LOCUS       sequence-345414-        5762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1203:Dendra
FEATURES             Location/Qualifiers
     source          1..5762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     746..765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        746..764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2406..2429
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2686..3486
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3611..4199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4100..4119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
       61 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
      121 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
      181 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
      241 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
      301 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
      361 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
      421 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
      481 ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt
      541 cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct
      601 gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt
      661 gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt tgtcctccct
      721 atcagtgata gataggctta aggagtaata cgactcacta tagggagaag gaggccatta
      781 tggctgacgg cgtcggcact gcccagatgc ggccgtattt cgaggaagtc caggcgcact
      841 acgatctgtc cgacgacttc ttcgggctgt tccaggatcc gtcccgcacc tacagctgtg
      901 cctactacga gcgcgacgac atgacactcg aacaggcgca gatggccaag atcgacctcg
      961 cactggacca gctcgccctg caaccgggaa tgacgctgct cgacatcggt tgcggctggg
     1021 gcgcgttgat gaaacgcgcg atcgagaagt acgacgtcga cgtcatcggc ctcaccctga
     1081 gcaagaacca gtacgcgttc tgccaacagc ttctggatca gatcgacacg cggcggtcac
     1141 acttcgtgtg cctgcagggc tgggagcagt tcgacgcacc ggtcgaccga atcgtgagca
     1201 tcgaggcgtt cgaggcgttc gggaagtcga ggtacaaggc gtttttcgac accacctacc
     1261 gggtgatgcc cgacgacggg cgcatggtga tcgagacgat cttcacccat cccatcaact
     1321 actggcgtga gcacggcatc gcggtgacgc tcagcgacat gaagttcacg cggttcatct
     1381 acaaggagat cttcccgggc gggcagctgc cgtccgacga cgatatgcgt gacttctccg
     1441 gtgccgcagg gtttcacacc gccgacgtga ccgtgatgaa cccacactat gttcgcacgc
     1501 tcgacacgtg ggccgagaac ctgtccgcgc aacgcgccgc ggcgatcgcc acgacgtcgg
     1561 aagaggtcta cgaccggtac atgcactatc tgaccggctg cgccgacttc ttccgccgcg
     1621 ggatctccga ggtcgcacag ttcgcctaca cgaagagcgg taagcttggt gcggccgcgg
     1681 gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg catatggagg
     1741 gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag ccttacgaag
     1801 ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc tcgtacgata
     1861 tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc gaggatatcc
     1921 ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg atgaccttcg
     1981 aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac tgtttcttcc
     2041 agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg cagaagaaga
     2101 ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc ctggtcggca
     2161 acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac ttcaagacca
     2221 cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac caccgcatcg
     2281 aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac gctgtggcac
     2341 gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag aacctgtatt
     2401 tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg gcctctaaac
     2461 gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac
     2521 tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc
     2581 ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc
     2641 accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg
     2701 gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac
     2761 cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc
     2821 ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg tccgggaggg
     2881 acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg
     2941 cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag
     3001 cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct
     3061 gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc
     3121 gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc
     3181 gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc
     3241 aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg
     3301 gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct
     3361 cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg
     3421 ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg
     3481 attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt
     3541 gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta gaaaagatca
     3601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3721 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4021 ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4261 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4321 ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt acccggggat
     4381 cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc
     4441 agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg
     4501 accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca
     4561 atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc
     4621 atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg
     4681 cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc
     4741 agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc
     4801 gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat cctctaagtg
     4861 gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag gagttgcaac
     4921 ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat ggggatcgct
     4981 gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg acggtgtgag
     5041 gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc tcgcgggcga
     5101 gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga agaaggcagc
     5161 cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc
     5221 agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc cggtgctagg
     5281 tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg
     5341 taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac
     5401 agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga
     5461 tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt
     5521 cgagcactac cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct
     5581 gatcgagctt cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg
     5641 ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc
     5701 gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga
     5761 cc
//
