LOCUS       sequence-345247-       10491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for generating Usp5 STREAMING-tag KI cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pTV-Usp5-5prime-1000-24MS96T-3F_NeoR
FEATURES             Location/Qualifiers
     source          1..10491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1330..1341)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      2155..2164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2467..2485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2496..2514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2525..2543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2570..2588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2599..2617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2628..2646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2673..2691
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2702..2720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2731..2749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2776..2794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2805..2823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2879..2897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2908..2926
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2937..2955
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2982..3000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3011..3029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3040..3058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3085..3103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3114..3132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3143..3161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3188..3206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3217..3235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3246..3264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3291..3309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3320..3338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3349..3367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3394..3412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3423..3441
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3452..3470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3497..3515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3526..3544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3555..3573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3600..3618
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3629..3647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3658..3676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3703..3721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3732..3750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3761..3779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3835..3853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3864..3882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3909..3927
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3938..3956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3967..3985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4012..4030
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4041..4059
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4070..4088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4115..4133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4173..4191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4218..4236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4247..4265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4276..4294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4321..4339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4350..4368
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4379..4397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4424..4442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4453..4471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4482..4500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4527..4545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4556..4574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4585..4603
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4630..4648
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4659..4677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4688..4706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4733..4751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4762..4780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4791..4809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4836..4854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4865..4883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4894..4912
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4939..4957
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4968..4986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4997..5015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5042..5060
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5071..5089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5100..5118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5145..5163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5174..5192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5203..5221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5248..5266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5277..5295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5306..5324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5351..5369
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5380..5398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5409..5427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5454..5472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5483..5501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5512..5530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5557..5575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5586..5604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5615..5633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5660..5678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5689..5707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5718..5736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    5803..5828
                     /label=SA
                     /note="splice acceptor site"
     CDS             6194..6217
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6233..6256
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6272..6295
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8311..8331)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8311..8329)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8350..8366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8350..8366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8363..8385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8374..8390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8398..8404,8405..8422,8423..8428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8443..8464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8574..8591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8745..9333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8825..8844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9504..10295,10296..10364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10127..10146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10365..10455)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggtttgaca
      661 tgcctgcaag tgcaggacag agcagtcagg tctgcgggag ccttcgtatt taaccactaa
      721 ttcattcaca ttacttaccg ggccccagct cgacctctgc ccgaacacca gctagagaaa
      781 taactcccaa agcccggagt ttcaaacttc cctccacgcc ccctgccccg accctattgg
      841 ctaagccgaa gcagactctc ataggccagt tgtggaaaat gcgccttccg gagcagaaag
      901 ccattggtct gaaggatgtc tatcatgagg actggccaat gggctgagga ctggcttgcc
      961 ctccggtggt ggctccgtga gctggggact tttgtttagt atcagcctga cagcggtgga
     1021 cttgatgggt gccactcggc ctttctgttt cattaaacaa aatagaatga gttatcagac
     1081 tgtatgcaca aagccagctg gagacggagt taatatggtt tgtctaaagc gaaacagcca
     1141 tccttattgg ttgacggagt tatcacgcat ctctcaaaat cgcctcattg actactgctt
     1201 ttcaaatacc aagacaagtg ctttctgatt ggacgagtcc ttggcaaagt ttcagtaatc
     1261 aatgcgttta aacaattcac agtttctgct ctgcacgtaa ggcattctag agtccttgaa
     1321 tcacagctcc tgcaactccc agccctcctg gaaccgccac atgagcactt gccctgggag
     1381 agcaggattg gtcctttcgg ccatcagttg tacccgcggc gctttttatt ggtattcgct
     1441 gagtgggggc agagggtgga gcgccaggag gcaggctggg tccaactgcc gatctcgcgc
     1501 gtcttgcctt tagcgcatgc gcacactgag tcgcgctcat tggatgcgag agacggaggg
     1561 gggcggtact aggtacagtg ggagctgctg tgtgaggagg agctgctgcc ggtgtcatgg
