LOCUS       sequence-34523-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains TurboRFP and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtTMPV-Neo
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(20..146)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(174..188)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(216..337)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 agaattgcta gaattgaaaa aagtgattta atttatacca ttttaattca gctttgtaaa
       61 aatgtatcaa agagatagca aggtattcag ttttagtaaa caagataatt gctcctaaag
      121 tagccccttg aattccgagg cagtaggcat aggaattata atgcttatct atacatctgt
      181 ggcttcacta tagataagca ttataattcc tgcgctcact gtcaacagca atataccttc
      241 tcgagccttc tgttgggtta acctgaagaa gtaatcccag caagtgtttc caagatgtgc
      301 aggcaacgat tctgtaaagt actgaagcct cattcaaaca attaccctgt tatccctagt
      361 cgaggcttat ccctgcattc atctgtgccc cagtttgcta gggaggtcgc agtacttggc
      421 cacagccatc tcgtgctgct cgacgtaggt ctctttgtcg gcctccttga ttctttccag
      481 tctgtggtcc acgaagtgga agccgggcat cttgaggttc ttagcgggtt tcttggatct
      541 gtatgtggtc ttgaaggagc agtgcaggta gcccccgccc acgagcttca gggccatctg
      601 gctgtggcct ctcaggccgc cgtcagcggg gtacagcatc tcggtgttgg cctcccagcc
      661 gcgtgttttc ttctgcatca cagggccgtt ggatggg
//
