LOCUS       sequence-345417-        6452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0826:Dendra
FEATURES             Location/Qualifiers
     source          1..6452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2104..2127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2384..3184
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3309..3897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3798..3817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6206..6225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6206..6224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcggcacgc agctgcaacg cgcccgtgcc ggtgagatca ccgccgagat ggcgttcatc
       61 gccgagcgcg aaggcgtccc cgccgaactg gtgcgttccg aggtggcacg tgggcgggcg
      121 gtgatcccgg ccaaccacaa tcaccccgag agcgaaccga tgatcatcgg caaggccttc
      181 ggcgtgaaag tcaatgcgaa catcggcaat tcggcggtca catcgtcgat cgccgaagaa
      241 gtcgacaaga tggtgtgggc gacccgctgg ggtgccgaca ccatcatgga cctgtcgacc
      301 ggtcgcgaca tccacctgac gcgggaatgg atcctgcgca actcgccggt gccggtgggc
      361 accgtgccca tgtaccaggc actggagaag gtcaacggcg accccaccaa gctcagctgg
      421 gatgtctacc gcgacaccgt gatcgagcag tgcgaacagg gcgtggacta catgaccgtg
      481 cacgccggtg tgctgctgcg gcacatcccg ctgacggtcg accgggtcac gggcatcgtg
      541 tcacgcggcg ggtcgatcat ggcggcctgg tgcctggcac atcacacaga gtcgttcctg
      601 tacaccaact tcgaggaact gtgtgagatc ttcgcacgct acgacgtgac gttctcgctg
      661 ggcgacggcc tgcgcccggg gtccatcgcc gacgccaacg acgcggccca attcgccgaa
      721 ctgcgcaccc tcggcgagct caccacgatc gcgaaaaacc atggcgtgca ggtgatgatc
      781 gaaggccctg gccacgtccc catgcacaag atcgtcgaga acgtgcgcct cgaagaggaa
      841 ctgtgtgacg atgcgccgtt ctacacactg ggaccgctcg cgaccgacat cgccccggcg
      901 tacgaccaca tcacctcggc gatcggcgcg gcgatcatcg cccaggcagg caccgcgatg
      961 ctgtgctacg tcacacccaa ggagcatctg ggcctgccca accgccagga cgtcaaggac
     1021 ggcgtgatcg cctacaagat cgcggcgcac tctgccgacc tggccaaggg ccatccccgc
     1081 gcgcaacagc gcgatgacgc actgtccaag gcgcgcttcg agttccggtg gcacgaccag
     1141 ttcgcgctgt cgctggatcc cgacaccgcg cgcgagttcc acgacgagac actgcccgcc
     1201 gaaccggcga agaccgcgca cttctgctcg atgtgcgggc ccaagttctg ctccatgcgc
     1261 atcacggccg acctcaagga ttcgatccgc gaggcgatgg accagaaatc cgccgagttc
     1321 gccgaacagg ggaaccgtgt gtatctaccg ttgacatcga agcttggtgc ggccgcgggc
     1381 aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca tatggagggc
     1441 aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc ttacgaaggg
     1501 acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc gtacgatatc
     1561 ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga ggatatccct
     1621 gactacttca agcagagctt cccggaggga tactcctggg agcggacgat gaccttcgaa
     1681 gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg tttcttccag
     1741 aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca gaagaagacc
     1801 ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct ggtcggcaac
     1861 atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt caagaccacg
     1921 tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa
     1981 atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc
     2041 tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa cctgtatttc
     2101 caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc ctctaaacgg
     2161 gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc acggtaactg
     2221 atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg
     2281 cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac
     2341 cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga
     2401 gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc ttctcaacct
     2461 tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct
     2521 cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc cgggagggac
     2581 gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc
     2641 cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg
     2701 cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga
     2761 tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc atgaactcga
     2821 tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga
     2881 gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa
     2941 gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga
     3001 caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg
     3061 gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt
     3121 gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat
     3181 tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg cgctgattgc
     3241 tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga aaagatcaaa
     3301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3421 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3661 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3721 cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3781 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3841 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3901 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3961 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4021 accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc
     4081 tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg ccgcagccag
     4141 ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga gggttccgac
     4201 cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac cagctccaat
     4261 ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat
     4321 cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag aagagtcgca
     4381 caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag
     4441 cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg tgtctgccga
     4501 ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc tctaagtgga
     4561 aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga gttgcaaccc
     4621 ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg ggatcgctga
     4681 agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac ggtgtgaggt
     4741 actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc gcgggcgaga
     4801 agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag aaggcagccg
     4861 ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc gacctcgcag
     4921 acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg gtgctaggtg
     4981 aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc gggatgggta
     5041 ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg atgaacacag
     5101 cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag gcagccgatg
     5161 agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct gagatcttcg
     5221 agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc ggagagctga
     5281 tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc cgggtgcgcc
     5341 gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc gtccggtcga
     5401 agcgtcctgt gacggttccg cctcacgtcg cggagatgat ccgagcgcac atgaaggacc
     5461 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
     5521 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
     5581 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
     5641 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
     5701 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
     5761 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
     5821 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
     5881 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
     5941 ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt
     6001 cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct
     6061 gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt
     6121 gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt tgtcctccct
     6181 atcagtgata gataggctta aggagtaata cgactcacta tagggagaag gaggccatta
     6241 tgacgaccgg cccgatcacg gggagtacca aggcctatcg cgaggtggag catccggggg
     6301 tggacgtccc gctgcgggtg ccgttccgcc gggtccacct gagcgacggc gaccacctcg
     6361 acctgtacga cacgtccggt ccgtacaccg acaccgacgc cgtcatcgac ctcgacgcgg
     6421 gcctgacacc gcgacccggg gtggtgcgcg ac
//
