LOCUS       sequence-345225-       10404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon vector for expression of 
            TetR(W43F)-3x-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pLR5-CAG-TetR_W43F-3xmNG
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1054..1357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1637..2653
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2577..2599
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2661..2680
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2751..2769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2821..2841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2845..3462
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYFHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2915..2934)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     intron          5880..6109
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6092..6111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(6182..6756,6757..6759,6760..6768)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6349..6366)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6576..6595
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6791..7594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6854..6873)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7464..7483
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7724..7845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7761..7780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   8103..8165
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8216..8236)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8216..8234)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8255..8271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8255..8271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8268..8290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8279..8295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8303..8309,8310..8327,8328..8333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8348..8369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8486..8503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8657..9245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8737..8756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9416..10207,10208..10276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10039..10058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10277..10381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagttat taatagtaat caattacggg
     1021 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1081 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1141 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1201 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1261 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1321 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1381 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1441 attattttgt gcagcgatgg gggcgggggg gggggggggg ggggcgccgg gggggggggg
     1501 gggggggggg gggggggggg gggsgrggcg gagaggtgcg gcggcagcca atcagagcgg
     1561 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1621 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1681 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1741 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1801 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1861 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     1921 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1981 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     2041 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     2101 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2161 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2221 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     2281 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2341 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2401 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2461 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2521 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     2581 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     2641 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     2701 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     2761 cactataggg agacccaagc ttggtatcga cctcggatcg tcgctagcga tatcatgggc
     2821 ccaaaaaaga aaagaaaagt tggctctaga ttagataaaa gtaaagtgat taacagcgca
     2881 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     2941 ctaggtgtag agcagcctac attgtatttc catgtaaaaa ataagcgggc tttgctcgac
     3001 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     3061 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     3121 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     3181 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     3241 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     3301 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     3361 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     3421 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ctccgctagc ctccaaaacc
     3481 gaattcacca tggtgagtaa gggagaggaa gataatatgg cctcccttcc cgctacgcac
     3541 gaactccaca tcttcgggtc aatcaacggt gttgacttcg acatggtggg ccagggcacc
     3601 ggcaatccca atgacggata cgaagaactc aatttgaaga gtacaaaggg cgatctccaa
     3661 ttctcacctt ggattctggt tccccacatt ggatacggat ttcatcagta cctgccgtac
     3721 cccgatggga tgagcccatt tcaggctgca atggtagatg gtagcggtta ccaagtacac
     3781 cgaactatgc aatttgagga cagtgcctca ctgacagtga actatcggta tacttacgaa
     3841 ggaagccaca tcaagggaga ggcacaggtc aaaggaaccg gatttccagc cgacgggcca
     3901 gtcatgacaa actccctgac cgccgcagat tggtgccgca gcaaaaagac ctatccaaat
     3961 gacaagacca ttatctcgac attcaaatgg agctacacca ccggaaacgg caaacgctat
     4021 cggtctaccg ccaggacaac ctacacattt gcaaaaccta tggccgcaaa ctatctgaaa
     4081 aaccagccga tgtatgtgtt ccgaaagacg gaattaaaac actcgaaaac agaactaaac
     4141 tttaaagagt ggcagaaagc ctttaccgac gtaatgggca tggacgagct gtataagcct
     4201 gcaggcgggt cgaggccgga ccccgcgctg gccgcactcg gaggcggcgg aagcggtggc
     4261 ggagggtcag gcggaggtgg atccatggtg agtaagggag aggaagataa tatggcctcc
     4321 cttcccgcta cgcacgaact ccacatcttc gggtcaatca acggtgttga cttcgacatg
     4381 gtgggccagg gcaccggcaa tcccaatgac ggatacgaag aactcaattt gaagagtaca
     4441 aagggcgatc tccaattctc accttggatt ctggttcccc acattggata cggatttcat
     4501 cagtacctgc cgtaccccga tgggatgagc ccatttcagg ctgcaatggt agatggtagc
     4561 ggttaccaag tacaccgaac tatgcaattt gaggacggtg cctcactgac agtgaactat
     4621 cggtatactt acgaaggaag ccacatcaag ggagaggcac aggtcaaagg aaccggattt
     4681 ccagccgacg ggccagtcat gacaaactcc ctgaccgccg cagattggtg ccgcagcaaa
     4741 aagacctatc caaatgacaa gaccattatc tcgacattca aatggagcta caccaccgga
     4801 aacggcaaac gctatcggtc taccgccagg acaacctaca catttgcaaa acctatggcc
     4861 gcaaactatc tgaaaaacca gccgatgtat gtgttccgaa agacggaatt aaaacactcg
     4921 aaaacagaac taaactttaa agagtggcag aaagccttta ccgacgtaat gggcatggac
     4981 gagctgtata agctctccag gccggacccc gcgctggccg cactcggagg cggcggaagc
     5041 ggtggcggag ggtcaggcgg aggtggatcc atggtgagta agggagagga agataatatg
     5101 gcctcccttc ccgctacgca cgaactccac atcttcgggt caatcaacgg tgttgacttc
     5161 gacatggtgg gccagggcac cggcaatccc aatgacggat acgaagaact caatttgaag
     5221 agtacaaagg gcgatctcca attctcacct tggattctgg ttccccacat tggatacgga
     5281 tttcatcagt acctgccgta ccccgatggg atgagcccat ttcaggctgc aatggtagat
     5341 ggtagcggtt accaagtaca ccgaactatg caatttgagg acggtgcctc actgacagtg
     5401 aactatcggt atacttacga aggaagccac atcaagggag aggcacaggt caaaggaacc
     5461 ggatttccag ccgacgggcc agtcatgaca aactccctga ccgccgcaga ttggtgccgc
     5521 agcaaaaaga cctatccaaa tgacaagacc attatctcga cattcaaatg gagctacacc
     5581 accggaaacg gcaaacgcta tcggtctacc gccaggacaa cctacacatt tgcaaaacct
     5641 atggccgcaa actatctgaa aaaccagccg atgtatgtgt tccgaaagac ggaattaaaa
     5701 cactcgaaaa cagaactaaa ctttaaagag tggcagaaag cctttaccga cgtaatgggc
     5761 atggacgagc tgtataaggg atccgcgttt aaactcgagg ttaattaatg agcggccgca
     5821 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
     5881 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     5941 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     6001 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     6061 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     6121 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc
     6181 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     6241 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     6301 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6361 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6421 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6481 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6541 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6601 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6661 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     6721 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg ggataattcc
     6781 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     6841 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6901 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6961 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7021 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7081 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7141 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7201 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7261 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7321 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     7381 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7441 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7501 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7561 cgccttctat cgccttcttg acgagttctt ctgaggggat caattctcta gataactgat
     7621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg tcgagtgcat
     7861 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgaca
     7921 tactagttaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     7981 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     8041 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     8101 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     8161 tagggttaat ctagtatacg cgtatgcggc cgcttaatta atccagcttt tgttcccttt
     8221 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8281 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8341 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8401 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8521 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8581 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8641 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8701 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8761 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8821 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8881 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8941 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9001 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9061 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9121 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9181 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9241 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9301 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9361 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9421 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9481 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9541 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9601 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9661 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9721 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9781 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9841 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9901 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9961 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10021 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10081 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10141 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10201 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10261 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10321 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10381 gcacatttcc ccgaaaagtg ccac
//
