LOCUS       sequence-34524-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPVIR
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aatatggcca caaccatgag tagactggac aagagcaaag tcataaacgg
      181 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
      241 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
      301 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
      361 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
      421 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
      481 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
      541 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
      601 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
      661 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
      721 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
      781 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
      841 gctgcctgct gacgctcttg acgattt
//
