LOCUS       sequence-34526-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPVIR
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           107..228
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           256..270
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           298..424
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(482..505)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcgagcgcac cgagggccgc caccacctgt tcctgtagcg gccgcgactc tagaatttac
       61 tagtcatatg ataagatctc tcgactaggg ataacagggt aattgtttga atgaggcttc
      121 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
      181 ggttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gcaggaatta
      241 taatgcttat ctatagtgaa gccacagatg tatagataag cattataatt cctatgccta
      301 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
      361 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
      421 cttttttcaa ttctagcaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
      481 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
      541 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
      601 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
      661 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
      721 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
      781 tttctgggct c
//
