LOCUS       sequence-345411-        5485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0970:Dendra
FEATURES             Location/Qualifiers
     source          1..5485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     973..992
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        973..991
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2356..2379
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2636..3436
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3561..4149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4050..4069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcggcatac atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg
       61 caaggacatc gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg
      121 cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac
      181 ggttccgcct cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa
      241 caagggcccc gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc
      301 gttcaccaag tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca
      361 cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct
      421 gatggcccgt ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga
      481 ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc
      541 aaaaagcccc cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg
      601 aagaaccacg cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca
      661 gccccgccgc cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg
      721 cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga
      781 ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc
      841 tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct
      901 ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc agtgatagat
      961 aggcttaagg agtaatacga ctcactatag ggagaaggag gccattatgc gcaccaccgt
     1021 ccacgtgatg cgccacggcg aggtgcacaa ccccgccaag gtgctctacg ggcggctgcc
     1081 cggctatcac ctgtccgagc gtggccgtgc gcaggcgcgg tccgctgccg actggctggc
     1141 cggccgcgac atcgtctacc tcgtcgcgtc accgctggag cgcgcacagg agaccgcggc
     1201 gcccatcgcc gagagtcacg gcctgtcgat cgacaccgac gacgacctca tcgagtcgtg
     1261 gaacaccttc gagggccaga aggtcgcgcc gggtgacggc gcgctgcgcg atccgcgcaa
     1321 ctggccgcgc ctgcgcgatc cccgcaagcc gtcgtggggt gagccgtacc acgaggtcgc
     1381 ggcacgcatg atggccgcgc tgcaccgcgc ccggctcaag gccgcggggc acgaggcggt
     1441 gtgcgtgagc caccagttgc cggtcgagac gctgcggcgc gcgatgacgg gccgcaagct
     1501 cgcgcatctg ccgttgccga acagcaggct gtgcaatctg gcgtccatca cgtcgttcac
     1561 gttcgacgac gaccggctga tccgctgggg atattcggag ccctggggaa tcagcttggt
     1621 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
     1681 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1741 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1801 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1861 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     1921 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     1981 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     2041 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     2101 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     2161 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     2221 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     2281 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     2341 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     2401 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     2461 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     2521 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     2581 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     2641 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     2701 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2761 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2821 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2881 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2941 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     3001 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     3061 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     3121 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     3181 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     3241 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     3301 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     3361 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     3421 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     3481 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     3541 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3601 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3661 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3721 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3781 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3841 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3901 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3961 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4021 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4081 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4141 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4201 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4261 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     4321 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     4381 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     4441 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     4501 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     4561 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     4621 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     4681 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4741 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4801 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4861 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     4921 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     4981 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     5041 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     5101 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     5161 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     5221 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     5281 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     5341 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     5401 aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg
     5461 ctgagatctt cgagcactac cggat
//
