LOCUS       sequence-345421-        5411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0948:Dendra
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1283..1302
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1283..1301
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2592..2615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2872..3672
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3797..4385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4286..4305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag
       61 tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga
      121 agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg aacacagcgg
      181 tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca gccgatgagc
      241 gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag atcttcgagc
      301 actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga gagctgatcg
      361 agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg
      421 gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc
      481 gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg aaggaccgta
      541 cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc aaccggctgt
      601 cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt cggccggaac
      661 tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga
      721 ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg aagtaccaga
      781 tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg gccaagacct
      841 cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg gggtttcttg
      901 tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct gtgcggagac
      961 ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagaaa tctagacggt
     1021 gaccacaacg cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac
     1081 ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg
     1141 cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg
     1201 agaagggtct ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc
     1261 agtgatagat aggcttaagg agtaatacga ctcactatag ggagaaggag gccattatga
     1321 gcatcgcggc aaacatcatc gggactcact accgctaccc cgactatttc gaggtcggac
     1381 gggagaaggt ccgcgagttc tcggcggccg tcaaggacga ccaccccgcg catttcgacg
     1441 aggcggcggc caaggagtgc ggccacgaca acctcatcgc gccgctgacc ttcctggcgg
     1501 tcgcgggccg tcgagtgcag ctcgacctgt tcgacaagtt cgacgtgccg atcaacctcg
     1561 agcgtgtgct gcaccgggac cagaagctga tcttccaccg tccgatcgtg gtcggtgaca
     1621 agctgtggtt cgactcgtac ctggactcgg tgatcgagtc gcacggcacg gtgatctgcg
     1681 agatccgtgc cgaggtgacc gacgacgacg gcaagcccgt ggccaccagc atcgtcacga
     1741 tgctcggcga ggccgagatc gacgaggccg acgagatcag ttcccagatc gccgcggcgc
     1801 gcgatgccgc catcgccaag atggttgccg ggcaaaagtc cagttccaag cttggtgcgg
     1861 ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata
     1921 tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt
     1981 acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt
     2041 acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg
     2101 atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga
     2161 ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt
     2221 tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga
     2281 agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg
     2341 tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca
     2401 agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc
     2461 gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg
     2521 tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc
     2581 tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct
     2641 ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac
     2701 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
     2761 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
     2821 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
     2881 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
     2941 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     3001 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg
     3061 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     3121 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     3181 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     3241 atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat
     3301 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     3361 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     3421 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     3481 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     3541 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     3601 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     3661 cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg
     3721 ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4501 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc
     4561 ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc
     4621 gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg
     4681 gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca
     4741 gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt
     4801 gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa
     4861 gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct
     4921 ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg
     4981 tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc
     5041 taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt
     5101 tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg
     5161 atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg
     5221 tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc
     5281 gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa
     5341 ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga
     5401 cctcgcagac g
//
