LOCUS       sequence-345229-       20095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The insert is a codon optimized DNA sequence encoding VPS13D with an
            internal Halo tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20095)
  AUTHORS   Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli
            P
  TITLE     VPS13D bridges the ER to mitochondria and peroxisomes via Miro.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 
            10.1083/jcb.202010004.
  PUBMED    33891013
REFERENCE   2  (bases 1 to 20095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212021. doi"; 
            pages: " 10.1083/jcb.202010004"
COMMENT     Sequence Label: VPS13D^Halo
FEATURES             Location/Qualifiers
     source          1..20095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..938
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(198..217)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     808..827
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1114..1235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1151..1170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1205..1224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1284..1300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1284..1300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1297..1319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1308..1324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1332..1338,1339..1356,1357..1362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1377..1398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1515..1532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1686..2274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1766..1785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2445..3236,3237..3305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3068..3087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3306..3410)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3485..3504)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3676..4055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4056..4259
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4210..4230
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4256..4280
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9104..9113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9110..10000
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    18497..19085
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(18550..18570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18968..18979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(19107..19126)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    19151..19199
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      19401..19829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19488..19507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     19698..19719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(19838..19858)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(19843..20095,1..77)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(20023..20095,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     join(20085..20095,1..9)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
       61 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
      121 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
      181 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      241 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      301 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
      361 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      421 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      481 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      541 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      601 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      661 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
