LOCUS       sequence-345382-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression human RAP containing a C-terminal 6xHis tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Bang YJ, Hu Z, Li Y, Gattu S, Ruhn KA, Raj P, Herz J, Hooper LV
  TITLE     Serum amyloid A delivers retinol to intestinal myeloid cells to 
            promote adaptive immunity.
  JOURNAL   Science. 2021 Sep 17;373(6561):eabf9232. doi: 
            10.1126/science.abf9232. Epub 2021 Sep 17.
  PUBMED    34529485
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-09-17"; volume: "373(6561):eabf9232. doi"; pages: " 
            10.1126/science.abf9232. Epub 2021 Sep 17"
COMMENT     Sequence Label: hRAP-pEZT-BM
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1072..1089
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcgccgc ggagggtcag gtcgtttctg cgcgggctcc cggcgctgct actgctgctg
       61 ctcttcctcg ggccctggcc cgctgcgagc cacggcggca agtactcgcg ggagaagaac
      121 cagcccaagc cgtccccgaa acgcgagtcc ggagaggagt tccgcatgga gaagttgaac
      181 cagctgtggg agaaggccca gcgactgcat cttcctcccg tgaggctggc cgagctccac
      241 gctgatctga agatacagga gagggacgaa ctcgcctgga agaaactaaa gcttgacggc
      301 ttggacgaag atggggagaa ggaagcgaga ctcatacgca acctcaatgt catcttggcc
      361 aagtatggtc tggacggaaa gaaggacgct cggcaggtga ccagcaactc cctcagtggc
      421 acccaggaag acgggctgga tgaccccagg ctggaaaagc tgtggcacaa ggcgaagacc
      481 tctgggaaat tctccggcga agaactggac aagctctggc gggagttcct gcatcacaaa
      541 gagaaagttc acgagtacaa cgtcctgctg gagaccctga gcaggaccga agaaatccac
      601 gagaacgtca ttagcccctc ggacctgagc gacatcaagg gcagcgtcct gcacagcagg
      661 cacacggagc tgaaggagaa gctgcgcagc atcaaccagg gcctggaccg cctgcgcagg
      721 gtcagccacc agggctacag cactgaggct gagttcgagg agcccagggt gattgacctg
      781 tgggacctgg cgcagtccgc caacctcacg gacaaggagc tggaggcgtt ccgggaggag
      841 ctcaagcact tcgaagccaa aatcgagaag cacaaccact accagaagca gctggagatt
      901 gcgcacgaga agctgaggca cgcagagagc gtgggcgacg gcgagcgtgt gagccgcagc
      961 cgcgagaagc acgccctgct ggaggggcgg accaaggagc tgggctacac ggtgaagaag
     1021 catctgcagg acctgtccgg caggatctcc agagctcggc acaacgaact ccatcatcac
     1081 catcaccatt ga
//
