LOCUS       sequence-345415-        5585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1347:Dendra
FEATURES             Location/Qualifiers
     source          1..5585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     336..355
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        336..354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1819..1842
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2099..2899
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3513..3532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag
       61 aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg
      121 tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc
      181 ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca
      241 caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt
      301 tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta tagggagaag
      361 gaggccatta tgagcaagaa cagcaaggca taccgcgagg ccgcggagaa ggtcgaccgg
      421 accaagctct acacgccgct ggaagccgcg aaactggcca aggagacctc ctccaagaag
      481 caggacgcca ccgtcgaggt tgccatccgc ctcggcgtcg acccccgcaa ggccgaccag
      541 atggtccgcg gcaccgtgaa cctgccccac ggcaccggta agaccgcgcg cgtcgcggtg
      601 ttcgccgtcg gtgagaaggc cgagcaggcc caggccgcag gcgccgacat cgtcggcagc
      661 gacgacctga tcgagaagat ccagggcggc ttcctggact tcgacgccgc gatcgccaca
      721 ccggaccaga tggccaaggt cggccgtatc gcccgcgtgc tcggcccgcg tggcctgatg
      781 ccgaacccca agaccggcac cgtcaccccc gatgtggcca aggctgtgca ggacatcaag
      841 ggcggcaaga tcaacttccg cgtcgacaag caggccaacc tgcacttcat catcggcaag
      901 gcgtcgttcg acgagaccaa gctggccgag aactacggcg cggcgctcga cgaggttctg
      961 cgcgccaagc cgtcgtcgtc caagggccgt tacctcaaga aggtgaccgt ctcgacgacc
     1021 accggcccgg gcatccccgt cgacccctcg gtcacccgga acttcaccga ggccaagctt
     1081 ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat gcgtgtcaag
     1141 gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga gggtaagggt
     1201 aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc ccctctccca
     1261 ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt caccaagtac
     1321 cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc ctgggagcgg
     1381 acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc gctggaaggg
     1441 gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa cggcccggtc
     1501 atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt tcgggacggc
     1561 ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca ctacctatgc
     1621 gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc gcacttcgtg
     1681 gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa gttgtacgag
     1741 cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg cggcggcggg
     1801 gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa ataacccctt
     1861 ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc gaattcctgc
     1921 agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag aatgatgtca
     1981 cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa aaggggatga
     2041 taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg atcgactgat
     2101 gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg
     2161 tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca gctccttccg
     2221 tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc gtgctacgct
     2281 gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg agccgttcga
     2341 tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat tctggcgcct
     2401 gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc cacaggcaga
     2461 gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc cggtcgcccg
     2521 tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg ccaacacgac
     2581 gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac actgcaccat
     2641 tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact gctgtgagcg
     2701 ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag gtccagtcgg
     2761 tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc tgggtcaact
     2821 gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc
     2881 cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt ggaggggcaa
     2941 ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3421 gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgcggccgc
     3781 ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt gggccacccc
     3841 gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca gcccgccgac
     3901 ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta ttctcttcac
     3961 tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg gggggctcta
     4021 ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc gtatgcccag
     4081 gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca gagggcgaaa
     4141 acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga
     4201 atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc ctctcggctg
     4261 catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct gagagttgca
     4321 caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg gcacgtcgcg
     4381 gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac gtcaacccgc
     4441 aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac gccgaagcct
     4501 ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca caggaccggg
     4561 cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag tggctcgtgg
     4621 agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag cgccgcatct
     4681 acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg cgtgcgtggt
     4741 gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac gtcctccggg
     4801 cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc cggatcgagc
     4861 agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg gacatcgtcg
     4921 ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg acgagcctcc
     4981 ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc atgacgatga
     5041 agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt ggcaacgcca
     5101 agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag atgatccgag
     5161 cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg gtgaccacga
     5221 cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca
     5281 agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct acgttcgccg
     5341 ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg actcctagga
     5401 tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt
     5461 ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac actgagtcct
     5521 aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc
     5581 gccgc
//