      721 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      781 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      841 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
      901 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa
      961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1021 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1081 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     1141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1201 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     1261 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1321 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1381 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1441 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1501 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1561 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1621 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1681 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1741 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1801 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1861 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1921 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1981 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2041 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2101 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2161 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2221 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2281 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2341 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2401 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2461 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2521 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2581 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2641 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2701 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2761 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2821 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2881 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2941 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3001 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3061 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3121 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3181 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3241 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3301 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3361 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3421 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc
     3481 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     3541 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     3601 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     3661 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     3721 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3781 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3841 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3901 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3961 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4021 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     4081 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4141 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4201 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4261 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     4321 gacaccggga ccgatccagc ctccggactc tagaatgctg gagggcctgg tcgcttgggt
     4381 cctgaacacc tacctgggga agtatgtgaa caacctgaac accgatcagc tgtccgtcgc
     4441 tctgctgaag ggcgccgtgg agctggagaa cctgccactg aagaaggatg ccctgaagga
     4501 gctggagctg cccttcgagg tgaaggccgg ctttatcggc aaggtgaccc tgcagatccc
     4561 tttttacagg ccacacgtgg acccctgggt catcagcatc agctccctgc acctgatcgg
     4621 cgcccctgag aagatccagg acttcaatga tgagaaggag aagctgctgg agagagagag
     4681 gaagaaggcc ctgctgcagg ccctggagga gaagtggaag aacgaccgcc agcagaaggg
     4741 cgagtcctac tggtattctg tgaccgccag cgtggtgaca agaatcgtgg agaatatcga
     4801 gctgaagatc caggacgtgc acctgaggtt cgaggatggc gtgaccaacc cttctcaccc
     4861 attcgccttt ggcatctgca tcaagaatgt gagcatgcag aatgccgtga acgagcccgt
     4921 gcagaagctg atgcggaaga agcagctgga tgtggccgag ttttccatct actgggacgt
     4981 ggattgtaca ctgctgggcg acctgcctca gatggagctg caggaggcaa tggcacgcag
     5041 catggagagc cggtcccacc actatgtgct ggagccagtg ttcgccagcg ccctgctgaa
     5101 gcgcaactgc tccaagaagc ctctgcgctc tcggcacagc ccacggatcg actgtgatat
     5161 ccagctggag acaatccccc tgaagctgtc ccagctgcag tacagacaga tcatggagtt
     5221 tctgaaggag ctggagcgca aggagcggca ggtgaagttc cggagatgga agcctaaggt
     5281 ggccatcagc aagaactgcc gcgagtggtg gtatttcgcc ctgaacgcca atctgtatga
     5341 gatccgggag cagagaaaga ggtgtacctg ggactttatg ctgcacaggg caagggacgc
     5401 cgtgtcttac accgataagt atttcaataa gctgaagggc ggcctgctga gcacagacga
     5461 taaggaggag atgtgccgga tcgaggagga gcagtccttt gaggagctga agatcctgcg
     5521 cgagctggtg cacgatcggt tccacaagca ggaggagctg gccgagtctc tgagagagcc
     5581 ccagtttgac agccctggcg cctgtccagg agcaccagag cctggaggag gctccggaat
     5641 gctgcagtac ctgcagtctt ggttccctgg atggggagga tggtatggac agcagacccc
     5701 agagggaaac gtggtggagg gcctgtccgc cgagcagcag gagcagtgga ttcccgagga
     5761 gatcctgggc accgaggagt tctttgaccc aacagccgat gccagctgca tgaacacata
     5821 caccaagcgc gaccacgtgt tcgccaagct gaatctgcag ctgcagcggg gcacagtgac
     5881 cctgctgcac aaggagcagg gaaccccaca gatgaacgag agcgccttca tgcagctgga
     5941 gttttccgat gtgaagctgc tggccgagtc tctgcctagg cgcaattcta gcctgctgag
     6001 cgtgcgcctg ggaggcctgt ttctgcggga cctggcaaca gagggcacca tgttccccct
     6061 gctggtgttt ccaaaccccc agaaggaagt gggcagagtg tctcagagct tcggcctgca
     6121 gaccacatcc gccgacaggt ctgatcacta cccagcagcc gaccccgatg gccccgtgtt
     6181 cgagatgctg tatgagagaa atcccgccca cagccacttc gagcggagac tgaacgtgtc
     6241 caccaggccc ctgaatatca tctacaaccc tcaggccatc aagaaggtgg ccgacttctt
     6301 ttacaagggc aaggtgcaca catctggctt cggctatcag agcgagctgg agctgagagt
     6361 ggcagaggca gcaaggcgcc agtataacaa gctgaagatg cagaccaagg ccgagatcag
     6421 acagacactg gacaggctgc tggtgggcga cttcatcgag gagtctaagc ggtggaccgt
     6481 gcggctggat atcagcgccc cacaggtcat cttccccgac gatttcaagt ttaagaatcc
     6541 tgtgctggtg gtggtggacc tgggcagaat gctgctgaca aatacccagg ataacagccg
     6601 gagaaagtcc agggacggct ccgcctctga ggagacacag ttctccgacg atgagtacaa
     6661 gacaccactg gccacccccc ctaatacacc accccctgag tcctctagct ccaacggcga
     6721 gaagacccca cccttctccg gcgtggagtt ttctgaggag cagctgcagg cccacctgat
     6781 gagcacaaag atgtacgagc ggtacagcct gtcctttatg gatctgcaga tcatggtggg
     6841 ccgggtgaag gacaactgga agcacgtgca ggacatcgat gtgggcccca cccacgtggt
     6901 ggagaagttc aatgtgcacc tgcagctgga gaggcgcctg atctacacat ccgacccaaa
     6961 gtatccagga gccgtgctgt ctggaaacct gcctgatctg aagatccaca tcaatgagga
     7021 caagatcagc gccctgaaga actgctttgc cctgctgacc acaccagaga tgaagacatc
     7081 cgatacccag atcaaggaga agatcttccc acaggaggag cagaggggct ctctgcagga
     7141 cagcgtgatg aacctgaccc agtctatcgt gctgctggag cagcacacaa gagaggtgct
     7201 ggtggagagc cagctgctgc tggccgagtt caaggtcaat tgtatgcagc tgggcgtgga
     7261 gtccaacggc agatacatct ctgtgctgaa ggtgttcggc accaacgccc actttgtgaa
     7321 gaggccatat gatgccgagg tgagcctgac agtgcacggc ctgctgctgg tggacacaat
     7381 gcagacctac ggcgccgact ttgatctgct gatggccagc cacaagaatc tgtccttcga
     7441 tatccccacc ggctccctga gagactctag ggcacagtcc cccgtgtctg gacctaatgt
     7501 ggcacacctg accgacggag ccacactgaa cgataggtct gccacctctg tgagcctgga
