LOCUS       sequence-345347-       12019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for PKG #7, a genetically encoded FRET 
            biosensor for monitoring cGMP concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Hirota K, Hirashima T, Horikawa K, Yasoda A, Matsuda M
  TITLE     C-type Natriuretic Peptide-induced PKA Activation Promotes 
            Endochondral Bone Formation in Hypertrophic Chondrocytes.
  JOURNAL   Endocrinology. 2022 Mar 1;163(3). pii: 6511000. doi: 
            10.1210/endocr/bqac005.
  PUBMED    35041746
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology."; date: "2022-03-1"; volume: "163(3). pii: 6511000. 
            doi"; pages: " 10.1210/endocr/bqac005"
COMMENT     Sequence Label: pCSIIbsr-PKG-hNo.7
FEATURES             Location/Qualifiers
     source          1..12019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1442..1675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1860..1904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2178..2295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2351..3513
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2582..3504
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3461..3481
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3542..4279
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MVDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYITADKQKNGIKANFKIRHNIE"
     primer_bind     3682..3703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3806..3827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4067..4086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4700..5413
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4730..4758)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5353..5373
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(5451..6025,6026..6028,6029..6037)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5618..5635)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5845..5864
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6038..6436
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6480..7068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6533..6553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6951..6962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7587..7767
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7793..7810)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7799..8023
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8069..8497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8156..8175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8366..8387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8506..8526)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8511..8840
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8691..8826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8753..8772
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8888..8935
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8954..9328
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9458..9579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9495..9514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9549..9568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9628..9644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9628..9644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9641..9663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9652..9668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9676..9682,9683..9700,9701..9706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9721..9742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9859..9876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10030..10618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10110..10129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10789..11580,11581..11649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11412..11431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11650..11754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11829..11848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagggc cgctgatctt cagacctgga ggaggagata
     1321 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1381 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1441 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1501 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1561 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1621 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1681 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1741 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1801 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1861 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1921 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1981 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2041 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2101 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctacaaatg
     2161 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2221 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2281 aaaattcaaa attttcgggt ttattacagg gacagcagaa attcactttg aattaattca
     2341 agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2401 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2581 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2641 gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg
     2701 ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2761 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2821 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2881 agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc
     2941 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3001 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3061 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3121 tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg
     3181 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca
     3241 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3301 tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3361 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3421 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3481 caaagttttt ttcttccatt tcaggtgtcg tgaacacgct accggtctcg acgaattcac
     3541 catggtggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3601 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     3661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3721 tctcggcatg gacgagctgt acaagggtgg cagcggtggc atggtgagca agggcgagga
     3781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct
     3901 gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta
     3961 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4021 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4201 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     4261 gatccgccac aacatcgagg gcatgcatga gtatatggaa tttttaaaaa gcgttccaac
     4321 attccagagc cttcctgaag agatcctcag caagcttgct gatgtccttg aagagaccca
     4381 ctatgaaaat ggagaatata ttatcaggca aggtgcaaga ggggacacct tctttatcat
     4441 cagcaaagga acggtaaatg tcactcgtga agactcaccg agtgaagacc cagtctttct
     4501 tagaacttta ggaaaaggag actggtttgg agagaaagcc ttgcaggggg aagatgtgag
     4561 aacagcaaac gtaattgctg cagaagctgt aacctgcctt gtgattgaca gagactcttt
     4621 taaacatttg attggagggc tggatgatgt ttctaataaa gcatatgaag atgcagaagc
     4681 taaagcaaaa tatgagctca tgagtaaagg agaagaactt ttcactggag ttgtcccaat
     4741 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     4801 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     4861 tgttccatgg ccaacacttg tcactacttt aacatggggt gttcaatgct tttcaagata
     4921 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     4981 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     5041 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     5101 aaacattctt ggacacaaat tggaatacaa ctatatttca cacaatgtat acatcacagc
     5161 agacaaacaa aagaatggaa tcaaagcaaa cttcaaaatt agacacaaca ttgaagatgg
     5221 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     5281 tttaccagac aaccattacc tgtccacaca atctgcactt tcgaaagatc ccaacgaaaa
     5341 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac taggcatgga
     5401 tgaactatac aaataactcg agggcggcgg ccgcggctct agaggatccg cccctctccc
     5461 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5521 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5581 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5641 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5701 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5761 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5821 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5881 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt
     5941 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6001 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gccaagcctt tgtctcaaga
     6061 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     6121 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     6181 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     6241 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     6301 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     6361 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     6421 tgtgtgggag ggctaagtcg agcctctaga gttaacatcg agggatcaag cttatcgata
     6481 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6541 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6601 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6661 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6721 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6781 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6841 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6901 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6961 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7021 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     7081 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7141 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7261 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7321 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     7381 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     7441 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     7501 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     7561 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7741 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     7801 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7861 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7921 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7981 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8041 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8101 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8161 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8221 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8281 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8341 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8401 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8461 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     8521 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8581 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8641 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8761 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8821 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     8881 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     8941 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9001 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9061 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9121 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9181 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9241 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9301 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9361 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9421 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9601 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11761 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    11821 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    11881 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    11941 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    12001 cgggccagat atacgcgtt
//
