LOCUS       sequence-34522-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains TurboRFP and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtTMPV-Neo
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             227..652
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(755..777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctgatg tacgatgcat cgataccgtc gagatatcag
      121 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
      181 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
      241 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      301 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg tcgaccctag
      361 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      421 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      481 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      541 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      601 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      661 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      721 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccgga
//
