LOCUS       sequence-345351-       13397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puromycin resistant lentiviral vector for expression of PAK5 
            K478R/479R D421N; PAK7 Kinase dead.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
  TITLE     Melanoma-associated mutants within the serine-rich domain of PAK5 
            direct kinase activity to mitogenic pathways.
  JOURNAL   Oncotarget. 2018 May 22;9(39):25386-25401. doi: 
            10.18632/oncotarget.25356. eCollection 2018 May 22.
  PUBMED    29875996
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25356"; journalName: "Oncotarget"; date: 
            "2018-05-22- 22"; volume: "9"; issue: "39"; pages: "25386-25401"
COMMENT     Sequence Label: pLenti-3xFLAG-Puro PAK5 K478R/479R D421N
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             213..846
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    893..1018
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1515..1748
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1933..1977
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2275..2392
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2457..2831)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2828..2837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2841..2859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2851..2898)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     3916..3935
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4095..4160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6386..6959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6554..6571)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6781..6800
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6962..7564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7461..7481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7580..8168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7633..7653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8051..8062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8375..8608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8709..8726)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8715..8939
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8985..9413
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9072..9091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9282..9303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9422..9442)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9427..9756
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9607..9742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9669..9688
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10964..11085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11134..11150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11134..11150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11147..11169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11158..11174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11182..11188,11189..11206,11207..11212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11227..11248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11365..11382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11536..12124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11616..11635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12295..13086,13087..13155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12918..12937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13156..13260)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    join(13308..13397,1..45)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
       61 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
      121 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
      181 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
      241 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
      301 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
      361 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
      421 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
      481 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
      541 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
      601 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
      661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      841 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      901 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      961 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     1021 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1081 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1141 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1201 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     1261 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     1321 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     1381 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1441 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1501 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1561 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1621 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1681 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1741 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1801 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1861 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1921 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1981 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2041 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2101 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2161 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2221 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2281 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2341 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2401 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagt ccggaatcag
     2461 tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc
     2521 cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc
     2581 gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag
     2641 gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg
     2701 tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg
     2761 gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc
     2821 aacttggcca tggtggccct cctatagtga gtcgtattat actatgccga tatactatgc
     2881 cgatgattaa ttgtcaacac gtgctgcagg tccgaggttc taggctgcca cgtcagacga
     2941 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3001 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3061 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3121 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3181 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3241 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3301 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3361 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3421 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3481 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3541 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3601 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3661 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3721 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3781 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3841 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     3901 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     3961 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4021 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttg ttgttagacg
     4081 ctagcggatc catggactac aaagaccatg acggtgatta taaagatcat gatatcgatt
     4141 acaaggatga cgatgacaag ctagcgatgt ttgggaagaa aaagaaaaag attgaaatat
