LOCUS       sequence-345266-        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a 
            of DAF-16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH2930
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     177..196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     430..447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    564..585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(600..605,606..623,624..630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    638..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     643..665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     662..678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     662..678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2719..2747)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3495..3515
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7056..7073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7056..7072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7065..7087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7285..7740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7372..7391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7582..7603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7757..7776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7876..7898
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7936..7954)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8022..8126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8127..8195,8196..8987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8345..8364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9158..9470,1..276)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
       61 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      121 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      181 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      241 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      301 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      361 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      421 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      481 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      541 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      601 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      661 acaggaaaca gctatgacat gattacgaat tcgaatggcc atgggacgtc gacgcatagc
      721 ccgcagtcac ttgaaagcac atttaagatg gaatcaaatg gtaagaaagc tcgtgtaatc
      781 aggagatttt atccggggta tgccgaggaa tatatggtgc actcgcatta gctggcattt
      841 tttgtaatta tcggatttct acctttttct taatctagtt tgttatcatt gtccatcatt
      901 tacaaccaaa aatttgcgtc atttacaacc aaaaattgat ctgacaatta tcataattcg
      961 gtttttattt tcagaagaaa cgctccgatg tgatcctata gtgaatatac ttttccatcc
     1021 tctctgaaat aaacaattat gaagaatgca tcaatttgca ttcgaaataa ctgccgtatt
     1081 ttgtctattg gtatggctgc agtaccattt ttcttcgcgt ctttgcgcag ccttcgggcg
     1141 ctaccaataa ggctaagccg gggtaagtta tttcagaatt ctaatcaact aatctaagtc
     1201 tcatacatca ttgtcaagtg acgcattttt ttaatttcta tgttaactcg actttaatat
     1261 tcttttctgg tttcaccaga aaaatgctgg actgaaaaat tgaatttccc gcgatttggg
     1321 aaaaaccgca gtttaagctt ttggcatgaa tacagtgaac acaggaacaa aaaattgact
     1381 tgttattttg agtttatatt aaatgttaat tttgcatctc atattttcac aaaacttgtt
     1441 tgcttgaaaa atgtgcaaat aacaagtgaa catacatcaa ggtttatgca gatcagcact
     1501 gatgcaactg atagcaaaac tgataagggt caaaatttca gtggccagca caaacacata
     1561 cacattcgta gttcgttttg tgtttcgtgt atttagcatt tatttttcaa ttccggcaat
     1621 ttgccgattt gccggaaatt ttgatttttg gcaaattgcc agtttgccgt ttgccgaaca
     1681 tcaatttgcc gaaatttttt agagggatct ttataagacg gaaacactta aaattgtgct
     1741 tttttggaat ttttttcaat gtttcaatag aattagcttg catttaaaat tagatgtagg
     1801 aacgtttata ggacgcttac aattttgccg attaaaatcg aagttctgaa cttttcaaaa
     1861 aatgtgcaaa attttttttg acaaatttga caaatcggcc ggtttgtcga ttgtccggaa
     1921 tttttcagtt tcggcgattt accggtttgc cggaaaacat cgtttgccac ccacacacac
     1981 ataaagagac gaaacaacac accatcttca aaaaatttgt gaatcttcat ttccacgtgg
     2041 tttgcagggt ttcccatcca ccccactcta tctctccaag ttgaattttg gcaaaattga
     2101 ccacacaccg atcaaaaact attgtgtgtg tgtgtatttg tgtcttcaat aattcctgtc
     2161 tgtgtctcgc actatctatt gactacaaga acgtgatcgc agctctacga aacctttact
     2221 ttattttaga aatatttttt catgctatta gcaaaaaaaa agtttttttt tgtctaagta
     2281 cacttaaaat tttccaaaat ttttaagatt tttcaaaatg tgcgatcaaa gatctgacaa
     2341 aatgccaaaa ttttgaagag gaaatttagt cggcttccaa aattataagt ggcagaaatt
     2401 gagcaattgt cactttttga cagtaaatag aaaatttttt gaaaaatttt gaaacgcttt
     2461 attttgttac ttggtcattt tggcaccata ggagtagttt ttaacatttt ccccactggc
     2521 gctacttcat tttaaacttt tccaaagatt ttaattttta attttaaaaa atttcatgta
     2581 attcgtctgc gtcttacagt ttcaggcttc aaaaatctat tacatatctt atctttgaat
     2641 tcagggatcc ccgggtaccg agctcgaatt caagtaccgg tagaaaaaat gagtaaagga
     2701 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     2761 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     2821 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
     2881 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
     2941 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     3001 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     3061 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
     3121 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     3181 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     3241 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta
     3301 ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat
     3361 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     3421 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     3481 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     3541 gacgaactat acaaaagccc acaagcttac atgtcctggg cccatcgatt cggacgtccc
     3601 gagggcttaa gcggacgcgt cgaggggatc cctatgatgg agatgctggt agatcaggga
     3661 actgatgcat cgtcatccgc ctccacgtcc acctcatctg tttcgagatt cggagcggac
