LOCUS       sequence-345336-        6166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: pB80-empty
FEATURES             Location/Qualifiers
     source          1..6166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     938..960
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1021..1030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1321..1455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1358..1377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1412..1431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1469..1485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1469..1485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1482..1504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1493..1509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1517..1523,1524..1541,1542..1547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1562..1583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1700..1717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1871..2459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1951..1970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2630..3421,3422..3490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3253..3272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3491..3595)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3663..3681
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3719..3741)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3841..3860
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4054..4076
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4068..4085
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4069..4085
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5886..6161
                     /label=chicken beta-actin promoter
ORIGIN
        1 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      241 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      301 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
      361 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
      421 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
      481 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
      541 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
      601 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
      661 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
      721 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
      781 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
      841 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgtccgcag
      901 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
      961 ggcggggttt atatcttccc ttctctgttc ctccgcagcc cccaagctag cgctaagctt
     1021 gccaccatgg gcgcgcctga attcaggatc cagtcgacta gtacttctcg agctctgtac
     1081 atgtccgcgg tcgcgacgta cgcgtatcga tggcgccagc tgcaggcggc cgccatatgc
     1141 atcctaggcc tattaatatt ccggagtata cgtagccggc taacgttaac aaccggtacc
     1201 tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta
     1261 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1441 tatcatgtct ggatcaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     1501 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1561 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1621 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1681 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1741 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1801 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1861 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1921 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1981 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2041 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2101 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2161 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2221 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2281 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2341 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2401 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2461 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2521 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2581 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2641 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2701 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2761 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2821 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2881 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2941 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3001 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3061 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3121 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3181 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3241 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3301 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3361 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3421 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3481 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3541 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3601 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3661 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     3721 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     3781 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     3841 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     3901 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3961 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     4021 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     4081 ccagtgccaa gttgggatct ttgcattggc ccacggctct caggatgggg atgctcccct
     4141 tcagcacccg gttccccttg gaaactgatg gtcctggctc tgtggcatgg cagtggcact
     4201 gtgaggagcc ccctaccagc agcacacagt ggggttggca ctgccacgct ccggatgccg
     4261 cgctctgatc caaccccata atcaagggaa cccgaattgc cccatcattg cccccaccac
     4321 ccccatcctg ccgggccctc acaccccacg ctgccttctg gtgacattcc ccagcccaaa
     4381 cccacggctt catggctacc gcggggcatt tcccattgcc gccccattat cagctctgca
     4441 cacctcccgc tgtacccatg cctcgtggct gcccttcttt gacgtataat cttctaatta
     4501 atacccggcc ttgtcaaagt ggagcacaaa cgttaattaa ttccccagca ggcaggtaat
     4561 taacagtgtg actccctttt tgctgcgagt ggggctgata cagagagatg tggcactatg
     4621 gagcccacgg ggtcctggca ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc
     4681 gcctccgagg agacagtatt gtccctgcgg tgtccctgca gctcagctct gtccacaggg
     4741 ccacctccag tttggagggg acacaatgca gccccgatgc aacccatcct cgcagcatcc
     4801 cagggacaaa gaccccactg caagaccgca cacagggctg ggtcccgctc ccctaatatc
     4861 tacagtgctt cttgcatggc cccttaatca atgcagttaa tcagcatgcg ctcatgcacc
     4921 gctctggagc tgcaaagccc ctcgcagcgc tgctcaccaa caccgcgcac cgccccggcc
     4981 cagcctgcag cacgcgctgc aaacaggaaa gaaacaaaat attgcccaaa tgtaggcaaa
     5041 ggcattcggc tgccttgacc tccgccgggc cgggccctgc ctgactcagc tccttactca
     5101 gcgctcgctt cctccctccg gctgccaccg ccgcagcgca caccctgaca aagagtggcc
     5161 cttaacgggc tctgaggtgc acccagcagt gcactcagca gtccaagggc cggcctggag
     5221 gtttgcaccg ctacgtgctg acattagcat tgaacttggc cctgggtagt gctgcaggcc
     5281 gggcggggtg ggtgtagaga gtgcagcgcg cgttgcaccc ggtgcccctt cccctccctt
     5341 gcatcccagc aggctgcacc ccagcaccag gcccgtgcat gcatgctcct ggtgttattg
     5401 cagcctggtg catgcatgcg tcttgatggt gcagcgctgt gcatgcatcc tccttggtgt
     5461 gtagcagtct agtgcatgca tacccctcgg tgttattgct gctctgtgca cgcacgctca
     5521 ttgtatcact tcatcccagt gcatgcactc acactggagc gattgctgct cggtgcacgc
     5581 acactcattg tatcactgca gctcagtggc tgcacgcaca ccggtgttat tgctgctcgg
     5641 tgcgtgcatg cacatcagtg tcgctgcagc tcagtgcatg cacgctcatt gcccatcgct
     5701 atccctgcct ctcctgctgg cgctccccgg gaggtgactt caaggggacc gcaggaccac
     5761 ctcgggggtg gggggagggc tgcacacgcg gaccccgctc cccctcccca acaaagcact
     5821 gtggaatcaa aaagggggga ggggggatgg aggggcgcgt cacacccccg ccccacaccc
     5881 tcacctcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     5941 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     6001 gggggcgcgc gccaggcggg gcggggsggg gsgrggggsg gggcggggcg aggcggagag
     6061 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     6121 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagt
//
