LOCUS       sequence-345235-       10203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for generating Nanog STREAMING-tag KI cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR
FEATURES             Location/Qualifiers
     source          1..10203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2032..2041
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2344..2362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2373..2391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2447..2465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2476..2494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2505..2523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2550..2568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2579..2597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2608..2626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2653..2671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2682..2700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2711..2729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2756..2774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2814..2832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2859..2877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2888..2906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2917..2935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2962..2980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2991..3009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3065..3083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3094..3112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3123..3141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3168..3186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3197..3215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3226..3244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3271..3289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3300..3318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3329..3347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3374..3392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3403..3421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3432..3450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3477..3495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3506..3524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3535..3553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3580..3598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3609..3627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3638..3656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3683..3701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3712..3730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3741..3759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3786..3804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3815..3833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3844..3862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3889..3907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3918..3936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3947..3965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3992..4010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4021..4039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4050..4068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4095..4113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4124..4142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4153..4171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4198..4216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4227..4245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4256..4274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4301..4319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4330..4348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4359..4377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4404..4422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4433..4451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4462..4480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4507..4525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4536..4554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4565..4583
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4610..4628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4639..4657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4668..4686
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4713..4731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4742..4760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4771..4789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4816..4834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4845..4863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4874..4892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4919..4937
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4948..4966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4977..4995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5022..5040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5051..5069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5080..5098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5125..5143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5154..5172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5183..5201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5228..5246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5257..5275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5286..5304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5331..5349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5360..5378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5389..5407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5434..5452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5463..5481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5492..5510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5537..5555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5566..5584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5595..5613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    5680..5705
                     /label=SA
                     /note="splice acceptor site"
     CDS             6071..6094
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6110..6133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6149..6172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8023..8043)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8023..8041)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8062..8078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8062..8078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8075..8097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8086..8102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8110..8116,8117..8134,8135..8140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8155..8176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8286..8303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8457..9045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8537..8556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9216..10007,10008..10076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9839..9858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10077..10167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggtttgaca
      661 tgcctgcaag tgcagggcat ggaccaactt actaaggtag cccgagtctt aagcaggaca
      721 caggctcttt cttcagactt gcgttaaaaa gccgcacttt tggagggaag atttccccag
      781 gcttcccaat gtgaagagca agcaagaaac gctgagtgct gaaaggaaag ccgtgtataa
      841 acagagactt tggcagcaag gtctgactct ttcatgtctg tagaaagaat ggaagaggaa
      901 actcagatcc cccacttgac ctgaaacttc ccactagaga tcgccagggt ctggaggtgc
      961 agccgtggtt aaaagatgaa taaagtgaaa tgaggtaaag cctctttttg ggggggggga
     1021 tgtctttaga tcagaggatg ccccctaagc tttccctccc tcccagtctg ggtcacctta
     1081 cagcttcttt tgcattacaa tgtccatggt ggaccctgca ggtgggatta actgtgaatt
     1141 cacagggctg gtggggcgtg ggtgccgcct gggtgcctgg gagaataggg ggtgggtagg
     1201 gtaggaggct tgagggggga ggagcaggac ctacccttta aatctatcgc cttgagccgt
     1261 tggccttcag ataggctgat ttggttggtg tcttgctctt tctgtgggaa ggctgcggct
     1321 cacttccttc tgacttcttg ataattttgc attagacatt taactcttct ttctatgatc
     1381 tttccttcta gacactgagt tttttggttg ttgcctaaaa ccttttcaga aatcccttcc
     1441 ctcgccatca cactgacatg agtgtgggtc ttcctggtcc ccacagtttg cctagttctg
     1501 aggaagcatc gaattctggg aacgcctcat caatgcctgc agtttttcat ccccaggtaa
