LOCUS       sequence-345456-        6503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0880:Dendra
FEATURES             Location/Qualifiers
     source          1..6503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2283..2306
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2563..3363
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3488..4076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3977..3996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6385..6404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6385..6403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgctgggccc caagggtcgc aacgtcgtcc tggagaagaa gtggggcgcc cccacgatca
       61 ccaacgatgg tgtgtccatc gccaaggaga tcgagctcga ggacccctac gagaagatcg
      121 gtgctgagct cgtcaaagag gtcgccaaga agaccgacga tgtcgctggc gacggcacca
      181 ccaccgccac cgtcctggct caggccctgg ttcgcgaagg cctgcgcaac gtcgctgccg
      241 gcgccaaccc gctcggcctg aagcgcggca tcgagaaggc cgtcgagaag gtcaccgaga
      301 ccctgctgaa gtccgccaag gaggtggaga ccaaggagca gatcgctgcc accgccggta
      361 tctccgccgg tgaccagtcc atcggcgacc tgatcgccga ggccatggac aaggtcggca
      421 acgagggtgt catcaccgtc gaggagtcca acaccttcgg cctgcagctc gagctcaccg
      481 agggtatgcg cttcgacaag ggctacatct cgggttactt cgtgaccgac gccgagcgtc
      541 aggaagcggt cctcgaggat ccctacatcc tgctggtcag ctcgaaggtc tcgaccgtca
      601 aggacctgct gccgctgctg gagaaggtca tccagtccgg caagccgctg ctgatcatcg
      661 ccgaggacgt cgagggcgaa gccctgtcga ccctggtggt caacaagatc cgcggcacct
      721 tcaagtcggt cgccgtcaag gcccccggct tcggtgaccg ccgcaaggcg atgctgcagg
      781 acatggccat cctcaccggt ggtcaggtca tcagcgaaga ggtcggcctg tccctggaga
      841 ccgctgacgt ctcgctgctg ggcaaggccc gcaaggtcgt cgtgaccaag gacgagacca
      901 cgatcgtcga gggtgccggc gacgccgagg ccatccaggg ccgcgtcgcg cagatccgcg
      961 ccgagatcga gaacagcgac tccgactacg accgcgagaa gctgcaggag cgcctggcca
     1021 agctggccgg cggtgttgcg gtcatcaagg ccggtgctgc caccgaggtg gagctcaagg
     1081 agcgcaagca ccgcatcgag gacgccgtgc gcaacgccaa ggccgccgtc gaggagggca
     1141 tcgtcgccgg tggcggcgtg gccctgctgc agtcggctcc gtcgctggag gagctgtcgc
     1201 tcaccggtga cgaggcgacc ggcgccaaca tcgtgcgcgt cgcgctgtcg gccccgctga
     1261 agcagatcgc cctcaacggt ggtctggagc cgggcgtcgt cgccgagaag gtgtcgaacc
     1321 tgcccgcggg tcacggcctg aacgccgcga ccggtgagta cgaggacctg ctggccgccg
     1381 gcgttgccga cccggtcaag gtcacccgct cggcgctgca gaacgctgcg tcgatcgcgg
     1441 ctctgttcct caccaccgag gctgttgtcg ccgacaagcc ggagaaggcc gccgcacctg
     1501 cgggcgaccc gaccggtggc atgggtggca tggacttcaa gcttggtgcg gccgcgggca
     1561 acacgcctgg catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca
     1621 acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga
     1681 cccagaccgc caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc
     1741 tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg
     1801 actacttcaa gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag
     1861 acaagggtat ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga
     1921 acgtccgttt caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc
     1981 tcaagtggga gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca
     2041 tcaacatggc gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt
     2101 acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa
     2161 tcctggggaa cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct
     2221 atagcccgct cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc
     2281 aggactacaa ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg
     2341 tcttgagggg ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga
     2401 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
     2461 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc
     2521 gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag
     2581 tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt
     2641 ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc
     2701 gaagatgggc cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg
     2761 ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc
     2821 gttacaccgg accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc
     2881 agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat
     2941 ccattgcccc tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat
     3001 gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag
     3061 ttgatggcaa aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag
     3121 ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac
     3181 aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg
     3241 ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg
     3301 atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt
     3361 ggctgagctc atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct
     3421 ggggcaacac gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaggggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct
     4261 ctagagtcga ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc
     4321 tcgctgagag ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc
     4381 gctgcaactc ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc
     4441 tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc
     4501 gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac
     4561 aagagttgca gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc
     4621 ggagcgggcg acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac
     4681 cacatatggg ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa
     4741 agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg
     4801 gtagccttgt tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa
     4861 gactcagcgc agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta
     4921 ctacgcgctg cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa
     4981 gcggctcatc gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc
     5041 cagcgccatc acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga
     5101 cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga
     5161 agtggcggtc acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag
     5221 gaagcacccg actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc
     5281 ggtcgaggac aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga
     5341 gcgcgacgta gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga
     5401 gcactaccgg atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat
     5461 cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg
     5521 tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa
     5581 gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg
     5641 tacgaagatg aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct
     5701 gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga
     5761 actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac
     5821 gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca
     5881 gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac
     5941 ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct
     6001 tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag
     6061 acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg
     6121 gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc
     6181 acggccgtgg gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg
     6241 cgcagtgagc tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg
     6301 tgagaagggt ctctgacgag cgggagaact ccctatcagt gatagagttt gtcctcccta
     6361 tcagtgatag ataggcttaa ggagtaatac gactcactat agggagaagg aggccattat
     6421 ggctaagaca attgcgtatg acgaagaggc ccgtcgcggc ctcgagcggg gcctcaacag
     6481 cctcgccgac gcggtaaagg tga
//
