LOCUS       sequence-345288-        9009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) 
            (REPAIR.t1-S expression, specificity enhancing mutations).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Kannan S, Altae-Tran H, Jin X, Madigan VJ, Oshiro R, Makarova KS, 
            Koonin EV, Zhang F
  TITLE     Compact RNA editors with small Cas13 proteins.
  JOURNAL   Nat Biotechnol. 2021 Aug 30. pii: 10.1038/s41587-021-01030-2. doi: 
            10.1038/s41587-021-01030-2.
  PUBMED    34462587
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Aug 30. pii: 10.1038/s41587-021-01030-2. doi: 
            10.1038/s41587-021-01030-2."
COMMENT     Sequence Label: pAB1923 REPAIR.t1-S
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      683..692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             704..730
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(4366..4383)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4372..4596
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4642..5070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4729..4748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4939..4960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5079..5099)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5084..5413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5264..5399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5326..5345
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5480..6274
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5534..5553)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6144..6163
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6448..6569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6485..6504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6539..6558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6618..6634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6618..6634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6631..6653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6642..6658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6666..6672,6673..6690,6691..6696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6711..6732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6849..6866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7020..7608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7100..7119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7779..8570,8571..8639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8402..8421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8640..8744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8819..8838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgccaccat gggatccctt caactgcctc cacttgaaag
      721 actgacactg ggatccgaat tcgagaacat caagaaaacc agcaacaaag aggtgtacag
      781 catcgagcag tacgagggcg agaagaagtg gtgcttcgcc atcgtgctga acagagccca
      841 gaccaacctg gaagagaacc ccaagctgtt cgagcagacc ctgaccagat ttgagaagat
      901 catgaagcag gactggttca acgaggaaac gaagaagctg atctacgaga aagaggaaga
      961 aaacaaagtc aaagaggaaa tccagatcgc cgccagcgag cggctgaaga acctggcaaa
     1021 ctacttcagc gcctacctgc acgcccctga ctgcctgatc ttcaaccgga acgacaccat
     1081 ccggatcatc atggaaaagg cctacgagaa gtcccgcttc gaggccaaga agaagcagca
     1141 agaggacatc tccatcgagt tccccgagct gtttgaggaa gaggacaaga tcacctctgc
     1201 cggcgtggtg ttcttcgtca gcttttttat cgagcggcgg ttcctgaacc ggctgatggg
     1261 ctatgtgcag ggcttcagaa agacagaggg cgagtacaac atcaccagac aggtgttcag
     1321 caagtactgc ctgaaggaca gctacagcgt gcaggcccag gatcacgatg ccgtgatgtt
     1381 cagagacatc ctgggctacc tgagcagagt gcccaccgag atctaccagc acatcaagct
     1441 gacccggaag cggagccagg atcagctgag cgagagaaag accgacaagt tcatcctgtt
     1501 cgccctgaag tacctcgagg actacggact gaaggacctg gccgattaca ccgcctgctt
     1561 tgccagaagc aagatcaaga gagagaacga ggacaccaaa gagacagacg gcaacaagca
     1621 caagttccac cgcgagaagc ccgtggtgga aatccacttc gacaaagaga agcaggatca
     1681 gttctacatc aagcggaaca acgtcatcct gaaggcccag aagaaaggcg gccagagcaa
     1741 cgtgttccgg atgggagtgt acgagctgaa gtatctggtg ctgctgagcc tgctgggcaa
     1801 agccgaagag gccatccaga gaatcgaccg gtacatcagc agcctgaaga aacagctgcc
     1861 ctacctggat aagatcagca acgaagagat ccagaagtcc atcaactttc tgcccagatt
     1921 cgtgcggagc cggctgggac tgctgcaagt ggatgatgag aagagactga aaacccggct
     1981 ggaatacgtg aaggccaagt ggaccgataa gaaagagggc agcagaaagc tggaactgca
     2041 ccggaagggc agagatatcc tgcggtatat caacgagaga tgcgacagac ccctgagccg
     2101 gaaagagtat aacaacattc tgaagttcat cgtgaacaag gacttcgccg gcttctacaa
     2161 cgagctggaa gaactgaagc ggaccagacg gctggacaag aacatcatcc agaagctgag
     2221 cggccacacc acactgaacg ctctgcacga gcgagtgtgt gacctggtcc tgcaagagct
     2281 gggctctctg cagtccgaga acctgaaaga gtacatcgga ctgatcccca aagaagagaa
     2341 agaagtcacc ttccgcgaga aagtggacag aatcctggaa cagcctgtgg tgtacaaggg
     2401 cttcctgaga tacgagttct tcaaagaaga taagaaatcc ttcgccaggc tggtggaaga
