LOCUS       sequence-345222-        8151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon vector for expression of NLS-SNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pLR5-CAG-NLS-SNAP
FEATURES             Location/Qualifiers
     source          1..8151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1031..1410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1412..1689
                     /label=chicken beta-actin promoter
     intron          1690..2706
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2630..2652
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2714..2733
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2804..2822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2902..2911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2914..2934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2944..3489
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     intron          3600..3829
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3812..3831
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(3901..4475,4476..4478,4479..4487)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4068..4085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4295..4314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4510..5313
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4573..4592)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5183..5202
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5443..5564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5480..5499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5851..5913
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5964..5984)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5964..5982)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6003..6019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6003..6019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6016..6038)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6027..6043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6051..6057,6058..6075,6076..6081))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6096..6117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6234..6251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6405..6993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6485..6504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7164..7955,7956..8024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7787..7806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8025..8129)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagctgc ttcgcgatgt acgggccaga
     1021 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     1081 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1141 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1201 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1261 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1321 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1381 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1441 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1501 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc cggggggggg gggggggggs
     1561 ggggggsggg gsggggsgrg gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1621 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1681 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1741 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1801 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1861 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1921 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     1981 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2041 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2101 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2161 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2221 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2281 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2341 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2401 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2461 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2521 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2581 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2641 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2701 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagagctc
     2761 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
     2821 gggagaccca agcttggtat cgacctcgga tcgtcgctag cgatatcggc gcgccagcat
     2881 ttaaatctgt acagaccggt cgccaccatg ggcccaaaaa agaaaagaaa agttgaattc
     2941 accatggaca aagactgcga aatgaagcgc accaccctgg atagccctct gggcaagctg
     3001 gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa aggaacatct
     3061 gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc agagccactg
     3121 atgcaggcca ccgcctggct caacgcctac tttcaccagc ctgaggccat cgaggagttc
     3181 cctgtgccag ccctgcacca cccagtgttc cagcaggaga gctttacccg ccaggtgctg
     3241 tggaaactgc tgaaagtggt gaagttcgga gaggtcatca gctacagcca cctggccgcc
     3301 ctggccggca atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg
     3361 cccattctga tcccctgcca ccgggtggtg cagggcgacc tggacgtggg gggctacgag
     3421 ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg gccacagact gggcaagcct
     3481 gggctgggtc ctgcaggcgg atccgcgttt aaactcgagg ttaattaatg agcggccgca
     3541 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
     3601 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     3661 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     3721 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     3781 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     3841 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc
     3901 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     3961 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     4021 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4081 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4141 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4201 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4261 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     4321 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     4381 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     4441 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg gataattcct
     4501 gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct
     4561 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4621 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4681 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4741 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4801 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4861 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4921 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4981 caggatgatc tggacgaaga gcatcaggga ctcgcgccag ccgaactgtt cgccaggctc
     5041 aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5101 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5161 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5221 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5281 gccttctatc gccttcttga cgagttcttc tgaggggatc aattctctag ataactgatc
     5341 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5401 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5461 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5521 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt cgagtgcatt
     5581 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5641 ctagctagag cttggcgtaa tctcgacata ctagttaaaa gttttgttac tttatagaag
     5701 aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat tgtttgttga
     5761 atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa
     5821 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat gattatcttt
     5881 aacgtacgtc acaatatgat tatctttcta gggttaatct agtatacgcg tatgcggccg
     5941 cttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     6001 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6061 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6121 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6181 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8101 tagaaaaata aacaaatagg ggttccgcgc acatttcccg aaaagtgcca c
//
