LOCUS       sequence-34529-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtRMPVIR
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(189..206)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     416..435
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gagctgtaca agtaagaatt gttcaattcc gccccccccc cctaacgtta ctggccgaag
       61 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
      121 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
      181 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
      241 tctggaagct tcttgaaaac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
      301 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataaaata cacctgcaaa
      361 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
      421 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
      481 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtc
//
