LOCUS       sequence-345431-        5633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp12
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2944..3043)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3060..3079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3061..3079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3084..3100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3084..3100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3531..4322
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4415..5003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4904..4923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5157..5174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5333..5360)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5452..5538)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5587..5609
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5601..5618
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5602..5618
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct caaaagatac taatttttta aaccgggttc ggggtacgag tgtagatgcc
      181 cgtctcgtac cctgcgccag tggtttatct actgatgtac aattaagggc atttgatatt
      241 tacaatgcta gtgttgctgg cattggttta catttaaaag ttaattgttg ccgttttcag
      301 cgtgttgatg agaacggtga taaattagat cagttctttg ttgttaagag gacagatctg
      361 actatatata atagagagat gaaatgctat gagcgtgtaa aagattgtaa gtttgtggct
      421 gaacacgatt tctttacatt tgatgtagaa ggtagtcgtg tgccacacat tgtacgcaag
      481 gatttaacaa agtatactat gttggatctt tgctatgcat tgcgacattt tgatcgcaat
      541 gattgcatgc tgctttgtga cattctctct atatatgctg gttgtgaaca atcctacttt
      601 actaagaagg attggtatga ttttgttgaa aatcctgata ttattaatgt gtataaaaag
      661 ctaggaccta tttttaatag agccctagtt agcgctactg agtttgcgga caaattggtg
      721 gaggtaggct tagtaggcgt tttaacactt gataatcaag atttaaatgg taaatggtat
      781 gattttggtg actatgttat tgcagcccca ggatgtggtg ttgctatagc agattcttat
      841 tattcttata tcatgcctat gctgaccatg tgtcatgcat tggattgcga attgtatgtg
      901 aataatgctt atagactatt tgatcttgta cagtatgatt ttactgatta caagcttgaa
      961 ttgtttaata agtattttaa gcactggagt atgccatatc atcctaacac tgttgattgt
     1021 caggatgatc ggtgtattat acattgtgct aattttaaca tactttttag tatggtttta
     1081 cctaatacat gttttgggcc tcttgttagg caaatttttg tggatggtgt gccttttgtt
     1141 gtttcaattg gctaccatta taaagaactt ggtattgtga tgaatatgga tgtggataca
     1201 catcgttatc gcttgtcttt aaaagacttg cttttatatg ctgctgatcc agctttgcat
     1261 gtagcttctg ctagtgcatt gtatgattta cgcacttgct gttttagtgt tgccgctata
     1321 acaagcggtg taaaatttca aacagttaaa cctggtaatt ttaatcagga tttttatgat
     1381 tttgttttaa gtaaaggcct gcttaaagag ggtagctcag ttgatctgaa gcactttttc
     1441 tttacacagg atggtaatgc tgctattact gattataatt attataagta taatttgccc
     1501 accatggtgg acattaagca gttgttgttt gttttggaag ttgtttataa gtattttgag
     1561 atttatgatg gtgggtgtat accggcatca caagtcattg ttaataatta tgataagagt
     1621 gctggctatc catttaacaa atttggaaaa gccaggctct attatgaagc attatcattt
     1681 gaggaacagg atgaaattta cgcttatact aagcgtaatg tcctgccaac acttactcaa
     1741 atgaatttga aatatgctat tagtgctaag aatagagccc gcactgttgc tggtgtttcc
     1801 atacttagta ctatgactgg cagaatgttt catcaaaaat gtttgaaaag tatagcagct
     1861 acacgtggtg ttcctgtagt tataggcacc actaaatttt atggtggctg ggatgatatg
     1921 ttacgccgcc ttattaaaga tgttgacaat cctgtactta tgggttggga ttatcctaag
     1981 tgtgatcgtg ctatgccaaa cctactacgt attgttagta gtttggtatt agcccgaaaa
     2041 catgagacat gttgttcgca aagcgatagg ttttatcgac ttgcgaatga atgcgcacaa
     2101 gttttgagtg aaattgttat gtgtggtggc tgttattatg ttaagcctgg tggcactagt
     2161 agtggtgatg caactactgc ttttgctaat tcagtcttta acatatgtca agctgtttca
     2221 gccaatgtat gtgccttaat gtcatgcaat ggcaataaga ttgaagatct tagtatacgt
     2281 gctcttcaga agcgcttata ctcacatgtg tatagaagtg ataaggttga ttcaaccttt
     2341 gtcacagaat attatgaatt tttaaataag cattttagta tgatgatttt gagtgatgat
     2401 ggggttgtgt gttataattc tgattatgcg tccaaagggt atattgctaa tataagtgcc
     2461 tttcaacagg tattatatta tcaaaataac gtttttatgt cagaatccaa atgttgggtt
     2521 gaacatgaca taaataatgg acctcatgaa ttctgttcac aacacacaat gcttgtaaag
     2581 atggatggtg acgatgtcta ccttccatat cctaatccta gtcgtatatt aggagctgga
     2641 tgttttgtag atgatttgtt aaagactgat agtgttcttt taatagaacg atttgtaagt
     2701 cttgcaatag atgcttatcc acttgtgtat catgaaaatg aagaatacca aaaggttttt
     2761 cgtgtttatt tggcgtatat aaagaagttg tacaatgacc tgggtaatca gatcttggat
     2821 agctacagtg ttattttaag tacttgtgat ggacaaaagt tcactgatga gtccttttac
     2881 aagaacatgt atttaagaag tgcagttatg cagcttaatt aagacgcgat cgcggcctca
     2941 tgggcccagc tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt
     3001 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     3061 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3121 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     3181 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     3241 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3301 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3361 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3421 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3481 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3541 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3601 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3661 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3721 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3781 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3841 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3901 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3961 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     4021 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     4081 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     4141 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     4201 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     4261 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4321 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4561 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4801 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5041 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5101 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5161 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5221 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5281 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     5341 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5401 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5461 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5521 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     5581 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccc
//
