LOCUS       sequence-345401-        4521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A7_STOP
FEATURES             Location/Qualifiers
     source          1..4521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2078..2177)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2194..2213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2195..2213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2218..2234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2218..2234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2336..2355)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2347..3156
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2414..2433)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3303..3891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3792..3811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4045..4062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4221..4248)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4340..4426)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4475..4497
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4489..4506
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4490..4506
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cggcgccaag
      121 cggaagaaga agagcatgct gtggtccaag atgcacacac cacagtgcga ggacatcatc
      181 cagtggtgtc ggagaaggct gcccatcctg gattgggccc ctcactacaa tctgaaggag
      241 aacctgctgc ccgacaccgt gtctggcatc atgctggcag tgcagcaggt gacacaggga
      301 ctggccttcg ccgtgctgag ctccgtgcac cccgtgttcg gcctgtatgg cagcctgttc
      361 ccagccatca tctacgccat ctttggaatg ggacaccacg tggcaaccgg cacattcgcc
      421 ctgacctccc tgatctctgc caatgccgtg gagcgcatcg tgccccagaa tatgcagaac
      481 ctgaccacac agagcaacac atccgtgctg ggcctgtccg attttgagat gcagaggatc
      541 cacgtggcag cagccgtgtc tttcctggga ggcgtgatcc aggtggccat gtttgtgctg
      601 cagctgggct ctgccacctt cgtggtgaca gagcctgtga tcagcgccat gaccacagga
      661 gcagcaaccc acgtggtgac aagccaggtg aagtatctgc tgggcatgaa gatgccttac
      721 atctccggcc cactgggctt cttttacatc tatgcctacg tgtttgagaa tatcaagagc
      781 gtgagactgg aggccctgct gctgtctctg ctgagcatcg tggtgctggt gctggtgaag
      841 gagctgaacg agcagtttaa gaggaagatc aaggtggtgc tgcctgtgga cctggtgctg
      901 atcatcgccg cctccttcgc ctgctattgt accaatatgg agaacacata cggcctggag
      961 gtggtgggcc acatcccaca gggaatccca tccccaaggg caccccctat gaatatcctg
     1021 tctgccgtga tcaccgaggc cttcggcgtg gccctggtgg gatatgtggc atccctggcc
     1081 ctggcacagg gatctgccaa gaagtttaag tacagcatcg acgataatca ggagttcctg
     1141 gcccacggcc tgagcaacat cgtgtctagc ttctttttct gcatcccttc cgccgcagca
     1201 atgggaagaa ccgcaggact gtactctacc ggcgccaaga cacaggtggc ctgcctgatc
     1261 agctgtatct ttgtgctgat cgtgatctat gccatcggcc cactgctgta ctggctgccc
     1321 atgtgcgtgc tggcctccat catcgtggtg ggcctgaagg gcatgctgat ccagttcagg
     1381 gatctgaaga agtattggaa tgtggacaag atcgattggg gcatctgggt gtctacctac
     1441 gtgtttacaa tctgcttcgc cgccaacgtg ggcctgctgt ttggcgtggt gtgcaccatc
     1501 gcaatcgtga tcggccgctt cccaagggca atgacagtga gcatcaagaa catgaaggag
     1561 atggagttta aggtgaagac cgagatggac agcgagacac tgcagcaggt gaagatcatc
     1621 tccatcaaca atcccctggt gtttctgaat gccaagaagt tctataccga tctgatgaac
     1681 atgatccaga aggagaatgc ctgcaaccag cctctggacg atatcagcaa gtgtgagcag
     1741 aatacactgc tgaactccct gtctaacggc aattgcaacg aggaggccag ccagtcctgc
     1801 ccaaatgaga agtgttacct gatcctggac tgttccggct tcaccttttt cgattattcc
     1861 ggcgtgtcta tgctggtgga ggtgtacatg gactgcaagg gcagatccgt ggatgtgctg
     1921 ctggcccact gtaccgcctc tctgatcaag gccatgacat actatggcaa cctggactcc
     1981 gagaagccca tctttttcga gtctgtgagc gccgccatct ctcacatcca cagcaataag
     2041 aacctgagca agctgtccga tcactctgag gtgtaagacc cagctttctt gtacaaagtt
     2101 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2161 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2221 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2281 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2341 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2401 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2461 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2521 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2581 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2641 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2701 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2761 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2821 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2881 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2941 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3001 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3061 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3121 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3181 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3241 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4141 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4201 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4261 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4321 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4381 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4441 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4501 gccagtctta agctcgggcc c
//
