LOCUS       sequence-345359-        7541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-nsp3
FEATURES             Location/Qualifiers
     source          1..7541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4852..4951)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4968..4987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4969..4987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4992..5008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4992..5008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5439..6230
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6323..6911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6812..6831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7065..7082
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7241..7268)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7360..7446)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     7495..7517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7509..7526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7510..7526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg gtaaaatatc tttttctgat gatgttatag ttcatgatgt tgaacctacc
      181 cataaagtca agctcatatt tgagtttgaa gatgatgttg ttaccagtct ttgtaagaag
      241 agttttggta agtccattat ttatacaggt gattgggaag gtctacatga agttcttaca
      301 tctgcaatga atgtcattgg gcaacatatt aagttgccac aattttatat ttatgatgaa
      361 gagggtggtt atgatgtttc taaaccagtt atgatttcac aatggcctat tagtaatgat
      421 agtaatggtt gtgttgttga agcgagcact gattttcatc aattagaatg tattgttgat
      481 gactctgtta gagaagaggt tgatataatt gaacaacctt ttgaagaagt tgaacatgtg
      541 ctctcaatta agcaaccttt ttctttttct tttagagatg aattgggtgt tcgtgtttta
      601 gatcaatctg ataataattg ttggattagt accacacttg tacagttgca acttacaaag
      661 cttttggatg attctattga gatgcaattg tttaaagttg gtaaagttga ttcaattgtc
      721 caaaagtgtt atgagttgtc tcatttaatt agtggttcac ttggtgatag tggtaaactt
      781 cttagtgaac ttcttaaaga aaaatataca tgttctataa cttttgagat gtcttgtgat
      841 tgtggtaaaa agtttgatga tcaggttggt tgtttgtttt ggattatgcc ttacacaaaa
      901 ctttttcaaa aaggtgagtg ttgtatttgt cataaaatgc agacttataa gcttgttagt
      961 atgaaaggta ctggtgtgtt tgtacaggac ccagcaccta ttgacattga tgctttccct
     1021 gtgaaaccta tatgttcatc tgtatattta ggtgttaagg gttctggtca ttatcaaaca
     1081 aatttataca gttttaacaa agctattgat ggttttggtg tctttgacat taaaaatagt
     1141 agtgttaata ctgtttgttt tgttgatgtt gattttcata gtgtagaaat agaagctggt
     1201 gaagttaaac cttttgctgt atataaaaat gttaaatttt atttaggtga tatttcacac
     1261 cttgtaaact gtgtttcttt tgactttgtt gtcaatgctg ctaatgaaaa tctcttgcat
     1321 ggaggcggtg ttgcacgtgc tattgatatt ttgactgaag gtcaacttca gtcattatct
     1381 aaagattaca ttagtagtaa tggtccactt aaggttggag caggtgttat gttggagtgt
     1441 gaaaaattca acgtatttaa tgttgttggt ccgcgaactg gtaaacatga gcattcatta
     1501 cttgttgaag cttataattc tattttattt gaaaatggta ttccacttat gcctcttctt
     1561 agttgtggta tttttggtgt aaggattgaa aattctctta aagctttgtt tagttgtgac
     1621 attaataaac cattgcaagt ttttgtttat tcttcaaatg aagaacaagc tgttcttaag
     1681 tttttagatg gtttagattt aacaccagtc attgatgatg ttgatgttgt taaacctttt
     1741 agagttgaag gtaatttttc attctttgat tgtggtgtca atgccttgga tggtgatatt
     1801 tacttattat ttactaactc tattttaatg ttggataaac aaggacaatt attggacaca
     1861 aaacttaatg gtattttgca acaggcagct cttgattatc ttgctacagt taaaactgta
     1921 ccagctggta atttggttaa actttttgtt gagagttgta ccatttatat gtgtgttgta
     1981 ccatcgataa atgatctttc ttttgataaa aatcttggtc gttgtgtgcg taaacttaat
     2041 agattgaaaa cttgtgttat tgccaatgtt cctgctattg atgttttgaa aaagcttctt
     2101 tcaagtttga ctttaactgt taaatttgtt gtagagagta atgttatgga tgttaacgac
     2161 tgttttaaga atgataatgt agttttgaaa attactgaag atggtattaa tgttaaagat
     2221 gttgttgttg agtcttctaa gtcacttggt aaacaattgg gtgttgtgag tgatggtgtt
     2281 gactcttttg aaggtgtttt acctattaat actgatactg tcttatctgt agctccagaa
     2341 gttgactggg ttgcttttta cggttttgaa aaggcagcac tttttgcttc tttggatgta
     2401 aagccatatg gttaccctaa tgattttgtt ggtggtttta gagttcttgg gaccaccgac
     2461 aataattgtt gggttaatgc aacttgtata attttacagt atcttaagcc tacttttaaa
     2521 tctaagggtt taaatgttct ttggaacaaa tttgttacag gtgatgttgg accttttgtt
     2581 agttttattt attttataac tatgtcttca aagggtcaaa agggtgatgc tgaagaggca
     2641 ttatctaaat tgtcagagta tttgattagt gattctattg ttactcttga acaatattca
     2701 acttgtgaca tttgtaaaag tactgtagtt gaagttaaaa gtgctattgt ctgtgctagt
     2761 gtgcttaaag atggttgtga tgttggtttt tgtccacaca gacataaatt gcgttcacgt