     2461 ggctatcaag accaagtgga gcgacttcga catccctctg ggcgaagagt actacaacat
     2521 ccctagcctg gacagattcg accggaccaa caagaagctg tacgagacac tggccatgga
     2581 ccggctgtgt ctgatgatgg ccagacagta ctacctgcgg ctgaacgaga agctggccga
     2641 gaaggcacag cacatctact ggaaaaaaga ggacggccgg gaagtcatca tcttcaagtt
     2701 ccagaatccg aaagagcaga agaagtcctt cagcatcagg ttcagcatcc tggactacac
     2761 caagatgtac gtgatggacg accccgagtt cctgagcagg ctgtgggagt acttcatccc
     2821 taaagaggcc aaagagatcg actaccacaa gcactacgcc agagccttcg acaagtacac
     2881 caacctgcag aaagaaggca tcgacgccat cctgaaactg gaaggcagaa tcatcgagcg
     2941 ccggaagatc aagcccgcca agaactacat cgagtttcaa gagattatga atcggagcgg
     3001 ctacaacaac gaccagcagg ttgccctgaa gagagtggcg aacgccctgc tggcctacaa
     3061 cctgaacttc gagagagagc acctgaagcg gttctacggc gtcgtgaaga gagagggcat
     3121 cgagaaaaaa tggtccctga tcgtgggagg gtcaggcggt agtcagctgc atttaccgca
     3181 ggttttagct gacgctgtct cacgcctggt cctgggtaag tttggtgacc tgaccgacaa
     3241 cttctcctcc cctcacgctc gcagaaaagt gctggctgga gtcgtcatga caacaggcac
     3301 agatgttaaa gatgccaagg tgataagtgt ttctacagga acaaaatgta ttaatggtga
     3361 atacatgagt gatcgtggcc ttgcattaaa tgactgccat gcagaaataa tatctcggag
     3421 atccttgctc agatttcttt atacacaact tgagctttac ttaaataaca aagatgatca
     3481 aaaaagatcc atctttcaga aatcagagcg aggggggttt aggctgaagg agaatgtcca
     3541 gtttcatctg tacatcagca cctctccctg tggagatgcc agaatcttct caccacatga
     3601 gccaatcctg gaagaaccag cagatagaca cccaaatcgt aaagcaagag gacagctacg
     3661 gaccaaaata gagtctggtc aggggacgat tccagtgcgc tccaatgcga gcatccaaac
     3721 gtgggacggg gtgctgcaag gggagcggct gctcaccatg tcctgcagtg acaagattgc
     3781 acgctggaac gtggtgggca tccagggatc actgctcagc attttcgtgg agcccattta
     3841 cttctcgagc atcatcctgg gcagccttta ccacggggac cacctttcca gggccatgta
     3901 ccagcggatc tccaacatag aggacctgcc acctctctac accctcaaca agcctttgct
     3961 cagtggcatc agcaatgcag aagcacggca gccagggaag gcccccaact tcagtgtcaa
     4021 ctggacggta ggcgactccg ctattgaggt catcaacgcc acgactggga aggatggcct
     4081 gggccgcgcg tcccgcctgt gtaagcacgc gttgtactgt cgctggatgc gtgtgcacgg
     4141 caaggttccc tcccacttac tacgctccaa gattaccaag cccaacgtgt accatgagtc
     4201 caagctggcg gcaaaggagt accaggccgc caaggcgcgt ctgttcacag ccttcatcaa
     4261 ggcggggctg ggggcctggg tggagaagcc caccgagcag gacctgttct cactcacgta
     4321 agcggccgct cgagtctaga gggcccgttt aaacccactg atcagcctcg actgtgcctt
     4381 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4441 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4501 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4561 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4621 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4681 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4741 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4801 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4861 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4921 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4981 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5041 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     5101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5161 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5221 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5341 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5401 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5461 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5521 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5581 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5641 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5701 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5761 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5821 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5881 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5941 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6001 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6061 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6121 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6181 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6241 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6301 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6361 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6421 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6541 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6601 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6721 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6781 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7441 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8761 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     8821 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8881 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8941 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     9001 atacgcgtt
//
