LOCUS       sequence-345219-        8652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pLR5-CAG-hMCP-mScarlet-I-NLS
FEATURES             Location/Qualifiers
     source          1..8652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1054..1357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1359..1636
                     /label=chicken beta-actin promoter
     intron          1637..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2578..2600
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2662..2681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2752..2770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2822..2842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2846..2872
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2873..2884
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4022..4042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4129..4358
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4341..4360
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4430..5004,5005..5007,5008..5016)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4597..4614)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4824..4843
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5039..5842
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5102..5121)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5712..5731
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5972..6093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6009..6028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6351..6413
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6464..6484)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6464..6482)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6503..6519)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6503..6519)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6516..6538)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6527..6543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6551..6557,6558..6575,6576..6581))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6596..6617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6734..6751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6905..7493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6985..7004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7664..8455,8456..8524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8287..8306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8525..8629)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagttat taatagtaat caattacggg
     1021 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1081 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1141 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1201 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1261 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1321 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1381 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1441 attattttgt gcagcgatgg gggcgggggg gggggggggg sgcgcgccgg gggggggggg
     1501 gggggggggg ggggggggsg gggsgrggcg gagaggtgcg gcggcagcca atcagagcgg
     1561 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1621 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1681 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1741 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1801 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1861 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     1921 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1981 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     2041 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     2101 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2161 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2221 ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg
     2281 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2341 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2401 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2461 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2521 gcctcggggc tgtccgcggg gggacggctg ccttcggggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
     2761 tcactatagg gagacccaag cttggtatcg acctcggatc gtcgctagcg atatcatggg
     2821 tccaaaaaag aagagaaaag tgggataccc gtacgacgtt cccgactacg ctattgaggg
     2881 gcgccacatg ttggcagtga agatggcgtc caatttcacc caatttgtgt tggtggacaa
     2941 tgggggtaca ggggatgtga cagtagcacc ttccaacttt gccaatggta tcgcagaatg
     3001 gatttccagc aattcccgat cccaggctta caaggttaca tgttctgtca ggcagagttc
     3061 ggcgcagaat cgcaaataca ccatcaaagt cgaagtccca aaaggagctt ggcgttcata
     3121 cctgaatatg gaactcacaa tacctatttt cgcaaccaac tccgactgtg agctgatcgt
     3181 taaagcgatg cagggtttgc tgaaagatgg caaccctatc cccagtgcca ttgcagctaa
     3241 ctccgggatc tacgctatgg cgagcaactt cacacaaacc ggtgaattca ccatggtgag
     3301 taaaggagaa gctgtgatca aagagtttat gcgcttcaaa gtccatatgg aaggaagcat
     3361 gaacgggcac gagtttgaga tcgaaggtga gggtgaaggc cgcccctacg agggcacaca
     3421 gacagctaag ctgaaagtta ccaagggagg ccctctacct ttctcctggg atattctgtc
     3481 cccacagttt atgtacggga gtagagcctt cattaagcac cctgcagaca ttcctgacta
     3541 ctataagcag tcctttccag agggctttaa atgggagagg gttatgaact tcgaggatgg
     3601 cggtgcagtt accgttacac aggatacttc cttagaagat ggtactctga tctataaggt
     3661 gaagctacga ggcaccaatt ttccccccga cggacctgtg atgcagaaaa aaactatggg
     3721 ttgggaggca agcaccgagc ggctgtaccc cgaagatggt gtgctgaagg gggatattaa
     3781 aatggcccta aggcttaaag acggaggacg ctatctggca gacttcaaaa ccacctacaa
     3841 agcgaaaaaa cctgttcaaa tgccgggcgc gtataatgtg gaccgaaagt tggatatcac
     3901 atcacacaac gaagattaca ccgtggtgga acagtacgag cgcagcgagg gcagacacag
     3961 taccggaggt atggatgagc tgtacaagat ggacgagctg tacaagcctg caggcggatc
     4021 cccaaaaaag aagagaaaag tgctcgaggt taattaatga gcggccgcat agataactga
     4081 tccagtgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt gagtactccc
     4141 tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt
     4201 tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa
     4261 agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt
     4321 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact
     4381 gcaggtcgag catgcatcta gggcggccaa ttccgcccct ctcccccccc ccctctccct
     4441 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     4501 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4561 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4621 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4681 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4741 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4801 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4861 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4921 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     4981 tcctttgaaa aacacgatga taagcttgcc acaacccggg ataattcctg cagccaatat
     5041 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5101 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5161 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5221 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5281 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5341 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5401 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5461 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5521 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5581 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5641 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5701 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5761 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5821 ccttcttgac gagttcttct gaggggatca attctctaga taactgatca taatcagcca
     5881 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5941 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6061 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gagtgcattc tagttgtggt
     6121 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacata ctagttaaaa
     6181 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     6241 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     6301 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     6361 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
     6421 agtatacgcg tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa
     6481 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8641 gaaaagtgcc ac
//
