LOCUS       sequence-345406-        5795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0876:Dendra
FEATURES             Location/Qualifiers
     source          1..5795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1694..1717
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1974..2774
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2899..3487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3388..3407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg agaaggaggc cattatgacc aaatggacca ccgccaggat
       61 tcccgatcaa acaggccgcg tcgcgatcgt cacgggcgcc aacaccggcc tgggcctcga
      121 aaccgcgaag gcgctcgccg ccaagggcgc ccatgtggtg ctcgcggtac gcaatctgga
      181 caagggcaag gccgcggtcg actggatcgc gcgcagcgcg cccaccgccg atctggagtt
      241 gcagcagctc gacctgggct cgctggcctc ggtgcgcgca gccgccgacg acctcaaggg
      301 caagttcgac cggatcgacc tgctcgtcaa caatgcaggc gtcatgtggc cgccgcgaca
      361 gaccaccgcg gacggtttcg agctgcagtt cggcaccaac catctgggcc acttcgcgct
      421 gaccggcctg ctgctcgatc gcatgctgac ggttccgggc tcacgcgtcg tgacggtcag
      481 cagccaaggc caccgcatcc tcgcggcgat ccacttcgac gatctgcagt gggaacgccg
      541 ctacaaccgg gtcgcggcgt acgggcagtc gaagttggcc aacctgctgt tcacctatga
      601 gctgcagcgc cgcctcaccg gtcaccagac cacggccctg gccgctcatc cgggcgcgtc
      661 gaacaccgaa ctcgcgcgcc acctgcctgg cgcgctggag cgtctcgtga ccccgctggc
      721 gcaggacgcc gccctcgggg cgctgccgac cctgcgcgcc gcgaccgatc ccggcgccct
      781 cggtgggcag tacttcggcc ccgacgggat cggcgagacg cggggctacc ccaaggtggt
      841 ggcgtccagc gcccagtcgc acgacgccga tctgcagcgg aggctgtggg cggtgtccga
      901 ggaactcacg ggcgtgacgt tccccgtcgg cgaggcgttg cagcaggcca agcttggtgc
      961 ggccgcgggc aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca
     1021 tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc
     1081 ttacgaaggg acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc
     1141 gtacgatatc ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga
     1201 ggatatccct gactacttca agcagagctt cccggaggga tactcctggg agcggacgat
     1261 gaccttcgaa gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg
     1321 tttcttccag aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca
     1381 gaagaagacc ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct
     1441 ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt
     1501 caagaccacg tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca
     1561 ccgcatcgaa atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc
     1621 tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa
     1681 cctgtatttc caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc
     1741 ctctaaacgg gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc
     1801 acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg
     1861 aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt
     1921 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat
     1981 cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc
     2041 ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg
     2101 tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc
     2161 cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg
     2221 ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa
     2281 atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga
     2341 tcatctctga tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc
     2401 atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc
     2461 atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc
     2521 aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg
     2581 ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg
     2641 cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc
     2701 gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc
     2761 gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg
     2821 cgctgattgc tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga
     2881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3001 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3301 cgccacgctt cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3601 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac
     3661 ccggggatcc tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg
     3721 ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga
     3781 gggttccgac cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac
     3841 cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt
     3901 ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag
     3961 aagagtcgca caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc
     4021 ctctgaccag cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg
     4081 tgtctgccga ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc
     4141 tctaagtgga aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga
     4201 gttgcaaccc ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg
     4261 ggatcgctga agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac
     4321 ggtgtgaggt actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc
     4381 gcgggcgaga agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag
     4441 aaggcagccg ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc
     4501 gacctcgcag acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg
     4561 gtgctaggtg aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc
     4621 gggatgggta ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg
     4681 atgaacacag cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag
     4741 gcagccgatg agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct
     4801 gagatcttcg agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc
     4861 ggagagctga tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc
     4921 cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc
     4981 gtccggtcga agcgtcctgt gacggttccg cctcacgtcg cggagatgat ccgagcgcac
     5041 atgaaggacc gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag
     5101 ggcaaccggc tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc
     5161 ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag
     5221 gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg
     5281 atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag
     5341 ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga
     5401 ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc
     5461 tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag
     5521 aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg
     5581 tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc
     5641 ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca
     5701 caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt
     5761 tgtcctccct atcagtgata gataggctta aggag
//