     4201 ctggcccgtc caactttgaa cacagggttc atactgggtt tgatccacaa gagcagaagt
     4261 ttaccggcct tccccagcag tggcacagcc tgttagcaga tacggccaac aggccaaagc
     4321 ctatggtgga cccttcatgc atcacaccca tccagctggc tcctatgaag acaatcgtta
     4381 gaggaaacaa accctgcaag gaaacctcca tcaacggcct gctagaggat tttgacaaca
     4441 tctcggtgac tcgctccaac tccctaagga aagaaagccc acccacccca gatcagggag
     4501 cctccagcca cggtccaggc cacgcggaag aaaatggctt catcaccttc tcccagtatt
     4561 ccagcgaatc cgatactact gctgactaca cgaccgaaaa gtacagggag aagagtctct
     4621 atggagatga tctggatccg tattatagag gcagccacgc agccaagcaa aatgggcacg
     4681 taatgaaaat gaagcacggg gaggcctact attctgaggt gaagcctttg aaatccgatt
     4741 ttgccagatt ttctgccgat tatcactcac atttggactc actgagcaaa ccaagtgaat
     4801 acagtgacct caagtgggag tatcagagag cctcgagtag ctcccctctg gattattcat
     4861 tccaattcac accttctaga actgcaggga ccagcgggtg ctccaaggag agcctggcgt
     4921 acagtgaaag tgaatgggga cccagcctgg atgactatga caggaggcca aagtcttcgt
     4981 acctgaatca gacaagccct cagcccacca tgcggcagag gtccaggtca ggctcgggac
     5041 tccaggaacc gatgatgcca tttggagcaa gtgcatttaa aacccatccc caaggacact
     5101 cctacaactc ctacacctac cctcgcttgt ccgagcccac aatgtgcatt ccaaaggtgg
     5161 attacgatcg agcacagatg gtcctcagcc ctccactgtc agggtctgac acctacccca
     5221 ggggccctgc caaactacct caaagtcaaa gcaaatcggg ctattcctca agcagtcacc
     5281 agtacccgtc tgggtaccac aaagccacct tgtaccatca cccctccctg cagagcagtt
     5341 cgcagtacat ctccacggct tcctacctga gctccctcag cctctcatcc agcacctacc
     5401 cgccgcccag ctggggctcc tcctccaacc agcagccctc cagggtgtcc catgaacagt
     5461 ttcgggcggc cctgcagctg gtggtcagcc caggagaccc cagggaatac ttggccaact
     5521 ttatcaaaat cggggaaggc tcaaccggca tcgtatgcat cgccaccgag aaacacacag
     5581 ggaaacaagt tgcagtgagg agaatggacc tccggaagca acagagacga gaactgcttt
     5641 tcaatgaggt cgtgatcatg cgggattacc accatgacaa tgtggttgac atgtacagca
     5701 gctaccttgt cggcgatgag ctctgggtgg tcatggagtt tctagaaggt ggtgccttga
     5761 cagacattgt gactcacacc agaatgaatg aagaacagat agctactgtc tgcctgtcag
     5821 ttctgagagc tctctcctac cttcataacc aaggagtgat tcacagggac ataaaaagtg
     5881 actccatcct cctgacaagc gatggccgga taaagttgtc tgattttggt ttctgtgctc
     5941 aagtttccaa agaggtgccg aagaggaaat cattggttgg cactccctac tggatggccc
     6001 ctgaggtgat ttctaggcta ccttatggga cagaggtgga catctggtcc ctcgggatca
     6061 tggtgataga aatgattgat ggcgagcccc cctacttcaa tgagcctccc ctccaggcga
     6121 tgcggaggat ccgggacagt ttacctccaa gagtgaagga cctacacaag gtttcttcag
     6181 tgctccgggg attcctagac ttgatgttgg tgagggagcc ctctcagaga gcaacagccc
     6241 aggaactcct cggacatcca ttcttaaaac tagcaggtcc accgtcttgc attgtccccc
     6301 tcatgagaca atacaggcat cactgaattc tgcagatatc cagcacagtg gcggccgctc
     6361 gagtctagag ttaacgcaaa ttccgcccct ctccctcccc ccccccctaa cgttactggc
     6421 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6481 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6541 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6601 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6661 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6721 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6781 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6841 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6901 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
     6961 catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     7021 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     7081 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7141 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7201 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag
     7261 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7321 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7381 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt
     7441 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7501 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7561 ctgagttcgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7621 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7681 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7741 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7801 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7861 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7921 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7981 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8041 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8101 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8161 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     8221 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8281 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8341 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     8401 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     8461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8581 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     8641 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg tttaaacccg
     8701 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8761 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8821 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8881 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8941 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     9001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9061 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9121 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9181 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9301 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9361 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     9421 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9481 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9601 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9661 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     9721 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     9781 attttcggat ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     9841 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     9901 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     9961 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
    10021 ccgattccgg aagtgcttga cattggggaa ttaattcagc gagagcctga cctattgcat
    10081 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
    10141 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
    10201 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
    10261 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
    10321 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
    10381 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
    10441 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
    10501 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
    10561 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
    10621 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
    10681 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
    10741 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
    10801 acgccccagc actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac
    10861 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10921 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    10981 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    11101 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    11161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11941 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    12001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    13021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13261 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
    13321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    13381 tttcactgca ttctagt
//
