LOCUS       sequence-34540-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Frigeri C, Tsao J, Czerwinski W, Schimmer BP
  TITLE     Impaired steroidogenic factor 1 (NR5A1) activity in mutant Y1 mouse 
            adrenocortical tumor cells.
  JOURNAL   Mol Endocrinol. 2000 Apr . 14(4):535-44.
  PUBMED    10770490
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol. 2000 Apr . 14(4):535-44."
COMMENT     Sequence Label: His-tagged-SF1 (antisense)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             122..139
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             143..175
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(179..187,188..202)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttacca tggggggttc
      121 tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga
      181 tctgtacgac gatgacgata aggtaccagg atccagtgtg gtggaattcc ggctacacca
      241 gactcctcct ccctgggtag ccgggccctg ggagaaggat gatcacacca aagtcctcct
      301 acaggtagag gatcagaatt agatgggtag agggcggaaa gggctctttt tgccctaccc
      361 actctcatgg tatcctctgg ggacccaatt tagcaaactc agtttacaac agtggacttc
      421 ctgcttcagt ggagtctgga gaccccctcc tttgccctca ttgatgcctg tagaccttcc
      481 tttgcctctg ataacaggag agagggaaag gatgacctct gacccaaggg acatccagac
      541 ccaccaggga atcctgagcc tcctcctggg acctcagata ccaggaaaaa aagctggggg
      601 ctgatggagg aaggaatggt tagaagcccc ctttggggca ctccaagtgg cggacacagg
      661 ccaatggcta gtaaaggcaa cctgccccgg ccaatccccg cccatccttg cccaaacaca
      721 cagctctgga gctcaggggc aggggcttag tgacagggct cctggatcac ctaatgcaag
      781 gagtcttttg gaggcagtgg catccctgcc tattgcccgc tgcctggcac ccaggctcaa
      841 gtctgcttgg cctgcagcat ctcaatg
//
