LOCUS       sequence-345342-        5629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-nsp12
FEATURES             Location/Qualifiers
     source          1..5629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2942..3041)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3058..3077)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3059..3077)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3082..3098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3082..3098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3527..4318
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4411..4999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4900..4919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5153..5170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5329..5356)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5448..5534)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5583..5605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5597..5614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5598..5614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcca gtgttgacat ttcttattta aaccgagcaa ggggttctag tgcagctcga
      181 ctagaaccct gcaatggcac ggacatcgat aagtgtgttc gtgcttttga catttataat
      241 aaaaatgttt cattcttggg taagtgtttg aagatgaact gtgttcgttt taaaaatgct
      301 gatcttaagg atggttattt tgttataaag aggtgtacta agtcggttat ggaacacgag
      361 caatccatgt ataacctact taacttttct ggtgctttgg ctgagcatga tttctttact
      421 tggaaagatg gcagagtcat ttatggtaat gttagtagac ataatcttac taaatatact
      481 atgatggact tggtctatgc tatgcgtaac tttgatgaac aaaattgtga tgttctaaaa
      541 gaagtattag ttttaactgg ttgttgtgac aattcttatt ttgatagtaa gggttggtat
      601 gacccagttg aaaatgaaga tatacataga gtttatgcat ctcttggcaa aattgtagct
      661 agagctatgc ttaaatgcgt tgctctatgc gatgcgatgg ttgctaaagg tgttgttggt
      721 gttttaacat tagataacca agatcttaat ggtaactttt atgattttgg tgattttgtt
      781 gttagcttac ctaatatggg tgttccctgt tgtacatcat attattctta tatgatgcct
      841 attatgggtt taactaattg tttagctagt gagtgttttg tcaagagtga tatttttggt
      901 agtgatttta aaacttttga tttgcttaag tatgatttca ctgaacataa agaaaattta
      961 ttcaataagt actttaagca ttggagtttt gattatcatc ctaattgtag tgactgttat
     1021 gatgatatgt gtgttataca ttgtgctaat tttaatacac tatttgccac aactatacca
     1081 ggtactgctt ttggtccact atgtcgtaaa gtttttatag atggtgttcc acttgttaca
     1141 actgctggtt atcattttaa gcaattaggt ttggtttgga ataaagatgt taacacacac
     1201 tcagttaggt tgacaattac tgaacttttg caatttgtca ccgacccttc cttgataata
     1261 gcttcttccc cagcactcgt tgatcaacgc actatttgtt tttctgttgc agcattgagt
     1321 actggtttga caaatcaagt tgttaagcca ggtcatttta atgaagagtt ttataacttt
     1381 cttcgtttaa gaggtttctt tgatgaaggt tctgaactta cattaaaaca tttcttcttc
     1441 gcacagaatg gtgatgctgc tgttaaagat tttgactttt accgttataa taagcctacc
     1501 attttagata tttgtcaagc tagagttaca tataagatag tctctcgtta ttttgacatt
     1561 tatgaaggtg gctgtattaa ggcatgtgaa gttgttgtaa caaatcttaa taagagtgct
     1621 ggttggccat taaataagtt tggtaaagct agtttgtatt atgaatctat atcttatgaa
     1681 gaacaggatg ctttgtttgc tttgacaaag cgtaatgtcc tccctactat gacacagctg
     1741 aatcttaagt atgctattag tggtaaagaa cgtgctagaa ctgttggtgg tgtttctctg
     1801 ttgtctacaa tgaccacaag acaataccat caaaaacatc ttaaatccat tgttaataca
     1861 cgcaatgcca ctgttgttat tggtactacc aaattttatg gtggttggaa taatatgttg
     1921 cgtactttaa ttgatggtgt tgaaaaccct atgcttatgg gttgggatta tcccaaatgt
     1981 gatagagctt tgcctaacat gatacgtatg atttcagcca tggtgttggg ctctaagcat
     2041 gttaattgtt gtactgcaac agataggttt tataggcttg gtaatgagtt ggcacaagtt
     2101 ttaacagaag ttgtttattc taatggtggt ttttatttta agccaggtgg tacgacttct
     2161 ggtgacgcta gtacagctta tgctaattct atttttaaca tttttcaagc cgtgagttct
     2221 aacattaaca ggttgcttag tgtcccatca gattcatgta ataatgttaa tgttagggat
     2281 ctacaacgac gtctgtatga taattgttat aggttaacta gtgttgaaga gtcattcatt
     2341 gatgattatt atggttatct taggaaacat ttttcaatga tgattctctc tgatgacggt
     2401 gttgtctgtt ataacaagga ttatgctgag ttaggttata tagcagacat tagtgctttt
     2461 aaagccactt tgtattacca gaataatgtc tttatgagta cttctaaatg ttgggttgaa
     2521 gaagatttaa ctaagggacc acatgagttt tgttcccagc atactatgca aatagttgac
     2581 aaagatggta cctattattt gccttaccca gatcctagta ggatcttgtc agctggtgtt
     2641 tttgttgatg atgttgttaa gacagatgct gttgttttgt tagaacgtta tgtgtcttta
     2701 gctattgatg cataccctct ttcaaaacac cctaattccg aatatcgtaa ggttttttac
     2761 gtattacttg attgggttaa gcatcttaac aaaaatttga atgagggtgt tcttgaatct
     2821 ttttctgtta cacttcttga taatcaagaa gataagtttt ggtgtgaaga tttttatgct
     2881 agtatgtatg aaaattctac aatattgcaa gcttaattaa gacgcgatcg cggcctcatg
     2941 ggcccagctt tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc
     3001 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     3061 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     3121 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
     3181 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     3241 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     3301 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     3361 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatctcg
     3421 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     3481 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     3541 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3601 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3661 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3721 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3781 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3841 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3901 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3961 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     4021 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     4081 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4141 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4201 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     4261 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     4321 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     4381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5101 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5161 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5281 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     5341 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     5401 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     5461 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     5521 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     5581 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