     1561 gcgataagta ttcgatcatg cattggttag cggccgcgaa ttcgcccttg gatccggaat
     1621 gaccaccagg cattccgatg ccacgatgga ccatcaggcc atcgagctgc agaagtgacg
     1681 accacgcact tcggagtgac agaggaggat cacccctctg gccaccagag tagagcatca
     1741 gcctactcac agagcctacg gaggaccacc ccgtaggagt agagcgagga gcaccagccc
     1801 tcgcgtgtga cgcacgagca tcagccgtgc gatggtaaga tccggaatga ccaccaggca
     1861 ttcctccaag gcgatggacc atcaggccat cgaggactcg aagtgacgac cacgcacttc
     1921 gtgaaggcag aggaggatca cccctctgcc cacaggagta gagcatcagc ctactccaag
     1981 cagctacgga ggaccacccc gtagaagtag ggcgaggagc accagccctc gccatggtgg
     2041 cacgagcatc agccgtgcag ggtatagatc cggaatgacc accaggcatt cctggccaac
     2101 gatggaccat caggccatcg gcatagcgaa gtgacgacca cgcacttcag ggtagcagag
     2161 gaggatcacc cctctggcca cacgagtaga gcatcagcct actccacagg actacggagg
     2221 accaccccgt agaaagtggg cgaggagcac cagccctcgc cgtgatggca cgagcatcag
     2281 ccgtgcgtta ggaagatctc gattcgatgc agcctagtta ggggtcgact agtctactca
     2341 ctatctctat cactgatagg gaaaccttcc tctctctatc actgataggg accttttccc
     2401 ttctctatca ctgataggga cccatattct cctagtttaa cttctatctc tatcactgat
     2461 agggaaactc acttctctct atcactgata gggaccttat cccctctcta tcactgatag
     2521 ggatattata tttcctagta ttcacctcct ctctatcact gatagggatt caccattttc
     2581 tctatcactg atagggatat ttccttctct ctatcactga tagggacata tttttcccta
     2641 gtcaatcctc actctctatc actgataggg actaacaaaa ttctctatca ctgataggga
     2701 aaaccaaaca tctctatcac tgatagggat catcattttc ctagtataac catactctct
     2761 atcactgata gggatcaccc ccactctcta tcactgatag ggattacaca acttctctat
     2821 cactgatagg gactctcttt atcctagtca cattaacatc tctatcactg atagggacac
     2881 cttttcatct ctatcactga tagggacatc cattattctc tatcactgat agggacatta
     2941 actctcctag taaaatcccc atctctatca ctgataggga aacctcatca tctctatcac
     3001 tgatagggaa tctattccat ctctatcact gatagggata tcttttatcc tagtcctaac
     3061 atcatctcta tcactgatag ggaacccaaa ctttctctat cactgatagg gaccaattta
     3121 cctctctatc actgataggg aatcttctta tcctagtaca aaactaatct ctatcactga
     3181 tagggaatcc ctcccttctc tatcactgat agggatacct ttccttctct atcactgata
     3241 gggaattact tttacctagt aatacctcaa tctctatcac tgatagggaa aatcactaat
     3301 ctctatcact gatagggatt tttattattc tctatcactg atagggaact cctttaacct
     3361 agtctattcc atttctctat cactgatagg gattcactac cctctctatc actgataggg
     3421 attatttctt ctctctatca ctgataggga atttttcctt cctagtacca taaccctctc
     3481 tatcactgat agggaacacc aatattctct atcactgata gggacattat ttattctcta
     3541 tcactgatag ggaattatta ctccctagtt cttacattat ctctatcact gatagggaac
     3601 ccctctcctc tctatcactg atagggatat caatcactct ctatcactga tagggaccta
     3661 ttatacccta gtccaatata tatctctatc actgataggg accacacttc ttctctatca
     3721 ctgataggga tcaccttata tctctatcac tgatagggac actctctatc ctagtccaat
     3781 ccttttctct atcactgata gggataaaca catctctcta tcactgatag ggatacattt
     3841 tactctctat cactgatagg gattctccct ttcctagtct taacttcttc tctatcactg
     3901 atagggattt ttccaattct ctatcactga tagggaatca cttcattctc tatcactgat
     3961 agggattatt tctcccctag ttcactccta ctctctatca ctgataggga ctcccaatat
     4021 tctctatcac tgatagggat atttttctct ctctatcact gatagggaca cctcctctcc
     4081 tagttaccac aacttctcta tcactgatag ggaatactct caatctctat cactgatagg
     4141 gatttctatt tctctctatc actgataggg aataccctct acctagtaac aaatctttct
     4201 ctatcactga tagggaataa tatatctctc tatcactgat agggatctta taccctctct
     4261 atcactgata gggaccttcc acctcctagt taatcaaata tctctatcac tgatagggat
     4321 aatctacttt ctctatcact gatagggaaa ctcatccatc tctatcactg atagggattt
     4381 ttaacttcct agtatccatt ctatctctat cactgatagg gataatacaa actctctatc
     4441 actgataggg atactctcct ttctctatca ctgataggga ccttattttc cctagtataa
     4501 tccctatctc tatcactgat agggaacatt aactatctct atcactgata gggattttat
     4561 tccttctcta tcactgatag ggaacttacc tatcctagta tacccataat ctctatcact
     4621 gatagggaaa ccaaaatttc tctatcactg atagggaatc caattactct ctatcactga
     4681 tagggatttc tatctcccta gtttaaaacc aatctctatc actgataggg aacccctaaa
     4741 ctctctatca ctgataggga tttctcttat tctctatcac tgatagggac cttctctcac
     4801 ctagtctact cactatctct atcactgata gggaaacctt cctctctcta tcactgatag
     4861 ggaccttttc ccttctctat cactgatagg gacccatatt ctcctagtac actataaatc
