LOCUS       sequence-345462-        5888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0777:Dendra
FEATURES             Location/Qualifiers
     source          1..5888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2498..2521
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2778..3578
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3703..4291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4192..4211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtgaccacg acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg
       61 tggctacgcc aagatcggtc ggccggaact ccgcatccac gacctccgcg ctgtcggcgc
      121 tacgttcgcc gctcaggcag gtgcgacgac caaggagctg atggcccgtc tcggtcacac
      181 gactcctagg atggcgatga agtaccagat ggcgtctgag gcccgcgacg aggctatcgc
      241 tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca aaaagccccc ctcccaagga
      301 cactgagtcc taaagagggg ggtttcttgt cagtacgcga agaaccacgc ctggccgcga
      361 gcgccagcac cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca
      421 ttgccgttcc cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg
      481 tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg
      541 atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc
      601 tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc
      661 tatcagtgat agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac
      721 tcactatagg gagaaggagg ccattatggc tgaattgaag ctaggttaca aggcatctgc
      781 ggagcagttt gctcctcgcg agttggtcga gttggcggtt ttggccgagt cggcaggtat
      841 ggacagtgcg actgtcagtg atcatttcca gccgtggcgt cacgaaggtg ggcatgcgcc
      901 gttctcgctg gcgtggatga ccgcggtggg cgagcgcacc aagaacctgg tgctggggac
      961 gtcggtgctg acgccgacgt tccggtacaa cccggcggtg atcgcccagg cgttcgcgac
     1021 gatgggatgt ctgtatccgg ggcggatctt cctcggtgtg ggcaccggtg aggcgctcaa
     1081 cgagatcgcg accgggtatg ccggcgagtg gccggagttc aaggagcggt tcgcgcggct
     1141 acgcgaatcg gtgcggttga tgcgtgagct gtggctgggg gaccgggtcg atttcgacgg
     1201 cgagtactac cgcaccaagg gcgcgtcgat ctatgacgtg cccgagggtg gcatcccggt
     1261 gtacatcgcc gcgggtggtc cggtggtggc caagtacgcc ggccgtgccg gtgacgggtt
     1321 catctgcacc tcgggcaagg gtgaggagct ctatgccgag aagctgatcc cggcggtcaa
     1381 ggagggtgct gcggccgccg atcgcgacgc cgacgcgatc gaccggatga tcgagatcaa
     1441 gatctcctat gacaccgatc ccgagttggc gctggagaac acccggttct gggcgccgct
     1501 gtcgttgacc gccgagcaga agcattcgat cgacgatccg atcgagatgg agaaggctgc
     1561 cgacgcgttg cccatcgagc aggtcgccaa gcgctggatc gtcgcgtcgg accccgacga
     1621 ggcggtcgag aaggtcggcc agtacgtcaa gtgggggctg aaccacctgg tgttccacgc
     1681 cccgggccat gaccagcgtc gattcctcga gctgttcaaa cgcgatctgg aaccgcggtt
     1741 gcgcaagctg gccaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1801 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1861 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1921 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1981 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     2041 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     2101 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     2161 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     2221 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     2281 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     2341 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     2401 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     2461 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     2521 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     2581 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     2641 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2701 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2761 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2821 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2881 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2941 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     3001 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     3061 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     3121 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     3181 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     3241 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     3301 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     3361 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     3421 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3481 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3541 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     3601 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3661 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3841 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4081 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     4141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4441 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     4501 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     4561 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     4621 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4681 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4741 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4801 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4861 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4921 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4981 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     5041 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     5101 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     5161 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     5221 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     5281 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     5341 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     5401 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     5461 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     5521 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     5581 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     5641 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5701 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5761 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5821 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5881 gcattcct
//
