LOCUS       sequence-345428-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9A6_STOP
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2117..2216)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2233..2252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2234..2252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2257..2273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2375..2394)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2386..3195
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2453..2472)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3342..3930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3831..3850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4084..4101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4260..4287)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4379..4465)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4514..4536
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4528..4545
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4529..4545
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc tcggagaggc
      121 tggaggcgcg cccctctgcg gagaggagtg ggaagctccc ctagggcaag gcgcctgatg
      181 agaccactgt ggctgctgct ggcagtgggc gtgttcgatt gggcaggagc atctgacgga
      241 ggaggaggag aggcccgggc catggatgag gagatcgtga gcgagaagca ggccgaggag
      301 tcccacagac aggactctgc caatctgctg atcttcatcc tgctgctgac actgaccatc
      361 ctgaccatct ggctgtttaa gcaccggaga gccaggttcc tgcacgagac aggcctggcc
      421 atgatctacg gactgctggt gggactggtg ctgcgctatg gcatccacgt gccatccgat
      481 gtgaacaatg tgaccctgtc ttgcgaggtg cagtctagcc ccaccacact gctggtgaca
      541 ttcgaccctg aggtgttctt taatatcctg ctgcccccta tcatctttta cgccggctat
      601 tccctgaaga ggcgccactt ctttcggaac ctgggctcta tcctggccta cgcctttctg
      661 ggcaccgcca tcagctgctt cgtgatcggc tccatcatgt atggctgcgt gaccctgatg
      721 aaggtgaccg gccagctggc cggcgatttc tactttaccg actgtctgct gttcggcgcc
      781 atcgtgtccg ccacagatcc agtgaccgtg ctggccatct tccacgagct gcaggtggac
      841 gtggagctgt atgccctgct gtttggcgag agcgtgctga atgacgccgt ggccatcgtg
      901 ctgtcctcta gcatcgtggc ataccagcca gcaggcgata actctcacac atttgacgtg
      961 accgccatgt tcaagagcat cggcatcttc ctgggcatct tttctggcag cttcgccatg
     1021 ggagcagcaa caggagtggt gaccgccctg gtgacaaagt ttaccaagct gagggagttc
     1081 cagctgctgg agacaggcct gttctttctg atgtcctggt ctacctttct gctggcagag
     1141 gcatggggct tcacaggagt ggtggccgtg ctgttttgcg gcatcacaca ggcccactac
     1201 acctataaca atctgagcac agagtcccag caccgcacca agcagctgtt tgagctgctg
     1261 aacttcctgg ccgagaattt catcttttcc tatatgggcc tgacactgtt cacctttcag
     1321 aaccacgtgt ttaatcctac cttcgtggtg ggcgcctttg tggccatctt cctgggcagg
     1381 gccgccaaca tctacccact gagcctgctg ctgaatctgg gccggagatc taagatcggc
     1441 agcaattttc agcacatgat gatgttcgca ggactgaggg gagcaatggc cttcgccctg
     1501 gccatcaggg atacagccac ctacgcccgc cagatgatgt tctccaccac actgctgatc
     1561 gtgttcttta ccgtgtgggt gtttggagga ggaaccacag caatgctgag ctgcctgcac
     1621 atccgggtgg gcgtggattc cgaccaggag cacctgggag tgccagagaa cgagaggagg
     1681 accacaaagg cagagtccgc ctggctgttt agaatgtggt acaacttcga ccacaattat
     1741 ctgaagccac tgctgaccca ctctggacca ccactgacca caaccctgcc agcatgctgt
     1801 ggacctatcg caagatgtct gaccagccct caggcctatg agaaccagga gcagctgaag
     1861 gacgatgact ccgatctgat cctgaatgat ggcgacatct ctctgaccta cggcgacagc
     1921 acagtgaaca ccgagccagc cacatcctct gccccacgga gattcatggg caacagctcc
     1981 gaggatgcac tggacaggga gctggccttt ggcgatcacg agctggtcat caggggaacc
     2041 aggctggtgc tgccaatgga tgacagcgag cctccactga acctgctgga caatacaaga
     2101 cacggccccg cctaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     2161 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     2221 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2281 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2341 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2401 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2461 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2521 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2581 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2641 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2701 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2761 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2821 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2881 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2941 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     3001 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3061 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3121 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3181 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3241 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4081 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4141 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4201 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4261 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4321 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4381 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4441 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4501 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