     7561 caagatcctg acaaaggagc aggagagcct gatcaagctg gagtatcagt tcgtgtctag
     7621 cgagtgcccc tctatgaacc tggatagcac cctgcaggtc atcagcctgc aggtgaacaa
     7681 tctggacatc atcctgaatc ctgagacaat cgtggagctg atcggcttcc tgcagaagtc
     7741 ttttccaaag gagaaggacg atctgagccc tcagccactg atgaccgatt tcgagcggag
     7801 cttccgggag cagggcacct accagtctac atatgagcag aataccgagg tggccgtgga
     7861 gatccacaga ctgaacctgc tgctgctgag gacagtgggc atggccaatc gcgagaagta
     7921 cggaaggaag atcgcaaccg caagcatcgg cggcacaaaa gtgaatgtga gcatgggctc
     7981 cacctttgac atgaacggct ccctgggctg cctgcagctg atggacctga cacaggataa
     8041 tgtgaagaac cagtatgtgg tgagcatcgg caattccgtg ggctacgaga acatcatctc
     8101 tgatatcggc tatttcgaga gcgtgtttgt gcgcatggag gacgccgccc tgaccgaggc
     8161 cctgtccttc acatttgtgg agcggtctaa gcaggagtgt ttcctgaatc tgaagatggc
     8221 cagcctgcac tacaaccact ccgccaagtt tctgaaggag ctgaccctgt ctatggacga
     8281 gctggaggag aacttcaggg gcatgctgaa gagcgccgcc acaaaggtga ccacagtgct
     8341 ggccacaaag accgccgagt actctgagat ggtgagcctg ttcgagacac ctagaaagac
     8401 aagggagccc ttcatcctgg aggagaatga gatctatggc ttcgacctgg cctcctctca
     8461 cctggatacc gtgaagctga tcctgaacat caatatcgag tcccccgtgg tgtctatccc
     8521 taggaagcca ggctcccccg agctgctggt gggacacctg ggacagatct tcatccagaa
     8581 ctttgtggcc ggcgacgatg agtcccgctc tgatcggctg caggtggaga tcaaggacat
     8641 caagctgtac agcctgaatt gcacccagct ggcaggaaga gaggcagtgg gctctgaggg
     8701 cagcaggatg ttttgtcctc caagcggctc cggctctgcc aactcccagg aggaggcaca
     8761 cttcacacgc cacgacttct ttgagtctct gcaccggggc caggcctttc acatcctgaa
     8821 caataccaca atccagttca agctggagaa gatcccaatc gagcgggaga gcgagctgac
     8881 ctttagcctg tcccccgacg atctgggcac aagctccatc atgaagatcg agggcaagtt
     8941 cgtgaatcca gtgcaggtgg tgctggccaa gcacgtgtac gagcaggtgc tgcagaccct
     9001 ggacaacctg gtgtacagcg aggatctgaa taagtatcca gcatccgcca catctagccc
     9061 atgccctgat tccccactgc caggctctgg ctcctctgga tccgccacca tggcagaaat
     9121 cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta
     9181 cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc
     9241 ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc
     9301 agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca
     9361 cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat
     9421 tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa
     9481 aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt
     9541 tgcccgcgag accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga
     9601 tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt
     9661 cgagatggac cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg
     9721 cttcccaaac gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga
     9781 atacatggac tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg
     9841 cgttctgatc ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc
     9901 tgtggacatc ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag
     9961 cgagatcgcg cgctggctgt cgacgctcga gatttccggc ctgtacaagt ccggaggatc
    10021 cggaggaccc ctgtctacat gcggcgagtc tagcgtggag agaaaggaga atggcctgtt
    10081 ttctcactcc tctctgtcca acacctctca gaagtctctg agcgtgaagg aggtgaagag
    10141 cttcacccag atccaggcca cattttgtat cagcgagctg caggtgcagc tgtccggcga
    10201 tctgaccctg ggagcacagg gcctggtgtc tctgaagttc caggattttg aggtggagtt
    10261 cagcaaggac cacccccaga cactgtctat ccagatcgcc ctgcacagcc tgctgatgga
    10321 ggacctgctg gagaagaatc ctgatagcaa gtacaagaac ctgatggtgt ccaggggagc
    10381 accaaagcct agctccctgg cacagaagga gtacctgtcc cagtcttgcc ctagcgtgtc
    10441 caacgtggaa tatcctgaca tgccacgcag cctgccatcc cacatggagg aggcccccaa
    10501 cgtgttccag ctgtaccagc gccctacctc tgccagccgg aagaagcaga aggaggtgca
    10561 ggacaaggat tatccactga ccccaccccc tagccctaca gtggatgagc caaagatcct
    10621 ggtgggcaag agcaagtttg acgattccct ggtgcacatc aacatcttcc tggtggacaa
