LOCUS       sequence-345264-       11487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ERBB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Braunstein EM, Li R, Sobreira N, Marosy B, Hetrick K, Doheny K, 
            Gocke CD, Valle D, Brodsky RA, Cheng L
  TITLE     A germline ERBB3 variant is a candidate for predisposition to 
            erythroid MDS/erythroleukemia.
  JOURNAL   Leukemia. 2016 Nov;30(11):2242-2245. doi: 10.1038/leu.2016.173. Epub
            2016 Jun 15.
  PUBMED    27416908
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/leu.2016.173"; journalName: "Leukemia"; date: "2016-11"; 
            volume: "30"; issue: "11"; pages: "2242-2245"
COMMENT     Sequence Label: pLX304-ERBB2
FEATURES             Location/Qualifiers
     source          1..11487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     225..245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        227..245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        273..499
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             500..680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    727..852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1345..1578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1763..1807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1982..2482
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2401..2419
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2494..2889
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2949..3066
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3183..3486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3487..3690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3641..3661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3713..3737
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7503..7527)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7529..7570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7612..8200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7665..7685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8083..8094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8272..8505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8583..8704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8620..8639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8674..8693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8744..8879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8899..8918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8900..8918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8928..8945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8928..8944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8937..8959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9086..9541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9173..9192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9383..9404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9567..9671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9672..9740,9741..10532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9890..9909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10703..11291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11192..11211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11445..11462
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      241 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
      301 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      361 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      421 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      481 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      661 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      721 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      781 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
      841 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
      901 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
      961 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1021 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1081 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1141 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1201 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1261 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1321 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1381 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1441 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1501 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1561 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1621 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1681 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1741 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1801 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1861 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     1921 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     1981 cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     2041 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     2101 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     2161 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     2221 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     2281 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     2341 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     2401 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     2461 aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa
     2521 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     2581 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     2641 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     2701 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     2761 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     2821 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     2881 tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc
     2941 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     3001 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     3061 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc
     3121 cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     3181 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3301 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3361 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3421 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3481 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     3541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     3601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     3661 gtacggtggg aggtctatat aagcagagct ctctggctaa ctgtcgggat caacaagttt
     3721 gtacaaaaaa gttggcacca tggagctggc ggccttgtgc cgctgggggc tcctcctcgc
     3781 cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca tgaagctgcg
     3841 gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc agggctgcca
     3901 ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagcc tgtccttcct
     3961 gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag tgaggcaggt
     4021 cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca actatgccct
     4081 ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag gggcctcccc
     4141 aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag gaggggtctt
     4201 gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg acatcttcca
     4261 caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg cctgccaccc
     4321 ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg attgtcagag
     4381 cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac tgcccactga
     4441 ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg actgcctggc
     4501 ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc tggtcaccta
     4561 caacacagac acgtttgagt ccatgcccaa tcccgagggc cggtatacat tcggcgccag
     4621 ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct gcaccctcgt
     4681 ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt gtgagaagtg
     4741 cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc gagaggtgag
     4801 ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct ttgggagcct
     4861 ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc cgctccagcc
     4921 agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat acatctcagc
     4981 atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa tccggggacg
     5041 aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca gctggctggg
     5101 gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata acacccacct
     5161 ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc aagctctgct
     5221 ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct gccaccagct
     5281 gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact gcagccagtt
     5341 ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc ccagggagta
     5401 tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga atggctcagt
     5461 gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata aggaccctcc
     5521 cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca tgcccatctg
     5581 gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca cccactcctg
     5641 tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc tgacgtccat
     5701 catctctgcg gtggttggca ttctgctggt cgtggtcttg ggggtggtct ttgggatcct
     5761 catcaagcga cggcagcaga agatccggaa gtacacgatg cggagactgc tgcaggaaac
     5821 ggagctggtg gagccgctga cacctagcgg agcgatgccc aaccaggcgc agatgcggat
     5881 cctgaaagag acggagctga ggaaggtgaa ggtgcttgga tctggcgctt ttggcacagt
     5941 ctacaagggc atctggatcc ctgatgggga gaatgtgaaa attccagtgg ccatcaaagt
     6001 gttgagggaa aacacatccc ccaaagccaa caaagaaatc ttagacgaag catacgtgat
     6061 ggctggtgtg ggctccccat atgtctcccg ccttctgggc atctgcctga catccacggt
     6121 gcagctggtg acacagctta tgccctatgg ctgcctctta gaccatgtcc gggaaaaccg
     6181 cggacgcctg ggctcccagg acctgctgaa ctggtgtatg cagattgcca aggggatgag
     6241 ctacctggag gatgtgcggc tcgtacacag ggacttggcc gctcggaacg tgctggtcaa
     6301 gagtcccaac catgtcaaaa ttacagactt cgggctggct cggctgctgg acattgacga
     6361 gacagagtac catgcagatg ggggcaaggt gcccatcaag tggatggcgc tggagtccat
     6421 tctccgccgg cggttcaccc accagagtga tgtgtggagt tatggtgtga ctgtgtggga
     6481 gctgatgact tttggggcca aaccttacga tgggatccca gcccgggaga tccctgacct
     6541 gctggaaaag ggggagcggc tgccccagcc ccccatctgc accattgatg tctacatgat
     6601 catggtcaaa tgttggatga ttgactctga atgtcggcca agattccggg agttggtgtc
     6661 tgaattctcc cgcatggcca gggaccccca gcgctttgtg gtcatccaga atgaggactt
     6721 gggcccagcc agtcccttgg acagcacctt ctaccgctca ctgctggagg acgatgacat
     6781 gggggacctg gtggatgctg aggagtatct ggtaccccag cagggcttct tctgtccaga
     6841 ccctgccccg ggcgctgggg gcatggtcca ccacaggcac cgcagctcat ctaccaggag
     6901 tggcggtggg gacctgacac tagggctgga gccctctgaa gaggaggccc ccaggtctcc
     6961 actggcaccc tccgaagggg ctggctccga tgtatttgat ggtgacctgg gaatgggggc
     7021 agccaagggg ctgcaaagcc tccccacaca tgaccccagc cctctacagc ggtacagtga
     7081 ggaccccaca gtacccctgc cctctgagac tgatggctac gttgcccccc tgacctgcag
     7141 cccccagcct gaatatgtga accagccaga tgttcggccc cagccccctt cgccccgaga
     7201 gggccctctg cctgctgccc gacctgctgg tgccactctg gaaaggccca agactctctc
     7261 cccagggaag aatggggtcg tcaaagacgt ttttgccttt gggggtgccg tggagaaccc
     7321 cgagtacttg acaccccagg gaggagctgc ccctcagccc caccctcctc ctgccttcag
     7381 cccagccttc gacaacctct attactggga ccaggaccca ccagagcggg gggctccacc
     7441 cagcaccttc aaagggacac ctacggcaga gaacccagag tacctgggtc tggacgtgcc
     7501 agtgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct
     7561 cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct
     7621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7981 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8161 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     8221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8281 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     8521 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8581 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8701 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     8761 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8821 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8881 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     8941 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9001 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9061 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9541 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11461 aggaagcgga agagcgccca atacgca
//
