LOCUS       sequence-345424-        6521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1109:Dendra
FEATURES             Location/Qualifiers
     source          1..6521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     709..728
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        709..727
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3128..3151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3408..4208
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4333..4921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4822..4841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg
       61 ctacgccaag atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac
      121 gttcgccgct caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac
      181 tcctaggatg gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga
      241 ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac
      301 tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg
      361 ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg
      421 ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg
      481 aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg
      541 atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt
      601 gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat
      661 cagtgataga gtttgtcctc cctatcagtg atagataggc ttaaggagta atacgactca
      721 ctatagggag aaggaggcca ttatggtgaa cccatcgacg acacaggccc gcatagtcgt
      781 cgacgaactc attcgcggcg gtgtccgcga cgtggtcctg tgccccggct cacgtaacgc
      841 accgctggcg tttgcgttgt ccgacgccga ccgggccggc cggatccggc tgcacgtgcg
      901 catcgacgag cgcaccgccg gctacctggc gatcggcctc gcggtggccg aacgcgcccc
      961 ggtgtgcatc gcgatgacct cggggaccgc ggtggccaac ctgggtccgg cggtcgtgga
     1021 ggccaactac gcgcgggttc cgctgatcgt gctgagcgcc aaccgtccct acgagatgct
     1081 cggcacgggc gccaaccaga ccttcgagca gctcggctac ttcggcaccc aggtccgcgc
     1141 ggccatcagc ctgggcctgg cacccgacct gaccggcccg aacgcggccg atctcgacac
     1201 cctcaacgcc cagtggcgct cggcgacgtg ccgtgtgctg gtcgcggcga ccggcgcgcg
     1261 cagcgccaac gcgggcccgg tgcagttcga catcccgctg cgcgaaccgc tcgtacccga
     1321 tccggccgag cccgcgggcg gcaccatccc gcccggacgg cccggcggcc ggtcgtggac
     1381 gcacacgccg ccggtgacgt tcgaccagcc cctggacatc gacctcacgc cggacaccgt
     1441 ggtgatcgcc gggcacggcg ccggtgagca cccgaacctg gccgagctgc cgacggtcgc
     1501 cgagcccacc gccccgccgc cggccaaccc gctgcatccg ctcgcgctgc ggctcgcgca
     1561 ccccaagcag gtgatcatgc tgggccgccc gacgctgcac cggccggtgt cgacgctgct
     1621 ggcggatccg tcggtgccgg tctacgcgct cacgacgggg ccgcgctggc ccgatgtctc
     1681 cggcaattcg caggccaccg gcacccgcgc ggtcaccagc ggcgccccga gcgcagcctg
     1741 gctgcgcaag tgcgccgaac tcaacgagca cgcggtcacc gcggtacgca agcaactggc
     1801 cgcacacccg atgaccacgg gcctgcacgt cgccgcggcc gtggccgacg cgctgcgtcc
     1861 cggtgatcag ctggtgctgg gcgcgtcgaa cccggtgcgc gacgcggcgc tggtgggtct
     1921 gcggcccgac ggcatcaagg tgcggtccaa ccgcggcgtc gccggcatcg acgggaccat
     1981 ctcgacggcc atcggtgccg cgctcgccca cgacggtcgg accgtggcgc tcataggcga
     2041 cctgacgttc gtgcacgaca gctcgggtct gctgatcggg ccgaccgagc cgacaccgcg
     2101 cagcctgacc atcgtggtgt ccaacgacaa cggcggtggc atcttcgagt tgctcgagca
     2161 gggtgatcca cggttctccg acgtgtcgtc gcggatcttc ggaacgccgc acgacgtcaa
     2221 catcggcgcg ctgtgccacg cctaccacgt cgactatcac cagatcgagg ccgacgcgct
     2281 cggcgcggcg ctcgaagacc cgttccaggg catgcgggtt ttggaggtga aggccgaccg
     2341 gtcatcgctg cgctcgctgc acgcgtcgat caaggcggcc ctgaagcttg gtgcggccgc
     2401 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     2461 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     2521 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     2581 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     2641 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     2701 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     2761 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     2821 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     2881 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     2941 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     3001 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     3061 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     3121 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     3181 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     3241 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     3301 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     3361 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     3421 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     3481 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     3541 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     3601 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     3661 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     3721 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     3781 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     3841 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     3901 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     3961 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     4021 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     4081 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     4141 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     4201 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     4261 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4741 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5041 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     5101 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     5161 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     5221 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     5281 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     5341 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     5401 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     5461 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     5521 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     5581 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     5641 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     5701 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     5761 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     5821 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     5881 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     5941 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     6001 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     6061 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     6121 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     6181 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     6241 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     6301 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     6361 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     6421 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     6481 gaccgtacga agatgaacaa gggccccgag gcattcctgg t
//