    10681 gaagcaccca gagttttcta gctcctacaa tagagtgaac aggtccatcg acgtggattt
    10741 caattgtctg gacgtgctga tcacactgca gacctgggtg gtcatcctgg acttcttcgg
    10801 aatcggcagc accgcagaca accacgccat gagactgcca cccgagggca tcctgcacaa
    10861 tgtgaagctg gagccccacg cctctatgga gagcggcctg caggaccctg tgaacacaaa
    10921 gctggatctg aaggtgcact ccctgtctct ggtgctgaat aagaccacat ctgagctggc
    10981 caaggccaac gtgagcaagc tggtggccca cctggagatg atcgagggcg atctggccct
    11041 gcagggctcc atcggcagcc tgtccctgtc tgacctgacc tgccacggcg agttctacag
    11101 agagaggttt accacaagcg gcgaggaggc cctgatcttc cagaccttca agtatggccg
    11161 gccagatccc ctgctgagga gagagcacga catccgcgtg tctctgcgga tggccagcgt
    11221 gcagtacgtg cacacccaga gattccaggc cgaggtggtg gccttcatcc agcactttac
    11281 acagctgcag gatgtgctgg gccgccagag ggcagcaatc gagggacaga ccgtgaggga
    11341 tcaggcacag cggtgctccc gggtgctgct ggacatcgag gcaggagccc ccgtgctgct
    11401 gatccctgag tctagccgca gcaacaatct gatcgtggcc aacctgggca agctgaaggt
    11461 gaagaataag ttcctgtttg ccggcttccc cggcaccttt tctctgcagg acaaggagag
    11521 cgtgccaagc gcctccccta caggcatccc aaagcactcc ctgagaaaga ccacatctac
    11581 cgaggagcca aggggaacac actcccaggg ccagtttacc atgcctctgg ccggcatgtc
    11641 cctgggctct ctgaagagcg agttcgtgcc atctacaagc accaagcagc agggccctca
    11701 gccaacactg agcgtgggac aggagtcctc tagcccagag gatcacgtgt gcctgctgga
    11761 ctgcgtggtg gtggatctgc aggacatgga tatcttcgcc gccgagagac accctaggga
    11821 gtacagcaag gccccagagg actcctctgg cgatctgatc ttcccctctt attttgtgag
    11881 gcagaccggc ggcagcctgc tgacagagcc ttgccgcctg aagctgcagg tggagcggaa
    11941 cctggataag gagatctccc acaccgtgcc tgacatctct atccacggca atctgagctc
    12001 cgtgcactgt tccctggacc tgtacaagta taagctgatc cgcggcctgc tggagaacaa
    12061 tctgggcgag ccaatcgagg agttcatgcg gccctacgac ctgcaggacc ccagaatcca
    12121 caccgtgctg agcggcgagg tgtatacatg catgtgcttc ctgatcgata tggtgaacgt
    12181 gagcctggag ctgaaggacc ccaagaggaa ggagggagca ggctccctgg cacggttcga
    12241 cttcaagaag tgcaagctgc tgtacgagtc cttttctaat cagaccaagt ccatcaacct
    12301 ggtgagccac tccatgatgg ccttcgacac acggtatgcc ggccagaaga cctccccagg
    12361 catgacaaac gtgttctctt gcatctttca gcccgccaag aattctagca ccacacaggg
    12421 ctccatccag atcgagctgc acttccgctc taccaaggac tcctcttgtt ttacagtggt
    12481 gctgaacaat ctgcgggtgt tcctgatctt tgactggctg ctgctggtgc acgatttcct
    12541 gcacacccca agcgacatca agaagcagaa tcacgtgaca cccagcagac acaggaacag
    12601 ctcctctgag tccgccatcg tgccaaagac cgtgaagtct ggcgtggtga caaagagaag
    12661 ctccctgccc gtgtccaatg agaggcacct ggaggtgaag gtgaacgtga caggcaccga
    12721 gttcgtggtc atcgaggacg tgagctgctt tgataccaac gccatcatcc tgaagggcac
    12781 cacagtgctg acatacaagc caagattcgt ggacaggccc ttttctggca gcctgttcgg
    12841 catcgaggtg tttagctgta gactgggcaa cgagcacgat accgccctgt ccatcgtgga
    12901 ccctgtgcag atccagatgg agctggtggg caattctagc taccagaact cctctggcct
    12961 gatggacgcc ttcaattccg aggattttcc tccagtgctg gagatccagc tgcaggccct
    13021 ggatatcagg ctgtcctata acgacgtgca gctgttcctg gccatcgcca agtctatccc
    13081 tgagcaggcc aatgcagcag tgccagatag cgtggccctg gagtccgact ctgtgggcac
    13141 ctacctgcca ggagcatccc gcgtgggaga ggagatcagg gagggaacaa ggcacaccct
    13201 ggaccccgtg ctggagctgc agctggcaag actgcaggag ctgggctttt ccatggacga
    13261 ttgcaggaag gccctgctgg catgtcaggg acagctgaag aaggccgcca gctggctgtt
    13321 caagaacgca gagcccctga agtccctgtc cctggcatct accagcaggg acagccctgg
    13381 agcagtggca gcaccactga tcagcggcgt ggagatcaag gccgagtccg tgtgcatctg
    13441 tttcatcgac gattgcatgg actgtgatgt gcctctggcc gagctgacat tctccaggct
    13501 gaactttctg cagcgcgtgc ggacctctcc agagggatac gcccacttta cactgagcgg
    13561 cgactactat aatcgcgccc tgtccggctg ggagccattc atcgagccct ggccttgctc
    13621 cgtgtcttgg cagcagcagg cagcatccag actgcaccca cctaggctga agctggaggc
    13681 aaaggcaaag ccacgcctgg atatcaacat caccagcgtg ctgatcgacc agtacgtgag
