LOCUS       sequence-34542-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Rui X, Tsao J, Scheys JO, Hammer GD, Schimmer BP
  TITLE     Contributions of specificity protein-1 and steroidogenic factor 1 to
            Adcy4 expression in Y1 mouse adrenal cells.
  JOURNAL   Endocrinology. 2008 Jul . 149(7):3668-78.
  PUBMED    18388192
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2008 Jul . 149(7):3668-78."
COMMENT     Sequence Label: SF-1 shRNA #3
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(144..161)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     rep_origin      250..630
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(337..356)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     547..568
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt ggatcctcaa tgagaaggtt gttgcggttg atatccgccg
       61 caacaacctt ctcattgacg cttaagaata aaggatcttt tattttcatt ggatctgtgt
      121 gttggttttt tgtgtgcggc cgccctcgac tgtgccttct agaagacaat agcaggcatg
      181 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      241 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      301 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      361 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      421 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      481 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      541 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      601 ttgatttata agggattttg ccgatttcgg cctattggtt aaa
//
