LOCUS       sequence-345416-        5714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0825:Dendra
FEATURES             Location/Qualifiers
     source          1..5714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     650..669
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        650..668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2262..2285
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2542..3342
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3467..4055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3956..3975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct gtcggcgcta
       61 cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc ggtcacacga
      121 ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag gctatcgctg
      181 aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct cccaaggaca
      241 ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc
      301 gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc caggagcatt
      361 gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat cggggacgtc
      421 gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat
      481 gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg cgttgatctg
      541 tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga gaactcccta
      601 tcagtgatag agtttgtcct ccctatcagt gatagatagg cttaaggagt aatacgactc
      661 actataggga gaaggaggcc attatgaaac tgctaccgct gaccccgccc ggtcagacgc
      721 cgacccgggt gatgaccatc gcgggcaccg attccggtgg cggcgcgggc atccaggccg
      781 atctgcgcac gttcgccatg ctgggcgtgc acggctgtgt cgcggtggca gccgtcacgg
      841 tgcagaattc ggtgggggtc aagggatttc acgaggttcc ggtcgacatc atcgcagggc
      901 agatccaggt ggtcgccgag gacatcggca tccaggccgc caagaccggc atgctggcgt
      961 cagcagagat catcggtgcg gtcgccgaga cctggcgcgg gctgggcacc gaagccccgc
     1021 tggtggtcga tccggtgtgc gcgtcgatgc acggcgatcc gctgctgcat cccagtgccc
     1081 tcgattcggt gcgcaacgaa ctgtttccga tcgcctcact ggtgacaccg aacctcgacg
     1141 aggtccggct catcaccggc atcgaggtgg tggacgaggc cacgcagcgc gacgccgcca
     1201 aggcgctgca cgctctgggt cccaagtggg cactggtcaa gggcgggcac ctgcgcacgt
     1261 cggcgcagag ccccgatctg ctgtacaacg gcaccgactt ctacgagttc ggcgccgagc
     1321 ggatcgacac cggacacgac cacggcgccg gggacaccct cgccgcggcc gcggcctgcg
     1381 cgctggcaca cggcatggat atgcccgacg cggtagcgtt cgccaaaggg tgggtgaccg
     1441 aatgtctgcg tgcggcatat ccgttgggcc acggccacgg cccggtgtcg gccctgttca
     1501 ggttgcacca tggctcgaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga
     1561 tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg
     1621 tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg
     1681 tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg
     1741 ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc
     1801 cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc
     1861 gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca
     1921 actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg
     1981 agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg
     2041 aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc
     2101 agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact
     2161 acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg
     2221 tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg
     2281 acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa
     2341 ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     2401 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     2461 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     2521 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     2581 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     2641 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     2701 atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     2761 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     2821 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     2881 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac
     2941 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     3001 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     3061 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     3121 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     3181 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     3241 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     3301 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     3361 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac
     3421 tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3601 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca
     3901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4081 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4141 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4201 ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc
     4261 accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac
     4321 agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc
     4381 ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct
     4441 gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat
     4501 gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa
     4561 agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga
     4621 acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat
     4681 aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag
     4741 cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga
     4801 ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat
     4861 ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga
     4921 caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac
     4981 ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga
     5041 gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag
     5101 cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac
     5161 gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg
     5221 gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc
     5281 agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac
     5341 gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata
     5401 catcctggcg tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat
     5461 cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa
     5521 caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc
     5581 tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc
     5641 cgaggcattc ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa
     5701 gtcgctgaag cgtg
//
