LOCUS       sequence-345429-        4947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9A4_STOP
FEATURES             Location/Qualifiers
     source          1..4947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2504..2603)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2620..2639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2621..2639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2644..2660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2644..2660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2762..2781)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2773..3582
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2840..2859)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3729..4317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4218..4237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4471..4488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4647..4674)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4766..4852)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4901..4923
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4915..4932
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4916..4932
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cctgcagatg
      121 ttcgtgacct actctccctg gaactgcctg ctgctgctgg tggccctgga gtgtagcgag
      181 gccagctccg atctgaacga gtctgccaat agcacagccc agtatgccag caatgcatgg
      241 ttcgcagcag catctagcga gccagaggag ggcatctccg tgtttgagct ggattacgac
      301 tatgtgcaga tcccctacga ggtgaccctg tggatcctgc tggcctctct ggccaagatc
      361 ggcttccacc tgtatcacag gctgcctggc ctgatgccag agagctgcct gctgatcctg
      421 gtgggcgccc tggtgggagg catcatcttt ggcaccgatc acaagtcccc ccctgtgatg
      481 gactcctcta tctacttcct gtatctgctg ccaccaatcg tgctggaggg aggatacttt
      541 atgcccacac gccctttctt tgagaacatc ggctccatcc tgtggtgggc cgtgctgggc
      601 gccctgatca atgcactggg catcggcctg tctctgtatc tgatctgtca ggtgaaggcc
      661 ttcggcctgg gcgatgtgaa cctgctgcag aatctgctgt ttggctccct gatctctgcc
      721 gtggaccctg tggccgtgct ggccgtgttc gaggaggcac gggtgaacga gcagctgtac
      781 atgatgatct ttggcgaggc cctgctgaac gacggcatca ccgtggtgct gtataatatg
      841 ctgatcgcct tcaccaagat gcacaagttt gaggatatcg agacagtgga catcctggca
      901 ggatgcgcaa ggttcatcgt ggtgggactg ggaggcgtgc tgtttggcat cgtgttcggc
      961 tttatctccg ccttcatcac ccgctttaca cagaacatct ctgccatcga gcccctgatc
     1021 gtgttcatgt ttagctacct gtcctatctg gcagcagaga ccctgtacct gtccggaatc
     1081 ctggcaatca ccgcatgtgc agtgacaatg aagaagtatg tggaggagaa cgtgagccag
     1141 acaagctaca ccacaatcaa gtatttcatg aagatgctga gctccgtgag cgagaccctg
     1201 atcttcatct ttatgggcgt gtccacagtg ggcaagaacc acgagtggaa ttgggccttc
     1261 atctgcttta ccctggcctt ttgtcagatc tggcgggcca tcagcgtgtt cgccctgttt
     1321 tacatcagca accagttcag aacattccct ttttctatca aggatcagtg catcatcttt
     1381 tatagcggcg tgaggggagc aggatctttc agcctggcct ttctgctgcc tctgagcctg
     1441 ttcccacgca agaagatgtt tgtgaccgcc acactggtgg tcatctactt caccgtgttc
     1501 atccagggca tcacagtggg cccactggtg cggtatctgg acgtgaagaa gaccaacaag
     1561 aaggagtcca tcaatgagga gctgcacatc agactgatgg atcacctgaa ggcaggaatc
     1621 gaggacgtgt gcggacactg gagccactac caggtgaggg ataagttcaa gaagtttgac
     1681 cacaggtatc tgcgcaagat cctgatccgc aagaatctgc ccaagtctag catcgtgtcc
     1741 ctgtacaaga agctggagat gaagcaggcc atcgagatgg tggagaccgg catcctgtcc
     1801 tctacagcct tcagcatccc ccaccaggcc cagaggatcc agggcatcaa gcgcctgtct
     1861 cctgaggatg tggagagcat cagagacatc ctgacctcta acatgtacca ggtgcggcag
     1921 agaacactga gctacaacaa gtataatctg aagcctcaga cctccgagaa gcaggccaag
     1981 gagatcctga tccggagaca gaatacactg agggagtcca tgcgcaaggg acactctctg
     2041 ccatggggca agcctgcagg aaccaagaac atccggtacc tgtcctaccc atatggcaat
     2101 ccccagtctg ccggaaggga taccagagca gcaggcttca gcgacgatga cagctccgac
     2161 ccaggctccc cctctatcac attttccgcc tgctctagaa tcggctccct gcagaagcag
     2221 gaggcccagg agatcatccc aatgaagtct ctgcacaggg gccgcaaggc cttcagcttt
     2281 ggctaccagc ggaacacctc ccaggaggag tatctgggag gcgtgaggag ggtggccctg
     2341 agacctaagc cactgttcca cgcagtggat gaggagggag agagcggagg agagtccgag
     2401 ggcaaggcaa gcctggtgga ggtgcggtcc agatggacag cagatcacgg acacggaagg
     2461 gaccaccaca gaagccactc ccccctgctg cagaagaagt aagacccagc tttcttgtac
     2521 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     2581 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2641 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2701 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2761 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     2821 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     2881 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     2941 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3001 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3061 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3121 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3181 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3241 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3301 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3361 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3421 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3481 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3541 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3601 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3661 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3841 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4381 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4441 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4501 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4561 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     4621 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     4681 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     4741 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     4801 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     4861 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     4921 acgacggcca gtcttaagct cgggccc
//