    13741 cacaaaggag tcctggatgg ccgattattg caaggacgat aaggacatcg agtccgccaa
    13801 gtctgaggat tggatgggca gctccgtgga cccaccctgt ttcggccaga gcctgcccct
    13861 ggtgtacctg agaacaagga gcaccgcctc cctgaccaac ctggagcacc agatctatgc
    13921 ccgcgccgag gtgaagacac ctaagaggcg ccagcccttc gtgccttttg ccctgcggaa
    13981 tcacacaggc tgtaccctgt ggtttgccac cctgaccaca accccaacaa gagccgccct
    14041 gagccactcc ggctctcccg gagtggtgcc tgagggaaat ggcaccttcc tggacgatac
    14101 acacaacgtg tccgagtgga gggaggtgct gaccggcgag gagatcccct tcgagtttga
    14161 ggcaaggggc aagctgaggc acaggcacac acacgacctg agaatccacc agctgcaggt
    14221 gagggtgaac ggctgggagc aggtgtctcc tgtgagcgtg gataaagtgg gcaccttctt
    14281 tcggtacgcc gccccagaca agaactctag ctcctctacc atcggcagcc ccagctccag
    14341 aacaaatatc atccaccctc aggtgtattt ttctagcctg cctccagtga gggtggtgtt
    14401 cgccgtgacc atggagggct ctgccagaaa agtgatcaca gtgaggagcg ccctgatcgt
    14461 gagaaaccgg ctggagacac ccatggagct gagactggac agcccatccg cccccgataa
    14521 gcctgtggtg ctgccagcca tcatgcccgg cgattccttt gccgtgcctc tgcacctgac
    14581 atcttggaga ctgcaggcca ggcctaaggg cctgggcgtg ttcttttgca aggccccaat
    14641 ccactggacc aatgtggtga agacagccga gatctcctct agcaagaggg agtgccacag
    14701 catggacact gagaagtccc gcttctttcg gttctgcgtg gccatcaaga aggagaacta
    14761 ccctgattat atgccaagca atatcttctc tgacagcgcc aagcagatct ttcgccagcc
    14821 cggccacacc atctacctgc tgcctacagt ggtcatctgc aacctgctgc cctgtgagct
    14881 ggacttctac gtgaagggca tgcccatcaa tggcaccctg aagcctggca aggaggccgc
    14941 cctgcacaca gcagacacct ctcagaatat cgagctgggc gtgagcctgg agaacttccc
    15001 tctgtgcaag gagctgctga tcccccctgg cacccagaac tacatggtgc gcatgcggct
    15061 gtatgacgtg aatcggagac agctgaacct gacaatcagg atcgtgtgca gggcagaggg
    15121 ctctctgaag atcttcatca gcgcccctta ctggctgatc aacaagaccg gcctgccact
    15181 gatctttagg caggacaatg ccaagacaga tgcagcagga cagttcgagg agcacgagct
    15241 ggcacgctcc ctgtctcccc tgctgttttg ctatgccgat aaggagcagc ctaacctgtg
    15301 caccatgaga atcggaaggg gcatccaccc agagggaatg cctggatggt gccagggctt
    15361 ctccctggac ggaggctctg gcgtgcgggc cctgaaagtg atccagcagg gcaataggcc
    15421 tggcctgatc tacaacatcg gcatcgatgt gaagaagggc agaggcaggt acatcgacac
    15481 ctgtatggtc atctttgccc cacggtatct gctggacaac aagtcctctc acaagctggc
    15541 cttcgcccag agagagtttg caaggggaca gggaaccgca aatccagagg gctacatcag
    15601 cacactgcca ggcagctccg tggtgttcca ctggccccgc aacgactatg atcagctgct
    15661 gtgcgtgcgg ctgatggatg tgcctaattg tatctggagc ggcggcttcg aggtgaacaa
    15721 gaacaattcc tttcacatca atatgagaga caccctgggc aagtgcttct ttctgagggt
    15781 ggagatcacc ctgaggggag caacataccg gatcagcttt tccgacaccg atcagctgcc
    15841 accccctttc aggatcgaca acttttccaa ggtgcctgtg gtgttcacac agcacggagt
    15901 ggcagagcct aggctgagga ccgaggtgaa gccaatgaca agcctggact atgcctggga
    15961 tgagccaacc ctgccaccct ttatcacact gaccgtgaag ggcgccggct ctagcgagat
    16021 caactgcaat atgaacgact tccaggataa tcgccagctg tactatgaga acttcatcta
    16081 catcgccgcc acatatacct tttccggcct gcaggaggga accggccggc ccgtggcctc
    16141 taacaaggcc atcacatgtg ccgagctggt gctggacgtg agccctaaga cccagagagt
    16201 gatcctgaag aagaaggagc caggcaagag atctcagctg tggaggatga caggcaccgg
    16261 catgctggca cacgagggct cctctgtgcc acacaatccc aacaagcctt ctgccgcacg
    16321 gagcaccgag ggctccgcca tcctggatat cgcaggcctg gcagcagtga cagacaatcg
    16381 ctacgagccc ctgatgctga ggaagcctga caggcgccgg tccacaaccc agacctggtc
    16441 ttttagagag ggcaagctga catgcggcct gcacggcctg gtggtgcagg caaagggagg
    16501 cctgtccggc ctgttcgatg gagcagaggt ggtgctggga ccagacacct ctatggagct
    16561 gctgggcccc gtgcctccag agcagcagtt tatcaaccag aagatgcggc caggctctgg
    16621 catgctgagc atccgcgtga tcccagatgg accaacccgg gccctgcaga tcacagactt
    16681 ctgtcaccgc aagagctccc ggtcctatga ggtggatgag ctgcccgtga ccgagcagga
    16741 gctgcagaag ctgaagaatc ctgacacaga gcaggagctg gaggtgctgg tgagactgga
