LOCUS       sequence-345408-        5621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0935:Dendra
FEATURES             Location/Qualifiers
     source          1..5621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     209..228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        209..227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1728..1751
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2008..2808
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2933..3521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3422..3441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca tccccacggt
       61 gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac gcacaggccc
      121 ggtgtgagaa gggtctctga cgagcgggag aactccctat cagtgataga gtttgtcctc
      181 cctatcagtg atagataggc ttaaggagta atacgactca ctatagggag aaggaggcca
      241 ttatggcgac cctgatcctg ctccgccacg gtgagagcca gtggaacgag aagaacctgt
      301 tcaccggttg ggtggacgtc gacctcactg acaagggccg ggccgaggcc gtccgcggcg
      361 gccaactgat ggccgagcag ggcgtcctgc ccgacgtgct ctacacgtca ctgctgcgcc
      421 gggccatcac caccgcgcac ctcgcgctcg acgccgctga ccggctgtgg atcccggtgc
      481 accgcgactg gcggctcaac gagcgccact acggcgcgct gcagggcctg gacaaggccg
      541 agaccaagga gaagtacggc gaagagcagt tcatggcgtg gcgccgcagc tacgacaccc
      601 cgccgccgcc catcgagaag ggcagcaagt acagccagga cgccgatccg cgctacgccg
      661 acatcgacgg cggcccgttg accgaatgcc tcaaagacgt cgtcgagcgg ttcgtgccgt
      721 actacgagag cgcgatcttg cccgatctga aggcgggcaa gaccgtgctg atcgccgccc
      781 acggcaactc gctgcgcgcg ctggtgaagt acctcgacgg catgtccgac gaggaggtcg
      841 tcggcctcaa catcccgacc ggcatcccgc tgcgctacga cctcgacgag aacctcaagc
      901 ccgtcaagat cggcggtgag tacctcgatc ccgaggcggc cgccgccggt gcggccgccg
      961 tcgccgccca gggcgcgaag aagaagcttg gtgcggccgc gggcaacacg cctggcatca
     1021 atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg
     1081 ccttcgtgat cgagggggag ggtaagggta agccttacga agggacccag accgccaacc
     1141 tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc
     1201 attacgggaa ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga
     1261 gcttcccgga gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta
     1321 cgatccgcag cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg
     1381 ggaccaactt tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga
     1441 gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc
     1501 tgctggaagg cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg
     1561 tcgtccagct gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt
     1621 cggactacaa caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt
     1681 cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg
     1741 acgacgacaa gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt
     1801 tgttaactag cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt
     1861 accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca
     1921 tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag
     1981 tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa
     2041 tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc
     2101 ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt
     2161 ggactgatcg aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg
     2221 tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt
     2281 ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt
     2341 gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca
     2401 ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc
     2461 ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt
     2521 ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat
     2581 tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag
     2641 aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc
     2701 gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg
     2761 ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag
     2821 cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg
     2881 atccactagt tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3061 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg
     3361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3661 gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc
     3721 aagggcacca tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg
     3781 aacgacaggg cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt
     3841 gcaaccttgt cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc
     3901 tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc
     3961 agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc
     4021 ctggaaagaa aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg
     4081 aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt
     4141 caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg
     4201 tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg
     4261 acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg
     4321 gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac
     4381 ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat
     4441 ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct
     4501 ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct
     4561 gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga
     4621 gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc
     4681 ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct
     4741 gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc
     4801 gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc
     4861 ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa
     4921 ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt
     4981 ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt
     5041 tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa
     5101 gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt
     5161 caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga
     5221 cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat
     5281 ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc
     5341 ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa
     5401 aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag
     5461 aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc
     5521 ccgccgccgc caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc
     5581 ccgctttgat cggggacgtc gaaatattgg atcgtcggca c
//