     2821 gttaagtttg ttaatggacg tgttgttatt accaatgttg gtgaacctat aatttcacaa
     2881 ccttctaagt tgcttaatgg tattgcttat acaacatttt caggttcttt tgataacggt
     2941 cactatgtag tttatgatgc tgctaataat gctgtctatg atggtgctcg tttattttct
     3001 tcagatttgt ctactttagc tgttacagct attgttgtag taggtggttg tgtaacatct
     3061 aatgttccaa caattgttag tgagaaaatt tctgttatgg ataaacttga tactggtgca
     3121 caaaaatttt tccaatttgg tgattttgtt atgaataaca ttgttctgtt tttaacttgg
     3181 ttgcttagta tgtttagtct tttacgtact tctattatga agcatgatat taaagttatt
     3241 gccaaggctc ctaaacgtac aggtgttatt ttgacacgta gttttaagta taacattaga
     3301 tctgctttgt ttgttataaa gcagaagtgg tgtgttattg ttactttgtt taagttctta
     3361 ttattattat atgctattta tgcacttgtt tttatgattg tgcaatttag tccttttaat
     3421 agtcttttat gtggtgacat tgtaagtggt tatgaaaaat ccacttttaa taaggatatt
     3481 tattgtggta attctatggt ttgtaagatg tgtttgttca gttatcaaga gtttaatgat
     3541 ttggatcata ctagtcttgt ttggaagcac attcgtgatc ctatattaat cagtttacaa
     3601 ccatttgtta tacttgttat tttgttaatt tttggtaata tgtatttgcg ttttggactt
     3661 ttatattttg ttgcacaatt tattagtact tttggttctt tcttaggctt tcatcagaaa
     3721 cagtggtttt tacattttgt gccgtttgat gttttatgta atgagttttt agctacattt
     3781 attgtctgca aaatcgtttt atttgttaga catattattg ttggctgtaa taatgctgac
     3841 tgtgtagctt gttctaaaag tgctagactt aaacgtgtac cacttcaaac tattattaat
     3901 ggtatgcata aatcattcta tgttaatgct aatggtggta cttgtttctg taataaacat
     3961 aacttctttt gtgttaattg tgattctttt gggcctggta atacttttat taatggtgat
     4021 attgcaagag agcttggtaa tgttgttaaa acagctgttc aacccacagc tcctgcatat
     4081 gttattattg ataaggtaga ttttgttaat ggattttatc gtctttatag tggtgacact
     4141 ttttggcggt atgactttga cattactgaa tctaagtata gttgtaaaga ggttctgaag
     4201 aattgtaatg ttttagaaaa ttttattgtt tacaataata gtggtagtaa cattacacag
     4261 attaaaaatg cttgtgttta tttttctcaa ttgttgtgtg aacctataaa gttggtaaat
     4321 tcagagttgt tgtcaacttt atctgttgat tttaatggtg ttttgcataa ggcatatgtt
     4381 gatgttttgt gtaatagttt ttttaaggag ttaactgcta acatgtccat ggctgaatgt
     4441 aaagctacac ttggtttgac tgtttctgat gatgattttg tttcagctgt tgccaatgca
     4501 cataggtatg acgttttgct ttcagatttg tcatttaata atttttttat ttcttatgct
     4561 aaacctgaag ataagttgtc cgtttatgac attgcttgtt gtatgcgtgc cggttctaag
     4621 gttgttaacc ataatgtttt gatcaaagag tcaataccta ttgtttgggg tgtcaaggac
     4681 tttaatactc tttctcaaga aggtaagaag taccttgtta aaacaactaa agcaaagggt
     4741 ttgacttttt tattaacttt taatgataac caagcaatta cacaagttcc tgctactagt
     4801 atagttgcaa aacagggtgc tcttaattaa gacgcgatcg cggcctcatg ggcccagctt
     4861 tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc aacgaacagg
     4921 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     4981 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     5041 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     5101 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     5161 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     5221 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     5281 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     5341 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     5401 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     5461 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     5521 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     5581 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     5641 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     5701 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     5761 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     5821 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     5881 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     5941 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     6001 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     6061 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     6121 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     6181 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     6241 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7201 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     7261 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     7321 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     7381 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     7441 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     7501 cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
