LOCUS       sequence-345425-        5801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1205:Dendra
FEATURES             Location/Qualifiers
     source          1..5801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     664..683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2363..2386
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2643..3443
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3568..4156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4057..4076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg
       61 cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg
      121 tctcggtcac acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga
      181 cgaggctatc gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc
      241 ccctcccaag gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac
      301 gcctggccgc gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg
      361 ccgccaggag cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt
      421 tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg
      481 atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc
      541 ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc
      601 gggagaactc cctatcagtg atagagtttg tcctccctat cagtgataga taggcttaag
      661 gagtaatacg actcactata gggagaagga ggccattatg aaagaaacgc cacagaaatc
      721 gaacatcgcg gtcaaggtcg gagctccgcc cggcgagcgt cacggatcaa cagcagacga
      781 agtccgttcg cactacgacc tgtcgaacga gttcttccgg ctgtggcagg accccacgca
      841 gacctacagt tgcgcctact tcgaacgcga cgacatgacg ctcgaagagg cccagatggc
      901 gaaagtcgat ctggcacttg gcaagctggg cctgcaacca ggtatgactt tgctcgacat
      961 cggctgcggg tggggctcga ccatcatgcg cgcggtcgag aaatacgacg tcaacgtcat
     1021 cgggctgacg ctgtcgcaca atcagcgcgc acacatcgag cagcgcttcg ccgaatccga
     1081 cagcccacgc cgcaaagagg tccggctgca accatgggag gacttcgacg agccggtcga
     1141 ccgcatcgtg tcgatcgggg cgttcgaaca cttcggattc gagaagtacg cagactattt
     1201 caagaagacc ttcgagctga tgcccgacga cggcgtgatg ctgctgcaca ccatcgtctc
     1261 gaccagcaag gaagaggtcg aggagatggg gctgccgacg acgatgtcgt tgatgcggtt
     1321 cttccggttc atcgtcaccg agatctaccc gggtggccgc atcccgctga tcgcgatggt
     1381 cgaagaccgt gccaccgagg ccggtttcac cgtcacgcgc aagcagcgcc tgcggccgca
     1441 ctacgtgcgc accctcgaca catgggcggc caacctggag gccaagaagg accaggccat
     1501 cgagatcacg tccgaagagg tctacgagcg ctacctcaag tacctcaagg ggtgcgcgga
     1561 cctgttccgc gacggctaca ccgacgtctg ccagttcacc tgtgagaaga gcgtggccaa
     1621 gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt
     1681 caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa
     1741 gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct
     1801 cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa
     1861 gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga
     1921 gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga
     1981 aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc
     2041 ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga
     2101 cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct
     2161 atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt
     2221 cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta
     2281 cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg
     2341 cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc
     2401 ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc
     2461 ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat
     2521 gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg
     2581 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     2641 tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca
     2701 tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct
     2761 tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta
     2821 cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt
     2881 tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc
     2941 gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg
     3001 cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg
     3061 cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca
     3121 cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca
     3181 ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg
     3241 agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag
     3301 tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc
     3361 aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg
     3421 atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg
     3481 gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga
     3541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3961 atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag
     4021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4201 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4261 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     4321 ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca
     4381 ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc
     4441 cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct
     4501 tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc
     4561 tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc
     4621 ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc
     4681 gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     4741 aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg
     4801 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     4861 tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt
     4921 cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac
     4981 ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa
     5041 gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac
     5101 cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc
     5161 gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc
     5221 atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg
     5281 tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc
     5341 cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc
     5401 gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc
     5461 gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc
     5521 ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg
     5581 atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac
     5641 gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc
     5701 cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc ccgaggcatt cctggtgacc
     5761 acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca a
//
