LOCUS       sequence-34541-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Rui X, Tsao J, Scheys JO, Hammer GD, Schimmer BP
  TITLE     Contributions of specificity protein-1 and steroidogenic factor 1 to
            Adcy4 expression in Y1 mouse adrenal cells.
  JOURNAL   Endocrinology. 2008 Jul . 149(7):3668-78.
  PUBMED    18388192
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2008 Jul . 149(7):3668-78."
COMMENT     Sequence Label: SF-1 shRNA #1
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..156)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     rep_origin      245..673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(332..351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     542..563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(681..701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tggttagtga accgtggatc ctaatgcttg ttgttctgga ctttgatatc cgagtccaga
       61 acaacaagca ttacgcttaa gaataaagga tcttttattt tcattggatc tgtgtgttgg
      121 ttttttgtgt gcggccgccc tcgactgtgc cttctagaag acaatagcag gcatgctggg
      181 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
      241 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      301 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      361 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      421 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      481 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      541 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      601 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      661 tttaacgcga attaattcgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
      781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      841 catagtccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgccccattc
      901 tccgccccat ggctgact
//
