LOCUS       sequence-345407-        5582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0916:Dendra
FEATURES             Location/Qualifiers
     source          1..5582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     663..682
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        663..681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2143..2166
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2423..3223
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3348..3936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3837..3856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc
       61 gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt
      121 ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac
      181 gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc
      241 cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg
      301 cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc
      361 cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt
      421 gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
      481 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
      541 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
      601 ggagaactcc ctatcagtga tagagtttgt cctccctatc agtgatagat aggcttaagg
      661 agtaatacga ctcactatag ggagaaggag gccattatgg cacagcagac tccaccggcg
      721 acggtgaaga ccgatggacg caaacggcgc tggcaccaac acaaggtgga gcgtcgtaat
      781 gaattggtgg acggcaccct cgaagccatc cgccgccggg gcagcaacgt cagcatggac
      841 gagatcgccg ctgagatcgg cgtgtccaag accgtgctgt atcgctactt cgtggacaag
      901 aacgatctca ccaccgcggt gatgatgcgg ttcgcgcaga ccacgctgat ccccaacatg
      961 gcggccgcgc tgtcgtcgaa cctcgacggg ttcgctctca cacgcgagat catccgcgtc
     1021 tacgtcgaga ccgtcgccgc cgagcccgaa ccgtaccggt tcgtgatggc caacaactcc
     1081 gcgagcaaga gcaaggccgt cgccgattcc gagcagatca tcgcgcgcat gctcgcggtg
     1141 atgctgcgcc gccgcatggc cgaggtcggc atggacaccg gcggtgtcga ggcctgggcg
     1201 ttccacaccg tcggcggtgt ccagctcgcc acgcactcgt ggatgtcgaa cccgcggatg
     1261 acctccgacg agctgatcga ctacctgacc atgctgtcgt ggtacgcgct gtgcggcatc
     1321 gtcgaggtcg gcggatcgct ggagaagttc aactcgcagc cgcatccatc gccgattccg
     1381 ccgcggcacc tgcttgacaa gcttggtgcg gccgcgggca acacgcctgg catcaatctg
     1441 atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg tcacgccttc
     1501 gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc caacctcacc
     1561 gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc ggtgcattac
     1621 gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa gcagagcttc
     1681 ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat ctgtacgatc
     1741 cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt caaggggacc
     1801 aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga gccgagcacg
     1861 gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc gctgctgctg
     1921 gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc
     1981 cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa cgattcggac
     2041 tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct cccgtcgcag
     2101 gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa ggacgacgac
     2161 gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtta
     2221 actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt gcagtaccag
     2281 cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc
     2341 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg
     2401 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga
     2461 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt
     2521 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact
     2581 gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc
     2641 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt
     2701 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt
     2761 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctta
     2821 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg
     2881 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta
     2941 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct
     3001 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag
     3061 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat
     3121 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga
     3181 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag
     3241 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gggggatcca
     3301 ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3481 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag aaaggcggac
     3781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4081 accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga ccaccaaggg
     4141 caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag ccgtgaacga
     4201 cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc ccggtgcaac
     4261 cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat gcccctcgtc
     4321 tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc agatcaggga
     4381 tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca gacccctgga
     4441 aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg
     4501 aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg ccggtcaaga
     4561 taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa
     4621 gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag
     4681 aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga
     4741 tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg
     4801 acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga
     4861 cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg
     4921 agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca
     4981 gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga
     5041 cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc
     5101 ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg
     5161 cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga
     5221 cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat
     5281 acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca
     5341 tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga
     5401 acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc
     5461 ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc
     5521 ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca
     5581 ag
//
