LOCUS       sequence-345427-        4395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A12_STOP
FEATURES             Location/Qualifiers
     source          1..4395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1952..2051)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2068..2087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2069..2087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2210..2229)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2221..3030
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2288..2307)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3177..3765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3666..3685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3919..3936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4095..4122)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4214..4300)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4349..4371
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4363..4380
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4364..4380
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cggcaaggtg
      121 gcagtgcagg agtgtggacc acctgccgtg tcctgggtgc cagaggaggg agagaagctg
      181 gaccaggagg acgaggatca ggtgaaggat cgcggccagt ggaccaacaa gatggagttt
      241 gtgctgtctg tggccggcga gatcatcggc ctgggcaacg tgtggcggtt cccctacctg
      301 tgctataaga atggcggcgg cgccttcttt atcccctact ttatcttctt tttcgtgtgc
      361 ggcatccccg tgttcttcct ggaggtggcc ctgggccagt atacctccca gggctctgtg
      421 acagcctgga gaaagatctg cccactgttc cagggaatcg gactggcctc cgtggtcatc
      481 gagtcttacc tgaacgtgta ctatatcatc atcctggcct gggccctgtt ttatctgttc
      541 agctccttta ccagcgagct gccttggacc acatgtaaca atttctggaa caccgagcac
      601 tgcacagact ttctgaatca cagcggcgcc ggcaccgtga cacctttcga gaacttcacc
      661 tccccagtga tggagttctg ggagcggaga gtgctgggca tcacatccgg catccacgat
      721 ctgggctctc tgagatggga gctggccctg tgcctgctgc tggcctgggt catctgctac
      781 ttttgtatct ggaagggcgt gaagtccacc ggcaaggtgg tgtacttcac cgccacattt
      841 ccatatctga tgctggtcat cctgctgatc agaggagtga cactgccagg agcatatcag
      901 ggaatcatct actatctgaa gccagatctg ttcaggctga aggaccccca agtgtggatg
      961 gatgccggca cccagatctt tttctctttt gccatctgcc agggctgtct gacagccctg
     1021 ggcagctaca acaagtatca caacaattgc tacaaggact gtatcgccct gtgcttcctg
     1081 aatagcgcca cctcctttgt ggccggcttc gtggtgtttt ctatcctggg cttcatgagc
     1141 caggagcagg gcgtgcctat cagcgaggtg gcagagtccg gaccaggact ggccttcatc
     1201 gcctttccca aggccgtgac catgatgcct ctgtctcagc tgtggagctg tctgtttttc
     1261 atcatgctga tcttcctggg cctggacagc cagttcgtgt gcgtggagtg tctggtgaca
     1321 gcctccatcg atatgttccc taggcagctg aggaagtctg gcaggcgcga gctgctgatc
     1381 ctgaccatcg ccgtgatgtg ctacctgatc ggcctgtttc tggtgacaga gggcggcatg
     1441 tatatcttcc agctgtttga ttactatgcc tctagcggca tctgtctgct gttcctgagc
     1501 ctgtttgagg tggtgtgcat ctcctgggtg tacggcgccg accgcttcta tgataacatc
     1561 gaggacatga tcggctaccg gccttggcca ctggtgaaga tcagctggct gtttctgacc
     1621 cctggcctgt gcctggccac atttctgttc tctctgagca agtacacccc actgaagtat
     1681 aacaacgtgt acgtgtaccc accctggggc tactccatcg gctggttcct ggccctgtcc
     1741 tctatggtgt gcgtgcccct gtttgtggtc atcaccctgc tgaagacaag aggcccattc
     1801 cggaagagac tgaggcagct gatcacaccc gacagctccc tgccccagcc taagcagcac
     1861 ccttgcctgg atggctctgc cggcagaaat ttcggcccaa gcccaaccag ggagggactg
     1921 atcgcaggag agaaggagac acacctgtaa gacccagctt tcttgtacaa agttggcatt
     1981 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2041 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2101 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2161 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2221 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2281 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2341 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2401 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2461 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2521 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2581 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2641 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2701 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2761 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2821 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2881 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2941 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3001 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3061 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3121 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4021 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4081 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4141 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4201 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4261 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4321 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4381 cttaagctcg ggccc
//