     1621 cggagctgag tgaagaggcg ctgctgtcag tgttaccgac gatccgtgtc cccaagcagg
     1681 taagcgataa gtattcgatc atgcattggt tagcggccgc gaattcgccc ttggatccgg
     1741 aatgaccacc aggcattccg atgccacgat ggaccatcag gccatcgagc tgcagaagtg
     1801 acgaccacgc acttcggagt gacagaggag gatcacccct ctggccacca gagtagagca
     1861 tcagcctact cacagagcct acggaggacc accccgtagg agtagagcga ggagcaccag
     1921 ccctcgcgtg tgacgcacga gcatcagccg tgcgatggta agatccggaa tgaccaccag
     1981 gcattcctcc aaggcgatgg accatcaggc catcgaggac tcgaagtgac gaccacgcac
     2041 ttcgtgaagg cagaggagga tcacccctct gcccacagga gtagagcatc agcctactcc
     2101 aagcagctac ggaggaccac cccgtagaag tagggcgagg agcaccagcc ctcgccatgg
     2161 tggcacgagc atcagccgtg cagggtatag atccggaatg accaccaggc attcctggcc
     2221 aacgatggac catcaggcca tcggcatagc gaagtgacga ccacgcactt cagggtagca
     2281 gaggaggatc acccctctgg ccacacgagt agagcatcag cctactccac aggactacgg
     2341 aggaccaccc cgtagaaagt gggcgaggag caccagccct cgccgtgatg gcacgagcat
     2401 cagccgtgcg ttaggaagat ctcgattcga tgcagcctag ttaggggtcg actagtctac
     2461 tcactatctc tatcactgat agggaaacct tcctctctct atcactgata gggacctttt
     2521 cccttctcta tcactgatag ggacccatat tctcctagtt taacttctat ctctatcact
     2581 gatagggaaa ctcacttctc tctatcactg atagggacct tatcccctct ctatcactga
     2641 tagggatatt atatttccta gtattcacct cctctctatc actgataggg attcaccatt
     2701 ttctctatca ctgataggga tatttccttc tctctatcac tgatagggac atatttttcc
     2761 ctagtcaatc ctcactctct atcactgata gggactaaca aaattctcta tcactgatag
     2821 ggaaaaccaa acatctctat cactgatagg gatcatcatt ttcctagtat aaccatactc
     2881 tctatcactg atagggatca cccccactct ctatcactga tagggattac acaacttctc
     2941 tatcactgat agggactctc tttatcctag tcacattaac atctctatca ctgataggga
     3001 caccttttca tctctatcac tgatagggac atccattatt ctctatcact gatagggaca
     3061 ttaactctcc tagtaaaatc cccatctcta tcactgatag ggaaacctca tcatctctat
     3121 cactgatagg gaatctattc catctctatc actgataggg atatctttta tcctagtcct
     3181 aacatcatct ctatcactga tagggaaccc aaactttctc tatcactgat agggaccaat
     3241 ttacctctct atcactgata gggaatcttc ttatcctagt acaaaactaa tctctatcac
     3301 tgatagggaa tccctccctt ctctatcact gatagggata cctttccttc tctatcactg
     3361 atagggaatt acttttacct agtaatacct caatctctat cactgatagg gaaaatcact
     3421 aatctctatc actgataggg atttttatta ttctctatca ctgataggga actcctttaa
     3481 cctagtctat tccatttctc tatcactgat agggattcac taccctctct atcactgata
     3541 gggattattt cttctctcta tcactgatag ggaatttttc cttcctagta ccataaccct
     3601 ctctatcact gatagggaac accaatattc tctatcactg atagggacat tatttattct
     3661 ctatcactga tagggaatta ttactcccta gttcttacat tatctctatc actgataggg
     3721 aacccctctc ctctctatca ctgataggga tatcaatcac tctctatcac tgatagggac
     3781 ctattatacc ctagtccaat atatatctct atcactgata gggaccacac ttcttctcta
     3841 tcactgatag ggatcacctt atatctctat cactgatagg gacactctct atcctagtcc
     3901 aatccttttc tctatcactg atagggataa acacatctct ctatcactga tagggataca
     3961 ttttactctc tatcactgat agggattctc cctttcctag tcttaacttc ttctctatca
     4021 ctgataggga tttttccaat tctctatcac tgatagggaa tcacttcatt ctctatcact
     4081 gatagggatt atttctcccc tagttcactc ctactctcta tcactgatag ggactcccaa
     4141 tattctctat cactgatagg gatatttttc tctctctatc actgataggg acacctcctc
     4201 tcctagttac cacaacttct ctatcactga tagggaatac tctcaatctc tatcactgat
     4261 agggatttct atttctctct atcactgata gggaataccc tctacctagt aacaaatctt
     4321 tctctatcac tgatagggaa taatatatct ctctatcact gatagggatc ttataccctc
     4381 tctatcactg atagggacct tccacctcct agttaatcaa atatctctat cactgatagg
     4441 gataatctac tttctctatc actgataggg aaactcatcc atctctatca ctgataggga
     4501 tttttaactt cctagtatcc attctatctc tatcactgat agggataata caaactctct
     4561 atcactgata gggatactct cctttctcta tcactgatag ggaccttatt ttccctagta
     4621 taatccctat ctctatcact gatagggaac attaactatc tctatcactg atagggattt
     4681 tattccttct ctatcactga tagggaactt acctatccta gtatacccat aatctctatc
     4741 actgataggg aaaccaaaat ttctctatca ctgataggga atccaattac tctctatcac
     4801 tgatagggat ttctatctcc ctagtttaaa accaatctct atcactgata gggaacccct
     4861 aaactctcta tcactgatag ggatttctct tattctctat cactgatagg gaccttctct
     4921 cacctagtct actcactatc tctatcactg atagggaaac cttcctctct ctatcactga
     4981 tagggacctt ttcccttctc tatcactgat agggacccat attctcctag tacactataa
     5041 atctctatca ctgataggga aaacatacct tctctatcac tgatagggac tatatttttt
