LOCUS       sequence-345459-        5576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0919:Dendra
FEATURES             Location/Qualifiers
     source          1..5576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     324..343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        324..342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1798..1821
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2078..2878
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3003..3591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3492..3511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacgg
       61 tgaccacaac gcgcccgctt tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca
      121 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
      181 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
      241 gagaagggtc tctgacgagc gggagaactc cctatcagtg atagagtttg tcctccctat
      301 cagtgataga taggcttaag gagtaatacg actcactata gggagaagga ggccattatg
      361 gctgacaaga cccaacccac cgtggaagaa ctcaaggctc cgctgctcgc cgccgtcggc
      421 gccgccgatc tggcgctggc cacggtcaac gagatcatcg cgaccctgct cgagcgtgcc
      481 ggtgaggccc gcagcgacgc cgaggcccgc gtcgaggaga gccgcgcgcg cctgaccaag
      541 ctgcaggagg agctgcccag ccagttcggt gagctgcgcg agaagctgaa ctccgacgaa
      601 ctgcgcaaga agctgaactc cgaagagctg cgcaaggccg ccgagagcta cgccgaccag
      661 gccaccgcga cctacaacaa gctcgtcgag cgtggcgagg cggcgctgga gcgcctgcgc
      721 aaccagcctg cgctcgaaga ggccgcgacc cgcgtcgaga cctacaccga ccaggccgtc
      781 gagctgaccc aggaggccct gggcaccgtg gcgtcgcaga cccgcgccgt cggcgagcgt
      841 gccgccaagc tggtgggcgt cgaactgccg aagcgtgccg aggccgccgc cgagaccgcc
      901 tcggaggccg ccgccgagac cgccgagccg gtcaagaagg ctgccgctcc ggccgccaag
      961 aaggccgccc ccgccaagaa ggccgctccg gccaagaagg ccaccccggc caagagcgcg
     1021 gccaaggccc cggccaagaa ggtcacccag aagaagcttg gtgcggccgc gggcaacacg
     1081 cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg
     1141 aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga agggacccag
     1201 accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga tatcctcact
     1261 accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat ccctgactac
     1321 ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt cgaagacaag
     1381 ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt ccagaacgtc
     1441 cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa gaccctcaag
     1501 tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg caacatcaac
     1561 atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac cacgtacaaa
     1621 gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg
     1681 gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc acgctatagc
     1741 ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta tttccaggac
     1801 tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa acgggtcttg
     1861 aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta actgatgccg
     1921 tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg
     1981 aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     2041 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
     2101 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
     2161 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
     2221 tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag ggacgctcgt
     2281 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
     2341 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
     2401 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     2461 gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     2521 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     2581 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     2641 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     2701 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     2761 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     2821 cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg
     2881 agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc
     2941 aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat caaaggatct
     3001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3121 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgag
     3421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3661 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3721 cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga
     3781 gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag ccagctcgct
     3841 gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc cgaccgctgc
     3901 aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc caatctggtg
     3961 tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag
     4021 tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag
     4081 ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga ccagcggagc
     4141 gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg ccgaccacat
     4201 atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa
     4261 ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc
     4321 cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc
     4381 agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg
     4441 cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc
     4501 tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg
     4561 ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca
     4621 ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg
     4681 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
     4741 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
     4801 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
     4861 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact
     4921 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
     4981 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
     5041 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
     5101 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
     5161 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga
     5221 agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc
     5281 gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca
     5341 aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg
     5401 cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct
     5461 gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca
     5521 gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggaga
//
