LOCUS       sequence-34539-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Frigeri C, Tsao J, Czerwinski W, Schimmer BP
  TITLE     Impaired steroidogenic factor 1 (NR5A1) activity in mutant Y1 mouse 
            adrenocortical tumor cells.
  JOURNAL   Mol Endocrinol. 2000 Apr . 14(4):535-44.
  PUBMED    10770490
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol. 2000 Apr . 14(4):535-44."
COMMENT     Sequence Label: His-tagged-SF1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..162
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(166..174,175..189)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgtt taaacttaag cttaccatgg ggggttctca tcatcatcat
      121 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      181 gacgataagg taccaggatc cagtgtggtg gaattctcct tccgttcagc ggacgccgcg
      241 ggcatggact attcgtacga cgaggacctg gacgagctgt gtccagtgtg tggtgacaag
      301 gtgtcgggct accactacgg gctgctcacg tgcgagagct gcaagggctt cttcaagcgc
      361 acagtccaga acaacaagca ttacacgtgc accgagagtc agagctgcaa aatcgacaag
      421 acgcagcgta agcgctgtcc cttctgccgc ttccagaagt gcctgacggt gggcatgcgc
      481 ctggaagctg tgcgtgctga tcgaatgcgg ggtggccgga acaagtttgg gcccatgtac
      541 aagagagacc gggccttgaa gcagcagaag aaagcacaga ttcgggccaa tggcttcaag
      601 ctggagaccg gaccaccgat gggggtgccc ccgccacccc ctcccccacc ggactacatg
      661 ttacccccta gcctgcacgc accggagccc aaggccctgg tctctggccc acccagtggg
      721 ccgctgggtg actttggagc cccatctcta cccatggctg tgcctggtcc ccacggacct
      781 ctggctggct acctctatcc tgccttctct aaccgcacca tcaagtctga gtatccagag
      841 ccctatgcca gccccccaca acagccaggg gccaccctac agctatccag agcccttctc
      901 ag
//
