctc accgggtgat caagtcttcg tcgagtgatt gtaaataaaa
      541 tgtaatttac agtatagtat tttaattaat atacaaatga tttgataata attcttattt
      601 aactataata tattgtgttg ggttgaatta aaggtccgta tactccggaa tattaatagg
      661 atcccggtcc gagcgcgcgg aattcaatat tggccattag ccatattatt cattggttat
      721 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
      781 tacatttata ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt
      841 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      901 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      961 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1021 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1081 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1141 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1201 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     1261 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1321 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     1381 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt
     1441 gcggtagttt atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg
     1501 aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
     1561 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1621 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
     1681 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
     1741 ctcgaggcgg ccgcgccacc atgagcccta tactaggtta ttggaaaatt aagggccttg
     1801 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
     1861 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
     1921 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
     1981 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
     2041 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
     2101 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
     2161 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
     2221 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
     2281 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
     2341 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
     2401 accatcctcc aaaatcggat ctggaagttc tgttccaggg gcccctggga tccccggaat
     2461 tcatgggtac tagacttgag gcaactgagc aaagcaacca cagaactcag aggaatgaca
     2521 ttcagctgga aaatgcaaag gctgctggga aaatgggcat cagtgttgat ctggaagcca
     2581 aatatgctaa gctgggtcta aatctcggag caatcacttt tggagagaag gataggaaga
     2641 aaatgaagaa ttctcacctc agaaaacagg agaatgcaaa catctctcta gctgtatgtg
     2701 ctctcctgaa ttcgggaggt ggagcaatca aggttaaaat tgaaaatgaa aattatagtc
     2761 tcactaggga tggcctggga ctagatttgg aagcctctct ttgtaaatgt ctgccctttg
     2821 tccagtggca cctggacttc acggagagcg aaggctacat ttatatctac gtgaaatcgt
     2881 ggagccaaga aatctttggg ctgcctattg gcaccctaag aaccaatttg tatgtaagga
     2941 gcatgtcatc ttctgtacaa gtgagcgccg ctgctgccct ggaatttctc caggacctgg
     3001 aggaaactgg agggagaccc tgtgtcagac cagagttgcc tgcaagcata gccttccctg
     3061 aagtggaagg agaatggcac ctggaggatt tggctgctgc attgtttaac aggacagaat
     3121 ttcagtacga ggaaactttc ccctttacca gatccagata tgttgaagtt acattgcttt
     3181 cagcgaaacg cctgcgaaaa cgcatcaaag agctcctccc tcaaactgtt tctgcttttg
     3241 caaacacgga tgggggattt ttgttcattg gtttggatgg caaaacccag caaattattg
     3301 gttttgaagc agagaagagc gatctcgtgc ttctagagag tgaaatagaa aagcacatcc
     3361 ggcagctgcc tgtcactcac ttctgtgagg agaaggagaa gatcaagtac acgtgcaaat
     3421 tcatcgaagt gcacaaatcc ggagctgtgt gtgcatatgt gtgtgcgctc agagtggaga
     3481 gattctgctg tgcagtattc gctgcagagc ccgagtcctg gcatgtggaa ggcggctgtg
     3541 tgaagaggtt taccacagag gaatgggtga aactccagat gaatgcccca tcaggttgag
     3601 gtaccaagct taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3661 actatgttgc tccttttacg ctatgtgaat acgctgcttt aatgcctttg tatcatgcta
     3721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4021 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4201 ctgcagtctg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     4261 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4321 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     4381 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4441 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctaggaga
     4501 tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
     4561 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
     4621 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt
     4681 tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc
     4741 ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat
     4801 gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc
     4861 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4921 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4981 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5041 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5101 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg
     5161 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5221 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5281 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6601 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7081 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7141 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7201 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7261 tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat
     7321 cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag
     7381 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     7441 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     7501 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     7561 gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc
     7621 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
     7681 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
     7741 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
     7801 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
     7861 atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc
     7921 gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa
     7981 gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc
     8041 acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca
     8101 tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg
     8161 cgtaacatcg ttgctgctgc gtaacatcaa acatcgaccc acggcgtaac gcgcttgctg
     8221 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc
     8281 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     8341 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt
     8401 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt
     8461 tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc
     8521 ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc ttcaggagat
     8581 cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg
     8641 catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc
     8701 tagtggttgg ctacgtaccc gtagtggcta tggcagggct tgccgccccg acgttggctg
     8761 cgagccctgg gccttcaccc gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg
     8821 cgtattggtc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa
     8881 ccccgcgttt atgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc
     8941 gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccat
     9001 ctcccggttc cgcatgctat gcatccttgt acagctcgtc catgccgaga gtgatcccgg
     9061 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     9121 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     9181 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggc
//
