LOCUS       sequence-345405-        5624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0775:Dendra
FEATURES             Location/Qualifiers
     source          1..5624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     664..683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2186..2209
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2466..3266
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3391..3979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3880..3899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg
       61 cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg
      121 tctcggtcac acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga
      181 cgaggctatc gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc
      241 ccctcccaag gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac
      301 gcctggccgc gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg
      361 ccgccaggag cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt
      421 tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg
      481 atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc
      541 ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc
      601 gggagaactc cctatcagtg atagagtttg tcctccctat cagtgataga taggcttaag
      661 gagtaatacg actcactata gggagaagga ggccattatg gcagtagccc tgaacaagat
      721 caccgaccgc gtccatttcg cccgcaccga gctcgtcaac tggacgctgg tgaccggtga
      781 cgacggcgta ctgctcatcg acgcggggtt cccgggtcaa cgtgaggacg tgctcgattc
      841 ggtgcggcaa ctgggattcg gtcccgacga catcaaggcc gtgctgctca cccacgccca
      901 cgtcgaccac ttcggcactg ccatctggct cgcgaaaacc tatggcacac cggtgtattg
      961 ccacgcagac gaagtcggcc acgccaggcg cgagtacctg gagcaggcca ccccggcggg
     1021 cgtggcccgt caggcctggc agccgcgctg gctgaagtgg gcactggcga tctcccgtca
     1081 gggcgcattc gatcattccg gcattccttc gacgcaggcg ttcacccccg agatcgggct
     1141 gccgggaaat cccaccgcga ttccgactcc cggacacacc ggcgggcact gctcctatct
     1201 ggtcgacggt gtcctggtgg ccggcgacgc tctggtgacg ggccacccga cctcctcgcg
     1261 acgcggccct cagttgctgc atcaggtgtt caaccacgac cagcaggcgt gtctgcgcag
     1321 cctcggtgcg ctggggctgc tcgacaccga ggtgctgctg cccggccacg gcgacgtctg
     1381 gcgcggctcg atccgtgaga tggcggacca ggcggcatcc tcggcgccgt ccccggcccg
     1441 caagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
     1501 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
     1561 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
     1621 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
     1681 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
     1741 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     1801 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     1861 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     1921 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     1981 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     2041 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     2101 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     2161 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     2221 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     2281 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     2341 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     2401 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     2461 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     2521 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     2581 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     2641 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     2701 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     2761 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     2821 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     2881 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     2941 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     3001 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     3061 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     3121 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     3181 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     3241 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     3301 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     3361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3661 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3721 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3781 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     3841 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3901 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3961 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4021 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4081 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     4141 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     4201 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     4261 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     4321 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     4381 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     4441 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     4501 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     4561 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     4621 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     4681 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     4741 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     4801 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     4861 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     4921 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     4981 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     5041 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     5101 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     5161 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     5221 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     5281 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     5341 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     5401 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     5461 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     5521 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     5581 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaa
//
