LOCUS       sequence-345258-        9463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild type codon-optimized HPDL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi 
            G, Jones DR, Kimmelman AC, Pacold ME
  TITLE     The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 
            synthesis pathway.
  JOURNAL   Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w.
  PUBMED    34471290
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w."
COMMENT     Sequence Label: pLVU-coHPDL WT
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..164
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(246..266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        251..580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      431..566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        628..675
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             694..1092
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1179..1412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1484..1605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1521..1540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1575..1594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1645..1780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1800..1819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1801..1819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1829..1846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1829..1845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1838..1860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1987..2442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2074..2093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2284..2305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2468..2572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2573..2641,2642..3433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2791..2810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3604..4192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4093..4112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4346..4363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4480..4501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4516..4521,4522..4539,4540..4546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4554..4570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4559..4581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4578..4594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4578..4594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4613..4633
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4615..4633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4661..4887
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4888..5068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5115..5240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5733..5966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6151..6195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6461..7672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7509..7528
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    7732..7756
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7760..7769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8876..8900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8953..8955,8956..8958,8959..9463,1..209)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8998..9019)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9259..9278)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
       61 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      121 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      181 ctctcggcat ggacgagctg tacaagtaac gtaccggtta gtaatgagtt tggaattaat
      241 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
      301 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      481 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
      541 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
      601 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
      661 atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa
      721 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
      781 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
      841 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
      901 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
      961 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     1021 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     1081 tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
     1141 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     1201 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     1261 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     1321 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     1381 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     1441 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     1501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     1621 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     1681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     1741 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     1801 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1861 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1921 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1981 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2041 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2101 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2161 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2221 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2281 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2341 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2401 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     2461 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4381 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4441 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4501 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     4561 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     4621 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     4681 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     4741 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     4801 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     4861 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     4921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5041 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5101 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5161 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5221 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5281 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5341 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5401 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5461 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5521 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     5581 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5641 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5701 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     5761 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     5821 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     5881 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     5941 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6001 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6061 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6121 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6181 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6241 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6301 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6361 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6421 tccattcgat tagtgaacgg atctcgacgg tatcggatct ggcctccgcg ccgggttttg
     6481 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     6541 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     6601 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     6661 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     6721 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     6781 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     6841 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     6901 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     6961 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     7021 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     7081 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     7141 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     7201 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     7261 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     7321 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     7381 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     7441 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     7501 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     7561 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     7621 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     7681 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
     7741 tacaaaaaag caggcttcac catggcggcg ccagcgctcc gtctgtgtca cattgcgttc
     7801 cacgttccgg ccggtcaacc actggcgcgt aatctgcagc gtctgtttgg ctttcagcca
     7861 ctggccagcc gtgaagttga tggttggcgc cagctggcgc tccgtagtgg cgatgcggtt
     7921 tttctggtga atgagggtgc cggcagtggc gaaccactgt atggtcttga tccacgccat
     7981 gcggtgccaa gtgcgaccaa tctgtgcttc gatgtggcgg atgccggtgc ggccacccgc
     8041 gaactggcgg cgctgggttg tagtgttccg gttccgccgg ttcgtgttcg cgatgcgcaa
     8101 ggtgccgcga cgtatgcggt tgttagcagc ccggcgggta ttctgagtct cacgctgctg
     8161 gaacgtgcgg gttatcgtgg tccatttctg ccgggctttc gtccggttag cagtgccccg
     8221 ggtccgggtt gggttagtcg tgttgaccat ctgacgctgg cgtgcacccc gggtagtagc
     8281 ccaacgctgc tccgttggtt tcacgactgt ctgggcttct gccatctgcc gctgagtccg
     8341 ggcgaagatc cggaactggg tctggaaatg acggccggct ttggtctcgg tggtctgcgt
     8401 ctgacggccc tccaagccca accgggcagc attgttccaa ccctcgttct ggcggaaagt
     8461 ctgccgggtg ccacgacccg tcaagatcaa gtggaacagt ttctggcgcg ccacaaaggt
     8521 ccgggtctcc aacatgtggg cctctacacc ccgaatattg ttgaagccac cgaaggtgtt
     8581 gcgaccgccg gtggccaatt tctggcccca ccgggcgcct actaccaaca accgggcaaa
     8641 gagcgccaaa ttcgcgcggc cggtcacgaa ccacatctgc tggcccgcca aggtattctg
     8701 ctcgatggcg acaagggcaa gtttctgctc caagttttca ccaagagtct gttcaccgaa
     8761 gacaccttct ttctggagct gatccagcgt caaggcgcca cgggttttgg ccaaggcaac
     8821 atccgtgcgc tgtggcagag tgttcaagaa cagagcgccc gtagccaaga agcccaccca
     8881 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     8941 ccgcggttcg aaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     9001 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     9061 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     9121 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc ccgctacccc
     9181 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     9241 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     9301 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     9361 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     9421 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc aca
//