     5101 ctctatcact gatagggatc taatacttcc tagtacttta aatatctcta tcactgatag
     5161 ggaatctact aaatctctat cactgatagg gacccataaa cttctctatc actgataggg
     5221 atattcttta acctagtaca ataccattct ctatcactga tagggacccc cctaattctc
     5281 tatcactgat agggaatttt tttcctctct atcactgata gggattatct cttccctagt
     5341 tcctcttaaa tctctatcac tgatagggat ctcactactt ctctatcact gatagggaaa
     5401 ctcataactc tctatcactg atagggaaaa ttatcttcct agtaaaacac ctatctctat
     5461 cactgatagg gatattatac catctctatc actgataggg atttatcatt ttctctatca
     5521 ctgataggga tttttaccat cctagttaaa aatatctctc tatcactgat agggatcact
     5581 aactctctct atcactgata gggaactatt ctattctcta tcactgatag ggacactttt
     5641 taacctagta cattacccat ctctatcact gatagggaac ctatctcttc tctatcactg
     5701 atagggatat tttttcatct ctatcactga tagggaaaac tttacaccta ggattaccct
     5761 gttatcccta ctcgacatcg attagctgat acgataaagc ttctgacctc ttctcttcct
     5821 cccacaggga tgtctgagct acgatacaga gatcctcaca gttgagtacg gatttctgcc
     5881 cattggcaaa atagtcgagg aaaggattga atgcacagtt tacacagtgg acaagaatgg
     5941 ctttgtctat acacagccaa tagcccagtg gcataaccga ggagagcaag aggtattcga
     6001 gtattgtctc gaagatggga gtatcatccg agccaccaaa gaccacaaat tcatgacaac
     6061 agacggacaa atgctgccta ttgacgaaat atttgagaga ggtttggatc tgaagcaggt
     6121 tgacgggctg cccgccggag ccggtgccgg agctgcaagc gggaaagaga aaacacatgt
     6181 ttcccggcct agggattaca aggatgacga cgataagggc actgcaagca gtgactataa
     6241 ggatgatgat gacaaagggt ctggtggggg cgactataag gatgacgatg acaagcggcc
     6301 taggcttaat agcaacatgg acgcagacct ttatggatat aagtgggctc gcgacaatgt
     6361 aggacagagc ggcgctacta tttatcggct ctatggaaaa cccgatgctc ccgagctgtt
     6421 cctgaagcac ggcaagggat ccgtagcaaa cgacgttaca gacgaaatgg tacgactcaa
     6481 ctggttgaca gaatttatgc ccctgcccac tatcaagcac ttcataagaa ctcctgatga
     6541 cgcttggctg ctgacaaccg caatccccgg taaaacagca ttccaggtcc ttgaagagta
     6601 tccagactct ggtgagaata ttgtggacgc actggcagta ttcctcaggc ggctccacag
     6661 tattcctgtt tgcaattgtc cctttaactc tgacagggta ttcaggctcg cccaggccca
     6721 atcacgaatg aataatggct tggttgatgc aagcgatttc gatgatgaac gcaatggctg
     6781 gcctgttgaa caagtttgga aagaaatgca caagctcctg cccttttcac ccgattctgt
     6841 agtgactcac ggggacttca gcctcgacaa tttgatcttc gatgaaggaa agcttatcgg
     6901 ctgcatagat gttgggcgcg ttgggatagc cgacagatac caggacctgg ccatattgtg
     6961 gaattgtctc ggcgagtttt cccctagctt gcaaaaacgg ctctttcaga agtatggcat
     7021 agataacccc gacatgaata aacttcaatt ccatctgatg cttgacgagt tcttcggaac
     7081 tgctggagct ggagctgggg ctgtaaaggg aggaggtgga tctggtggag gaggtagtgg
     7141 aggaggtgga agtggtgggg gagtaaagat catcagtcga aaatccctgg gcacccaaaa
     7201 tgtatacgat attggggtcg agaaagatca caattttctc ctgaagaatg gtctggttgc
     7261 aagcaattgc ttcgcgggag accgggtcca taaagacgag tgcgctttct ctttcgacac
     7321 gccggtaagc ccattcccca cgcccgcgac gaccacgact tccttccatt gccctggtca
     7381 ttcggccagg cctgcaaacc ttgggctacc gcctccttgc gatgcaccct gggacttgta
     7441 gttttctcca tacctctctg cttttgcttt tcattctctg tagctgtagt atgactacca
     7501 ctcccggaag ctacagctct cgccttgccc actcctggtc tcgtgaagca ttatgggcat
     7561 tgtagttggc catgtcccta gttcctattc taagttcgaa ttgtaacccg tacggcgaac
     7621 tacatccccc aggcgactcc cagaaaaacc cttgaatgtg gccttgcacg ttgcgcaggg
     7681 ctctccgggt tttgtagttt catgtgggag gagtaggggc gactccccgc tgttcagggc
     7741 aagggttgag attgggaagg tatgtatcct gcagcttcta gtctggaaga ggatgccaat
     7801 ctgtgaagaa gtccgattta gagaaacact gagttaaata gcctccacta gtggttaatt
     7861 tggacagggg atgaggaggt gggccattgg tcagggagga ggaaaaatac ccaatggaga
     7921 gttctagtcg ttttttcctt tgaagtgaac tatagccgcg acccctccca ttcctccgtc
     7981 cttcacaggt tgccgagaca acccctctct caagtattcc ttagaaaggg tttctttgga
     8041 ggatgctaag gcccctttct cgtcttgctc ccactcttgt ctagttagac aaagccgcca
     8101 cccacaccgt gcttcacgtt ctgcaggctc caccttgtac ctcccttcct gcagttctgc
     8161 cttcctgtgt catcttcagc cagagaggag ccttcaggcc ctaactgaaa gggccgcagt
     8221 ttggttggga tccgccccct tcatcacccc tcccctctag cggttgttgg gccccctgca
     8281 cttgcaggca tgtcaaacca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     8341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcggctgcc acgctcaccg
     9661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt ccttttcaat
    10381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10441 agaaaaataa acaaataggg attccgcgca catttccccg aaaagtgcca c
//