    16801 gggcggcatt ggcctgtccc tgatcaacaa ggtgcccgag gagctggtgt ttgcctctct
    16861 gaccggcatc aatgtgcact acacccagct ggccacaagc cacatgctgg agctgtccat
    16921 ccaggacgtg caggtggata accagctgat cggcacaacc cagccattca tgctgtatgt
    16981 gacccccctg agcaatgaga acgaagtgat cgagacaggc ccagcagtgc aggtgaacgc
    17041 agtgaagttt ccttctaaga gcgccctgac caatatctac aagcacctga tgatcaccgc
    17101 ccagcggttc acagtgcaga tcgaggagaa gctgctgctg aagctgctga gcttctttgg
    17161 ctacgatcag gccgagtccg aggtggagaa gtatgacgag aatctgcacg agaagaccgc
    17221 agagcaggga ggaacaccta tcagatacta ttttgagaac ctgaagatct ccatcccaca
    17281 gatcaagctg tccgtgttca cctctaataa gctgcccctg gatctgaagg ccctgaagag
    17341 cacactgggc ttccctctga tcagatttga ggacgccgtg atcaacctgg accccttcac
    17401 cagggtgcac ccctacgaga caaaggagtt catcatcaat gacatcctga agcactttca
    17461 ggaggagctg ctgtcccagg ccgccagaat cctgggctct gtggatttcc tgggcaatcc
    17521 tatgggcctg ctgaacgacg tgtccgaggg cgtgaccggc ctgatcaagt atggcaatgt
    17581 gggcggcctg atccggaacg tgacacacgg cgtgtccaat tctgccgcaa agttcgcagg
    17641 caccctgagc gatggcctgg gcaagacaat ggacaacaga caccagagcg agagagagta
    17701 catcaggtat cacgcagcca cctccggaga gcacctggtg gccggcatcc acggcctggc
    17761 acacggaatc atcggaggcc tgaccagcgt gatcacatcc accgtggagg gagtgaagac
    17821 agagggaggc gtgtctggct ttatcagcgg cctgggcaag ggcctggtgg gaacagtgac
    17881 caagcccgtg gcaggcgccc tggatttcgc aagcgagaca gcacaggccg tgcgcgacac
    17941 agccaccctg tccggaccaa ggacccaggc acagagagtg aggaagccaa ggtgctgtac
    18001 aggcccacag ggcctgctgc caaggtacag cgagtcccag gcagagggac aggagcagct
    18061 gttcaagctg accgacaaca tccaggatga gttctttatc gccgtggaga atatcgatag
    18121 ctattgcgtg ctgatctcta gcaaggccgt gtactttctg aaatccggcg actatgtgga
    18181 tcgggaggcc atcttcctgg aggtgaagta cgacgatctg tatcactgtc tggtgtccaa
    18241 ggaccacggc aaggtgtacg tgcaggtgac caagaaggcc gtgtctacat cctctggcgt
    18301 gagcatccca ggaccatccc accagaagcc catggtgcac gtgaagagcg aggtgctggc
    18361 cgtgaagctg tcccaggaga tcaactatgc caagtctctg tactatgaac agcagctgat
    18421 gctgagactg agcgaaaaca gggaacagct ggaactggat tcataaaccg gttagtaatg
    18481 agtttgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
    18541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
    18601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
    18661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
    18721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
    18781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
    18841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
    18901 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
    18961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
    19021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
    19081 cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
    19141 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
    19201 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
    19261 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
    19321 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg
    19381 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    19441 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    19501 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
    19561 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    19621 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    19681 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    19741 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga
    19801 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    19861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    19921 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    19981 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    20041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcag
//