     4921 tctatcactg atagggaaaa cataccttct ctatcactga tagggactat attttttctc
     4981 tatcactgat agggatctaa tacttcctag tactttaaat atctctatca ctgataggga
     5041 atctactaaa tctctatcac tgatagggac ccataaactt ctctatcact gatagggata
     5101 ttctttaacc tagtacaata ccattctcta tcactgatag ggacccccct aattctctat
     5161 cactgatagg gaattttttt cctctctatc actgataggg attatctctt ccctagttcc
     5221 tcttaaatct ctatcactga tagggatctc actacttctc tatcactgat agggaaactc
     5281 ataactctct atcactgata gggaaaatta tcttcctagt aaaacaccta tctctatcac
     5341 tgatagggat attataccat ctctatcact gatagggatt tatcattttc tctatcactg
     5401 atagggattt ttaccatcct agttaaaaat atctctctat cactgatagg gatcactaac
     5461 tctctctatc actgataggg aactattcta ttctctatca ctgataggga cactttttaa
     5521 cctagtacat tacccatctc tatcactgat agggaaccta tctcttctct atcactgata
     5581 gggatatttt ttcatctcta tcactgatag ggaaaacttt acacctagga ttaccctgtt
     5641 atccctactc gacatcgatt agctgatacg ataaagcttc tgacctcttc tcttcctccc
     5701 acagggatgt ctgagctacg atacagagat cctcacagtt gagtacggat ttctgcccat
     5761 tggcaaaata gtcgaggaaa ggattgaatg cacagtttac acagtggaca agaatggctt
     5821 tgtctataca cagccaatag cccagtggca taaccgagga gagcaagagg tattcgagta
     5881 ttgtctcgaa gatgggagta tcatccgagc caccaaagac cacaaattca tgacaacaga
     5941 cggacaaatg ctgcctattg acgaaatatt tgagagaggt ttggatctga agcaggttga
     6001 cgggctgccc gccggagccg gtgccggagc tgcaagcggg aaagagaaaa cacatgtttc
     6061 ccggcctagg gattacaagg atgacgacga taagggcact gcaagcagtg actataagga
     6121 tgatgatgac aaagggtctg gtgggggcga ctataaggat gacgatgaca agcggcctag
     6181 gcttaatagc aacatggacg cagaccttta tggatataag tgggctcgcg acaatgtagg
     6241 acagagcggc gctactattt atcggctcta tggaaaaccc gatgctcccg agctgttcct
     6301 gaagcacggc aagggatccg tagcaaacga cgttacagac gaaatggtac gactcaactg
     6361 gttgacagaa tttatgcccc tgcccactat caagcacttc ataagaactc ctgatgacgc
     6421 ttggctgctg acaaccgcaa tccccggtaa aacagcattc caggtccttg aagagtatcc
     6481 agactctggt gagaatattg tggacgcact ggcagtattc ctcaggcggc tccacagtat
     6541 tcctgtttgc aattgtccct ttaactctga cagggtattc aggctcgccc aggcccaatc
     6601 acgaatgaat aatggcttgg ttgatgcaag cgatttcgat gatgaacgca atggctggcc
     6661 tgttgaacaa gtttggaaag aaatgcacaa gctcctgccc ttttcacccg attctgtagt
     6721 gactcacggg gacttcagcc tcgacaattt gatcttcgat gaaggaaagc ttatcggctg
     6781 catagatgtt gggcgcgttg ggatagccga cagataccag gacctggcca tattgtggaa
     6841 ttgtctcggc gagttttccc ctagcttgca aaaacggctc tttcagaagt atggcataga
     6901 taaccccgac atgaataaac ttcaattcca tctgatgctt gacgagttct tcggaactgc
     6961 tggagctgga gctggggctg taaagggagg aggtggatct ggtggaggag gtagtggagg
     7021 aggtggaagt ggtgggggag taaagatcat cagtcgaaaa tccctgggca cccaaaatgt
     7081 atacgatatt ggggtcgaga aagatcacaa ttttctcctg aagaatggtc tggttgcaag
     7141 caattgcttc gagaactatt cttgcttaca agggtctgct actgagatgc tctgcacaga
     7201 ggctggtaag gaattcagtc cccgaagaac ccagtaaatt agggtaagag tagaaaaaac
     7261 gggctgaagg gttattgctt ctttatgaac cgtagtagtc attaacataa gcgggtgtcc
     7321 ttatcactct tctggaaacc cctctgagtt tgaccggtga tacgttggcc ttctagtctg
     7381 aaatagagat ccgggacaca ggacggagca atataaggct cttgtgtgta tttgagaacc
     7441 taaatatttg ggctctttgg aatatgttcg ggggcagtga gtggtgtctt cagtagcagg
     7501 gagacctaac atcctagctt ggaatgagca ccccattttt ttggtgaggt tatacagtta
     7561 gtttgcaatt tgcccaaatg gccgttttct ccagcagaag cagttagcat ttctctcaac
     7621 ctttgggaat atggatgtca cttagcagtt ctgggtttgt gagctcagtg ctccttccaa
     7681 accccaaaca agtgtacaca gtttaagtca ttcattctta tagctagcat ctggagccaa
     7741 ggtctgaagc tcattgtctg tacagagtca gcccagtact caggcttgta aatggtagaa
     7801 ccaagaggct gctaggctgt gtgtgccacg gtgctcaggt gcgtgttgtg atgctcgggt
     7861 gtgtgcagcg atgctcgggt gtgtgcagtg atgctcgggt gtgtgcagcg gtgcttgggt
     7921 gtgtggagcg ctaggaagtg tgtgtcccct tttttctgag cttttaaatt aatgtctgtc
     7981 atcttgcctg cacttgcagg catgtcaaac cagcttttgt tccctttagt gagggttaat
     8041 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcc
     8281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcggctg
     9361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10081 ttccttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10141 ttgaatgtat ttagaaaaat aaacaaatag ggattccgcg cacatttccc cgaaaagtgc
    10201 cac
//
