LOCUS       sequence-34530-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtRMPVIR
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           70..191
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           219..233
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           261..387
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(445..468)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccgagggccg ccaccacctg ttcctgtagc ggccagctaa gcctcgacta gggataacag
       61 ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
      121 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct
      181 gttgacagtg agcgcaggaa ttataatgct tatctatagt gaagccacag atgtatagat
      241 aagcattata attcctatgc ctactgcctc ggaattcaag gggctacttt aggagcaatt
      301 atcttgttta ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa
      361 ttaaaatggt ataaattaaa tcactttttt caattctagc aattctaccg ggtaggggag
      421 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      481 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      541 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      601 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      661 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      721 caatagcagc tttgctcctt cgctttctgg gctca
//
