LOCUS       sequence-345409-        5117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1017:Dendra
FEATURES             Location/Qualifiers
     source          1..5117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     355..374
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        355..373
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1370..1393
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1650..2450
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2575..3163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3064..3083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga
       61 gcattgccgt tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg
      121 acgtcgaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac
      181 gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg
      241 atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact
      301 ccctatcagt gatagagttt gtcctcccta tcagtgatag ataggcttaa ggagtaatac
      361 gactcactat agggagaagg aggccattat gaccgtcacc gtgtacacca agcccgcatg
      421 cgtgcagtgc aacgccacct acaaggcgct ggacaagctg ggcatcgcct acgagacggt
      481 cgacatcacc gtcgacagcg aggcccgtga ctacgtcatg gcgctcggct acctgcaggc
      541 accggtcgtg gtggccggca acgagcactg gtcgggcttc cgccccgacc gcatcaaggc
      601 actcagcgcc gcgacgatca gcgcgaagct tggtgcggcc gcgggcaaca cgcctggcat
      661 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
      721 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
      781 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
      841 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
      901 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
      961 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     1021 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     1081 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     1141 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     1201 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     1261 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     1321 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     1381 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     1441 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     1501 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     1561 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     1621 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     1681 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     1741 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     1801 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     1861 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     1921 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     1981 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     2041 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     2101 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     2161 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     2221 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     2281 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     2341 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     2401 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     2461 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     2521 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2701 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     3001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3301 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     3361 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     3421 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     3481 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     3541 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     3601 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     3661 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     3721 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     3781 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     3841 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     3901 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     3961 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     4021 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     4081 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     4141 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     4201 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     4261 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     4321 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     4381 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     4441 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     4501 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     4561 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     4621 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     4681 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     4741 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     4801 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     4861 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     4921 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     4981 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     5041 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     5101 agaaccacgc ctggccg
//