     3721 acgttcatga atacaccgga tgatgtgatg atgaatgatg atatggaacc gattcctcgt
     3781 gatcggtgca atacgtggcc aatgcgtagg ccgcaactcg aaccaccact caactcgagt
     3841 cccattattc atgaacaaat tcctgaagaa gatgctgacc tatacgggag caatgagcaa
     3901 tgtggacagc tcggcggagc atcttcaaac gggtcgacag caatgcttca tactccagat
     3961 ggaagcaatt ctcatcagac atcgtttcct tcggaaatgt ccgaatcgcc agacgatacc
     4021 gtatcgggaa aaaagacaac gaccagacgg aacgcttggg gaaatatgtc atatgctgaa
     4081 cttatcacta cagccattat ggctagtcca gagaaacggt taactcttgc acaagtttac
     4141 gaatggatgg tccagaatgt tccatacttc agggataagg gagattcgaa cagttcagct
     4201 ggatggaaga actcgatccg tcacaatctg tctcttcatt ctcgtttcat gcgaattcag
     4261 aatgaaggag ccggaaagag ctcgtggtgg gttattaatc cagatgcaaa gccaggaagg
     4321 aatccacggc gtacacgtga acgatccaat actattgaga cgactacaaa ggctcaactc
     4381 gaaaaatctc gccgcggagc caagaagagg ataaaggaga gagcattgat gggctccctt
     4441 cactcgacac ttaatggaaa ttcgattgcc ggatcgattc aaacgatttc tcacgatttg
     4501 tatgatgatg attcaatgca aggagcattt gataacgttc catcgtcttt ccggttagta
     4561 atttagtttt atttttattt tttgaatttt ccaaatttcc aaattttttc agtccccgaa
     4621 ctcaatcgaa cctctcgatt cctggatcgt cgtctcgtgt ttctccagct attggaagtg
     4681 atatctatga tgatctagaa ttcccatcat cggttggcga atcggttcca gcaattccaa
     4741 gtgatattgt tgatagaact gatcaaatgc gtatcgatgc aactactcat attggtggag
     4801 ttcagattaa gcaggagtcg aagccgatta aggtgagaaa tgagaatttg caattttcag
     4861 gaaaatttga aaattttctt gtggttagta caagtgagct cctttttaga caattaaaaa
     4921 atttgaattt tacttaaatt caatattttt aattctgaat ttagtttcaa ggtcccctaa
     4981 aatattagta acatgcaaat cattttatat tttctcacac cgaaaattag aattttcttt
     5041 ttttttaagt gaaaaaactg agtaatatat agaattttca gacggaacca attgctccac
     5101 caccatcata ccacgagttg aacagtgtcc gtggatcgtg tgctcagaat ccacttcttc
     5161 gaaatccaat tgtgccaagc actaacttca agccgttagt ttttttttga acagaagtta
     5221 attaatctat cgtaatttca caagattttg ggtcccggcg ctcaaaaatg ttttttttaa
     5281 atttttatgt taaaaatagt tttaaatcag ttttgaaagt gtaaattttg gcttaaaatt
     5341 attttaaaca gaaaattgga aataaaacga agaaataaac ttttttgcgc gccgagaccc
     5401 atctgaataa atgcgtgcgc ctttaagaaa cctgtgaaat tactatatat acaaaattct
     5461 gagaatgcgt attacgcaac aaatttgacg cgcaaaatat ctcgtagcga aaactacagt
     5521 aatccttaaa atgagtactg tagcgcttgc gtcgatttac gggctcgatt ttcgtgaatt
     5581 aatttatttt cgaaaagtga cagcgatatt acatttaact ttgttctttc taatcatttt
     5641 ctcatttttg tgaattttta aaaataattt ttcgattaat aaaggattgc cgtaaatcga
     5701 cacatggtgc tacagtaggc atttaaagga ttactgtagt tttcgctacg agatatttcg
     5761 cgcgtcaaat atgttgcgta atacgcattc tcagaatttt atgatcccat aatatatatt
     5821 tcttctttaa ttcaatatat aataattatc caaaatttgt tcagaatgcc actaccgggt
     5881 gcctatggaa actatcaaaa tggtggaata actccaatca attggctatc aacatccagc
     5941 tcatctccac tgcctggaat tcaatcgtgt ggaattgtag ctgcacagca tactgtcgct
     6001 tcttcatcgg ctcttccaat tgatttggaa aatctgacac ttcccgatca gccactgatg
     6061 gatactatgg atgttgatgc attgatcaga catgagctga gtcaagctgg agggcagcag
     6121 catattcatt ttgatttgta actgcaggaa ttcgatatgc agaagctgat ctcagaggag
     6181 gacctgcttc agaagcttat ctcagaggag gaccttcgac ctcgaggggg ggcccggtac
     6241 catggtattg atatctgagc tccgcatcgg ccgctgtcat cagatcgcca tctcgcgccc
     6301 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     6361 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     6421 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     6481 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     6541 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     6601 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     6661 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     6721 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     6781 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     6841 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     6901 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     6961 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttctac
     7021 tagtggccac gtgggccgtg caccttaagc ttggcactgg ccgtcgtttt acaacgtcgt
     7081 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7141 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7201 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     7261 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7321 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7381 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7441 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7501 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7561 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7621 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7681 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     7741 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     7801 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7861 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7921 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7981 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8041 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8101 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8161 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8221 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8281 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8341 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8401 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8461 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8521 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8581 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8641 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8701 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8761 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8821 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8881 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8941 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9001 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9061 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
//
