LOCUS       sequence-345380-        3351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneVIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb8
FEATURES             Location/Qualifiers
     source          1..3351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     40..105
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     119..135
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     120..136
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     374..439
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     complement(500..519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(501..519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(526..543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(526..542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(535..557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(684..1139)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(821..842)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1033..1052
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1166..1270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1271..1339,1340..2131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1489..1508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2302..2890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2791..2810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3044..3061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3178..3199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3214..3219,3220..3237,3238..3244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3252..3268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3257..3279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3276..3292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3276..3292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3313..3331
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtggcggcc gcaaattcta tttcaaggag acagtcataa tgaaatacct attgcctacg
       61 gcagccgctg gattgttatt actcgctgcc caaccagcca tggcccaggt gaaactgctc
      121 gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt
      181 gctgagggtg acgatcccgc aaaagcggcc tttaactccc tgcaagcctc agcgaccgaa
      241 tatatcggtt atgcgtgggc gatggttgtt gtcattgtcg gcgcaactat cggtatcaag
      301 ctgtttaaga aattcacctc gaaagcaagc tgatagaatt ctaaactagc tagtcgccaa
      361 ggagacagtc ataatgaaat acctattgcc tacggcagcc gctggattgt tattactcgc
      421 tgcccaacca gccatggccg agctcgtcag ttctagagtt aagcggccgc aatcgagggg
      481 gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
      541 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      601 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      661 gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat attttgttaa aattcgcgtt
      721 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      781 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      841 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      901 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      961 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1021 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1081 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1141 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1261 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1321 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1381 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1441 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1501 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1561 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1621 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1681 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1741 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1801 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1861 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1921 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1981 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2041 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2101 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2161 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2221 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2281 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2341 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2401 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2461 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2521 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2581 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2641 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2701 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2761 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2821 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2881 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2941 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3001 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3061 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3121 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3181 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     3241 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3301 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggccaccg c
//
